Abstract: Variant chemoautotrophic bacteria of the genus Xanthobacter including a genetic modification that reduces the bacterial production of polyhydroxyalkanoic acids. Furthermore, the present disclosure relates to continuous culture processes for the production of protein or biomass using variant chemoautotrophic bacteria, said process including supply of gases and minerals to the cells. The present disclosure also relates to the products of these processes and use of these products in e.g. food or feed. Reference is made to the Identification of the Microorganism, having the Identification reference given by the DEPOSITOR of SoF1-2.0 and with the Accession number given by the INTERNATIONAL DEPOSITORY AUTHORITY of VTT E-213595. The date of the original deposit is Apr.

Abstract: A method of growing a microbial mass includes collecting biowaste from an organism, the biowaste comprising a first amount of water and a first amount of solid-phase; receiving the collected biowaste in a first reactor; treating the collected biowaste in the first reactor using a first set of operating parameters for a first period of time to dissolve at least a part of solid-phase inorganic nutrients into the first amount of water to form a growth media; washing the formed growth media, wherein said washing includes sterilization, separation and cleaning steps; providing the formed growth media to a second reactor including an inoculum of microbial mass; collecting carbon dioxide locally from an atmosphere; collecting a second amount of water present in the atmosphere and splitting the collected second amount of water into oxygen and hydrogen gas; providing the collected carbon dioxide and the split oxygen and hydrogen gas to the second reactor; growing a microbial mass in the second reactor under a second s

Abstract: A method of producing a cell growth medium. The method includes cultivating microbial cells by gas fermentation to obtain a biomass slurry, concentrating the biomass slurry by separating and removing a liquid phase from a solid phase, homogenizing the concentrated biomass slurry with high pressure homogenization, wherein the microbial cells are at least partially degrading, treating the homogenized biomass slurry by adding at least one protease, and heating the treated biomass slurry. Reference is made to the Identification of the Microorganism, with the identification reference given by the DEPOSITOR of SoF1, having the Accession number given by the INTERNATIONAL DEPOSITORY AUTHORITY of VTT E-193585, received on Jun. 11, 2019, (date of original deposit).

Abstract: A method of producing a meat analogue food ingredient. The method includes a downstream process including cultivating bacterial cells to obtain a biomass, separating a liquid phase and a solid phase of the biomass, concentrating the biomass by removing the liquid phase; and drying the biomass to obtain the first protein powder; mixing a first protein powder with a liquid and NaCl to obtain a powder mixture; extruding the powder mixture with high-moisture extrusion; cutting the extruded mixture; and cooling the extruded mixture.

Abstract: Disclosed is a method of adding a feed medium into a bioprocess. The method includes receiving a stream of CO2-rich gas; treating the stream of CO2-rich gas to remove impurities therefrom; preparing an aqueous mixture for absorbing carbon dioxide, the aqueous mixture having at least one inorganic nitrogen compound in a range of 0.1-50 wt % of the aqueous mixture, the at least one inorganic nitrogen compound is a nitrogen source for microorganisms; absorbing carbon dioxide from the stream of CO2-rich gas into the aqueous mixture, the aqueous mixture with absorbed carbon dioxide forming a feed medium; and adding the feed medium into a bioprocess.

Abstract: Disclosed is a method of producing a meat analogue food product that includes mixing a microbial biomass protein slurry with a preparation with transglutaminase enzyme to obtain a protein mixture; incubating the protein mixture for a first period time with mixing at a temperature ranging from 28° C. up to 40° C.; adding at least one of selected from an aqueous MgCl2 or an aqueous CaCl2 to the protein mixture; incubating the protein mixture for a second period of time; incubating the protein mixture for a third period of time in a water bath at a temperature ranging from 40° C. up to 60° C.; heating the protein mixture at a temperature ranging from 60° C. up to 85° C.; and setting the protein mixture in a closed mold.

Abstract: Disclosed is a method of producing an egg replacement food product that includes mixing a protein microbial biomass powder with water to obtain a wet mixture, hydrating the wet mixture at room temperature, mixing the wet mixture at high speed, hydrating the mixed wet mixture at 6 C degrees, mixing the hydrated wet mixture with an oil at high speed and emulsifying the mixed wet mixture, homogenizing the emulsified mixed wet mixture, heating the homogenized wet mixture up to from 85 C up to 90 C degrees, and adding sodium chloride (NaCl) and citric acid to the heated wet mixture and mixing to obtain pH from 3.5 up to 4.0.

Abstract: A method of producing a nutritional supplement from microbial cells. The method includes cultivating microbial cells to obtain a biomass; incubating the biomass with a heat treatment at temperature from 55° C. up to 80° C. for an incubation time from 10 minutes up to 60 minutes; concentrating the biomass by separating and removing a liquid phase from a solid phase to obtain a dry matter content from 2% up to 40% of total weight of the nutritional supplement. Disclosed also is a system of producing a nutritional supplement from microbial cells using the aforementioned method. The system includes a bioreactor; a heat-exchanger and a separator for carrying out the respective steps of the aforementioned method. Reference is made to the Identification of the Microorganism, with the identification reference given by the Depositor SoF1, having the Accession number given by the INTERNATIONAL DEPOSITORY AUTHORITY, VTT E-193585, received on Jun.

Abstract: A bacterial strain of the genus Xanthobacter and continuous culture processes for the production of protein or biomass using bacteria of the genus Xanthobacter, said process including supply of gases and minerals to the cells. The present disclosure also relates to the products of these processes and use of these products in e.g. food or feed. Reference is made to the Identification of the Microorganism, having the Identification reference given by the DEPOSITOR of SoF1 and with the Accession number given by the INTERNATIONAL DEPOSITORY AUTHORITY of VTT E-193585. The date of the original deposit is Jun.

Abstract: A power converter for a bioelectrochemical system includes first converters each including a direct current terminal for supplying electric current via electrodes of the bioelectrochemical system, and a second converter for supplying energy to the first converters from an external electric power grid. Each first converter includes an electric element for receiving energy from the second converter and a circuitry for converting voltage of the electric element into electrolysis voltage suitable for the bioelectrochemical system. The electric element can be a secondary winding of a transformer or a direct voltage energy storage. Each first converter is galvanically isolated from the other first converters at least when the first mentioned first converter supplies energy to the bioelectrochemical system. Thus, each first converter drives its own electrode pair without disturbing the other first converters.

Abstract: A bioreactor for growing micro-organisms in a reaction mixture including a reaction medium and micro-organisms. The bioreactor includes a first reaction chamber, a second reaction chamber and means for connecting the first reaction chamber to the second reaction chamber. The first reaction chamber has first volume for containing first number of micro-organisms, a first input for providing reaction mixture thereto, and a first output for removing excess gases therefrom. The second reaction chamber, arranged downstream from first reaction chamber, has a second volume for containing a second number of micro-organisms, a second input for providing gases thereto, and a second output for removing reaction mixture therefrom. The means for connecting is the sole input for allowing the reaction mixture to flow from the first reaction chamber to the second reaction chamber and for gases to flow from the second reaction chamber to the first reaction chamber.

Abstract: A bioreactor for growing micro-organisms, has a reaction chamber containing a reaction mixture with a reaction medium and micro-organisms. A draft tube is arranged inside the reaction chamber, which has a gas inlet, an inlet for the reaction mixture at its first end, and an outlet for the reaction mixture at its second end. The bioreactor includes means for generating flow of the reaction mixture within the reaction chamber and a first blade structure arranged inside the reaction chamber, surrounding the draft tube. The first blade structure has blades arranged at, at least one of an angle ?1 with respect to a direction defined by the height of the reaction chamber, or an angle ?2 with respect to a direction defined by the height of the reaction chamber. The bioreactor also includes an inlet for reaction medium and an outlet for withdrawing medium with grown micro-organisms.