Abstract: The present invention provides an electrochemical biosensor comprising a carbon nanotube for measuring biosignals. The electrochemical biosensor for continuous glucose monitoring comprises an electrode to which a sensing film including an oxidoreductase, an electron transfer mediator, and a crosslinker is fixed, together with a carbon nanotube, wherein the oxidoreductase oxidizes a target substance and the electron thus generated in the oxidation process is transferred through the electron transfer mediator and the carbon nanotube, whereby the electrochemical biosensor can be used as a sensor having an excellent performance for continuous glucose monitoring.