Abstract: The inventive apparatus is comprised of a means for producing cavitation in a complex reaction mixture to enhance the yield of the selected reaction product, a means for controlling the temperature of the complex reaction mixture, especially during cavitation, and a means for affecting magnetic separation of paramagnetic beads to which the selected reaction product is attached from the complex reaction mixture. In one embodiment, the compound(s) or molecule(s) is synthesized in situ, and isolated using the inventive apparatus. The apparatus finds use in the fields of solid phase organic synthesis, and for isolation and purification of a selected compound(s) or molecule (s), especially where automation is desired.

Abstract: This invention provides a method and apparatus for agitating magnetic particles, suspended in a solvent using an alternating magnetic field gradient. The apparatus has a housing chamber capable of holding one or more vials containing suspended paramagnetic particles, one or more magnetic field generating devices surrounding the housing chamber and a controller device for controlling the length of time and position of the magnetic field generated.

Abstract: An apparatus for facilitating the running of multiple simultaneous solid phase reactions for mounting on a temperature controllable water bath and a method of using this apparatus in the presence or absence of paramagnetic beads to perform chemical syntheses resulting in separation and recovery of a final desired reaction product is provided. The apparatus comprises a reaction plate, a reaction vessel for mounting in the reaction plate, a reaction plate holder, a screw-like rod, a mounting block attached to the reaction plate holder for operably receiving the screw-like rod, a motor, a sonication region in the water bath and a magnetic separation region in the water bath. Paramagnetic beads having reaction sites are introduced into the reaction vessels along with the appropriate solvent and one or more reactants.

Abstract: An immunoscintillating composition and method of radioimmunoassay is disclosed whereby polymer coated paramagnetic particles and binding agent such as an antibody are associated by chemical or physical means to provide solid scintillating immunoadsorbent particles which are paramagnetic. These particles are capable of selectively binding or retaining radioactive or labeled antigens or ligands and transmitting radioactive energy to a phosphor or photon emitting substance that is bound by chemical or physical means to the particles. Upon binding of the antigens or ligands to the surface of the particles the particles are separated from the unbound components in solution by application of a magnetic field. The luminescence emitted by the phosphor which is attached to the magnetically separated particles is measured by a scintillator counter and is directly proportional to the radioactive energy released by the labeled antigen bound to the antibody.

Abstract: This invention provides a process for the synthesis of a defined chemical entity, i.e., any desired chemical compound, in high yield using a solid support comprising a magnetic particle, in organic solvents. In general, a sequence of chemical synthetic steps are required to prepare the defined chemical entity bound to the particle. The particle comprises a resin bearing a high concentration of substituent pendant functional groups, to which the first reagent employed in the synthetic sequence binds; in subsequent reaction steps further reagents react with the growing chemical intermediate bound to the pendant functional groups until the defined chemical entity is obtained. The particle also comprises smaller paramagnetic or superpara-magnetic particles incorporated within the resin. The magnetic particle therefore responds to imposed magnetic field gradients, permitting ease of manipulation in steps involving removal of organic solvent.