Patents Assigned to Solazyme, Inc.
  • Publication number: 20170044090
    Abstract: This invention relates to a ring opening method. The method includes reacting an epoxidized fatty acid ester with an alcohol to open an epoxy ring of the ester. A salt of tetrafluoroboric acid is used as a catalyst in the reaction. In another embodiment, the invention relates to a method of producing a polyurethane. An epoxidized fatty acid ester is reacted with an alcohol to open an epoxy ring of the ester, thereby to produce a polyol. A salt of tetrafluoroboric acid is used as a catalyst in the reaction. The polyol is then reacted with an isocyanate to produce a polyurethane. In a further embodiment, the polyol is used to produce a product selected from polyurethanes, coatings, adhesives, sealants, elastomers and lubricants.
    Type: Application
    Filed: October 29, 2014
    Publication date: February 16, 2017
    Applicant: Solazyme, Inc.
    Inventors: Herman P. Benecke, Jeffrey T. Cafmeyer, Daniel B. Garbark
  • Patent number: 9434909
    Abstract: The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel.
    Type: Grant
    Filed: February 19, 2014
    Date of Patent: September 6, 2016
    Assignee: SOLAZYME, INC.
    Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
  • Publication number: 20160251685
    Abstract: The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.
    Type: Application
    Filed: March 5, 2016
    Publication date: September 1, 2016
    Applicant: Solazyme, Inc.
    Inventors: George N. RUDENKO, Jason CASOLARI, Scott FRANKLIN
  • Patent number: 9375703
    Abstract: Provided are biomass-based materials and valuable uses of microalgal biomass including: (i) acetylation of microalgal biomass to produce a material useful in the production of thermoplastics; (ii) use of triglyceride containing microalgal biomass for production of thermoplastics; (iii) combination of microalgal biomass and at least one type of plant polymer to produce a material useful in the production of thermoplastics; (iv) anionization of microalgal biomass to form a water absorbant material; (v) cationization of microalgal biomass, and optional flocculation, to form a water absorbant material; (vi) crosslinking of anionized microalgal biomass; (vii) carbonization of microalgal biomass; and (viii) use of microalgal biomass in the making of paper.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: June 28, 2016
    Assignee: Solazyme, Inc.
    Inventors: Ali Harlin, Anna-stiina Jääskeläinen, Jani Kiuru, Christiane Laine, Tiina Liitiä, Kalle Nättinen, Jaakko Pere, Sonia Sousa, John Piechocki, Adrienne McKee, Jeffrey J. Cernohous, Adam R. Pawloski
  • Publication number: 20160177164
    Abstract: Provided are drilling fluids having delay-released lubrication, the drilling fluids comprising a drilling mud and an oleaginous microbial cell, methods of using and making such drilling fluids, and drilling rigs comprising such drilling fluids. Also provided are lubricants comprising an oleaginous microbial cell. Uses for the lubricants include metal working and extreme pressure applications.
    Type: Application
    Filed: February 12, 2016
    Publication date: June 23, 2016
    Applicant: Solazyme, Inc.
    Inventors: Harrison F. DILLON, Frederyk NGANTUNG, Ana Teresita ECHANIZ
  • Patent number: 9353389
    Abstract: Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.
    Type: Grant
    Filed: May 22, 2014
    Date of Patent: May 31, 2016
    Assignee: SOLAZYME, INC.
    Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
  • Patent number: 9345730
    Abstract: The invention is directed to methods of using Chlorella protothecoides microalgal biomass in the treatment of individuals having impaired glucose metabolism. In some cases, the patient has impaired fasting glucose, impaired glucose tolerance, or diabetes. In some methods, algal biomass is used to reduce blood glucose and/or body fat in a subject, or to increase the relative abundance of beneficial gut microflora in a subject. In preferred embodiments, the biomass is derived from Chlorella protothecoides cultures grown heterotrophically in which the algal cells comprise at least 15% algal oil by dry weight.
    Type: Grant
    Filed: April 30, 2014
    Date of Patent: May 24, 2016
    Assignees: SOLAZYME, INC., UNIVERSITY OF MANITOBA
    Inventors: David Brinkmann, Anthony G. Day, Peter J. H. Jones, Scott Harding
  • Patent number: 9328351
    Abstract: Genetically engineered microbial, e.g., Prototheca, cells provide microbial oil useful as a food additive and a source of renewable fuels and industrial chemicals.
    Type: Grant
    Filed: November 28, 2012
    Date of Patent: May 3, 2016
    Assignee: SOLAZYME, INC.
    Inventors: Scott Franklin, Shane Brubaker, George N. Rudenko, Jeffrey L. Moseley, Xinhua Zhao, Tina T. Huynh, Riyaz Bhat, Matthew Shoa-Azar, Trung H. Nguyen, Karen Espina, Aravind Somanchi
  • Publication number: 20160083758
    Abstract: Novel plant acyl-ACP thioesterase genes of the FatB and FatA classes and proteins encoded by these genes are disclosed. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Expression of the novel and/or mutated FATB and FATA genes is demonstrated in oleaginous microalga host cells. Furthermore, a method for producing an oil elevated in one or more of C12:0, C14:0, C16:0, C18:0 and/or C18:1 fatty acids includes transforming a cell with novel and/or mutated FATB and/or FATA genes, e.g., having an N-terminal deletion. The cells produce triglycerides with altered and useful fatty acid profiles.
    Type: Application
    Filed: September 18, 2015
    Publication date: March 24, 2016
    Applicant: Solazyme, Inc.
    Inventors: Jason CASOLARI, George N. RUDENKO, Scott FRANKLIN, Xinhua ZHAO
  • Patent number: 9290749
    Abstract: The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: March 22, 2016
    Assignee: Solazyme, Inc.
    Inventors: George N. Rudenko, Jason Casolari, Scott Franklin
  • Patent number: 9279136
    Abstract: Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: March 8, 2016
    Assignee: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky
  • Patent number: 9255282
    Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
    Type: Grant
    Filed: May 13, 2014
    Date of Patent: February 9, 2016
    Assignee: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky
  • Patent number: 9249252
    Abstract: Provided are microalgal oils having a low polyunsaturated fatty acid profile and derivatives of the oils, including acids, esters, epoxides, hydroxylated acids and esters, urethanes, amides, and polymers thereof. Also provided are compositions comprising the oils and their derivatives and their uses in foodstuffs and in industrial and material applications. The compositions include flexible polyurethane foams prepared from the microalgal oils.
    Type: Grant
    Filed: April 25, 2014
    Date of Patent: February 2, 2016
    Assignee: Solazyme, Inc.
    Inventors: Frederyk Ngantung, Diza Braksmayer, Risha Bond, Jeffrey T. Cafmeyer
  • Patent number: 9249436
    Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
    Type: Grant
    Filed: February 2, 2012
    Date of Patent: February 2, 2016
    Assignee: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky, Xinhua Zhao, Riyaz Bhat
  • Patent number: 9249441
    Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
    Type: Grant
    Filed: September 1, 2014
    Date of Patent: February 2, 2016
    Assignee: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
  • Patent number: 9205040
    Abstract: The invention provides cosmetic compositions comprising microalgal biomass, whole microalgal cells, and/or microalgal oil in combination with one or more other cosmetic ingredients, and methods of making such compositions. In preferred embodiments, the microalgal components of the cosmetic compositions are derived from microalgal cultures grown heterotrophically and which comprise at least 10% oil by dry weight.
    Type: Grant
    Filed: August 30, 2013
    Date of Patent: December 8, 2015
    Assignee: Solazyme, Inc.
    Inventors: Geoffrey Brooks, Scott Franklin
  • Patent number: 9200307
    Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type.
    Type: Grant
    Filed: July 12, 2013
    Date of Patent: December 1, 2015
    Assignee: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
  • Publication number: 20150247081
    Abstract: Provided are drilling fluids having delay-released lubrication, the drilling fluids comprising a drilling mud and an oleaginous microbial cell, methods of using and making such drilling fluids, and drilling rigs comprising such drilling fluids. Also provided are lubricants comprising an oleaginous microbial cell. Uses for the lubricants include metal working and extreme pressure applications.
    Type: Application
    Filed: May 16, 2015
    Publication date: September 3, 2015
    Applicant: SOLAZYME, INC.
    Inventors: Harrison F. DILLON, Frederyk NGANTUNG, Ana Teresita ECHANIZ
  • Patent number: 9115332
    Abstract: The invention generally relates to the production of hydrocarbon compositions, such as a lipid, in microorganisms. In particular, the invention provides methods for extracting, recovering, isolating and obtaining a lipid from a microorganism and compositions comprising the lipid. The invention also discloses methods for producing hydrocarbon compositions for use as biodiesel, renewable diesel, jet fuel, and other materials.
    Type: Grant
    Filed: October 16, 2013
    Date of Patent: August 25, 2015
    Assignee: Solazyme, Inc.
    Inventors: Harrison F. Dillon, Dan Elefant, Anthony G. Day, Scott Franklin, Jon Wittenberg
  • Patent number: 9109239
    Abstract: Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
    Type: Grant
    Filed: October 11, 2012
    Date of Patent: August 18, 2015
    Assignee: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky