Patents Assigned to Solvay Enzymes, Inc.
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Patent number: 5565348Abstract: An alkaline protease which is suitable for detergent formulations is produced by three strains of a species of Bacillus proteolyticus. The alkaline protease has the amino acid terminal sequence of Seq. ID NO.: 1 as follows: Ala-Gln-Ser-Val-Pro-Trp-Gly-Ile-Ser-Arg-Val-Gln-Ala-Pro-Ala-Ala-His-Asn-Ar g-Gly-. In addition, the alkaline protease has a molecular weight of 28 kdaltons, an isoelectric point from 10-11.5, an optimum pH for proteolytic activity at a pH in the range of 8.5 and 11.5, and retains at least 70% of its original activity after being held at a pH of 8.0 at a temperature of 43.degree. C. for a period of 11 days.Type: GrantFiled: February 17, 1995Date of Patent: October 15, 1996Assignee: Solvay Enzymes, Inc.Inventors: Ernest W. Boyer, Graham S. Byng
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Patent number: 5562900Abstract: Prophylactic and therapeutic agents for the prevention and treatment of viral-induced tumors and, in particular, compositions derived from an Aspergillus fermentation extract, and the use thereof, either directly or to prepare a medicament, for the prevention and treatment of viral-induced tumors in mammals. Such tumors include papilloma-induced tumors. The composition is topically administered.Type: GrantFiled: September 27, 1994Date of Patent: October 8, 1996Assignee: Solvay Enzymes, Inc.Inventors: Ernest W. Boyer, Robert L. Charles
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Patent number: 5543302Abstract: Proteases are susceptible to autolytic degradation. The method of the invention provides a method to identify susceptible autolysis sites which are cleaved to produce a few discrete fragments. Once identified, amino acid(s) at or in the vicinity of the cleavage site may be chemically modified or the DNA sequence capable of encoding the amino acid present at the site may be changed or deleted by site directed mutagenesis to produce an autolysis stable protease. The invention is particularly applicable to alkaline proteases which are produced in large quantities and used as detergent additives. Stable alkaline proteases could then be used in liquid detergent formulations.Type: GrantFiled: November 30, 1993Date of Patent: August 6, 1996Assignee: Solvay Enzymes, Inc.Inventors: George Boguslawski, Josephine Grosch, John W. Shultz, Sethuraman Subramanian
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Patent number: 5541097Abstract: A method for preparing an immobilized enzyme conjugate, whereby the enzyme is treated with a polyfunctional amine reactive material for forming a treated enzyme-containing adduct before being immobilized on a solid support which has been contacted with a solution of a polyamine compound. The method is especially preferred for use with glucoamylase, fungal .alpha.-amylase and .beta.-amylase. Immobilized enzyme conjugates formed by use of this method include treated enzyme-containing adducts. The immobilized enzyme conjugates disclosed herein are more stable and the enzymes immobilized therein are more tightly-held than those otherwise obtained and provided.Type: GrantFiled: February 9, 1995Date of Patent: July 30, 1996Assignee: Solvay Enzymes, Inc.Inventors: Oreste J. Lantero, Jack W. Brewer, Sharon M. Sarber
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Patent number: 5518917Abstract: Three strains of a species of Bacillus proteolyticus are provided. This bacterium produces an alkaline protease which is suitable for detergent formulations. The alkaline protease has the amino acid terminal sequence of Seq. ID NO.: 1 as follows: Ala-Gln-Ser-Val-Pro-Trp-Gly-Ile-Ser-Arg-Val-Gln-Ala-Pro-Ala-Ala-His-Asn-Ar g-Gly-. In addition, the alkaline protease has a molecular weight of 28 kdaltons, an isoelectric point from 10-11.5, an optimum pH for proteolytic activity at a pH in the range of 8.5 and 11.5, and retains at least 70% of its original activity after being held at a pH of 8.0 at a temperature of 43.degree. C. for a period of 11 days.Type: GrantFiled: January 12, 1994Date of Patent: May 21, 1996Assignee: Solvay Enzymes, Inc.Inventors: Ernest W. Boyer, Graham S. Byng
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Patent number: 5516689Abstract: An enzyme composition and a means of reducing the stickiness of honeydew contaminated cotton is disclosed. The composition includes, and the method uses, enzymes such as transglucosidases and pectinases which are capable of hydrolyzing sugars that make-up honeydew. Suitable transglucosidases include those obtained from Aspergillus niger. Particularly preferred is a transglucosidase obtained from Aspergillus niger ATCC 14916.Type: GrantFiled: April 30, 1993Date of Patent: May 14, 1996Assignee: Solvay Enzymes, Inc.Inventors: Oreste J. Lantero, Jr., Jayarama K. Shetty
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Patent number: 5500359Abstract: Prophylactic and therapeutic agents for the prevention and treatment of viral-induced tumors and, in particular, compositions derived from an Aspergillus fermentation extract, and the use thereof, either directly or to prepare a medicament, for the prevention and treatment of viral-induced tumors in mammals. Such tumors include papilloma-induced tumors. The composition is topically administered.Type: GrantFiled: September 27, 1993Date of Patent: March 19, 1996Assignee: Solvay Enzymes, Inc.Inventors: Ernest W. Boyer, Robert L. Charles
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Patent number: 5472861Abstract: A method for preparing an immobilized enzyme conjugate, whereby the enzyme is treated with a polyfunctional amine reactive material for forming a treated enzyme-containing adduct before being immobilized on a solid support which has been contacted with a solution of a polyamine compound. The method is especially preferred for use with glucoamylase, fungal .alpha.-amylase and .beta.-amylase. Immobilized enzyme conjugates formed by use of this method include treated enzyme-containing adducts. The immobilized enzyme conjugates disclosed herein are more stable and the enzymes immobilized therein are more tightly-held than those otherwise obtained and provided.Type: GrantFiled: January 9, 1995Date of Patent: December 5, 1995Assignee: Solvay Enzymes, Inc.Inventors: Oreste J. Lantero, Jack W. Brewer, Sharon M. Sarber
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Patent number: 5439817Abstract: A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20.degree. C. and a pH between pH 4.0 and pH 6.5 at constant agitation.Type: GrantFiled: January 21, 1993Date of Patent: August 8, 1995Assignee: Solvay Enzymes, Inc.Inventors: Jayarama K. Shetty, Chimanbhai P. Patel, Mary Ann Nicholson
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Patent number: 5405767Abstract: A novel compound for producing a purified enzyme precipitate and a method of preparing the purified enzyme precipitate, wherein the compound is an organic compound selected from the group consisting of carboxylic acids having at least 2 carboxyl groups, salts or esters of these carboxylic acids, amino acids, salts or esters of these amino acids.Type: GrantFiled: April 8, 1992Date of Patent: April 11, 1995Assignee: Solvay Enzymes, Inc.Inventors: Jayarama K. Shetty, Chimanbhai P. Patel
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Patent number: 5385837Abstract: Alkaline proteases derived from specific bacteria of the species Bacillus proteolyticus have enhanced stability and improved washing ability when blended in general detergents. Also disclosed are new bacteria producing these alkaline proteases. Additionally, there is also disclosed a process for the production of the alkaline proteases which comprises cultivating new bacteria and detergent compositions containing these alkaline proteases.Type: GrantFiled: January 13, 1994Date of Patent: January 31, 1995Assignee: Solvay Enzymes, Inc.Inventors: Ernest W. Boyer, Graham S. Byng
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Patent number: 5380661Abstract: The invention relates to an acid stable levan sucrase derived from Bacillus licheniformis NRRL B-19862. The levan sucrase is produced by culturing Bacillus licheniformis NRRL B-19862 in a suitable nutrient medium but in the absence of sucrose. The invention also discloses compositions containing the levan sucrase.Type: GrantFiled: October 19, 1993Date of Patent: January 10, 1995Assignee: Solvay Enzymes, Inc.Inventors: Robert L. Charles, Jayarama K. Shetty
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Patent number: 5334524Abstract: The present invention relates to an acid stable levan sucrase enzyme which is not induced by sucrose.The present invention relates to a process for the preparation of this enzyme and microorganisms producing the levan sucrase enzyme. The invention also provides compositions containing this enzyme.Type: GrantFiled: May 18, 1992Date of Patent: August 2, 1994Assignee: Solvay Enzymes, Inc.Inventors: Robert L. Charles, Jayarama K. Shetty
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Patent number: 5281526Abstract: The present invention relates to a method for the preparation of a purified amylase recovered from a fermentation broth, by the addition of a precipitation agent which is a halide salt, most preferably sodium or potassium chloride. According to a preferred embodiment, a further precipitation agent is added which is an organic compound, most preferably an alkyl ester of 4-hydroxybenzoic acid.The present invention also relates to the purified amylases so obtained and to the uses of these purified amylases.Type: GrantFiled: October 20, 1992Date of Patent: January 25, 1994Assignee: Solvay Enzymes, Inc.Inventors: Ivan C. Good, Chong Y. Kim, Jayarama K. Shetty, Kent M. Sproat
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Patent number: 5256557Abstract: A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20.degree. C. and a pH between pH 4.0 and pH 6.5 at constant agitation.Type: GrantFiled: December 27, 1991Date of Patent: October 26, 1993Assignee: Solvay Enzymes, Inc.Inventors: Jayarama K. Shetty, Chimanbhai P. Patel, Mary A. Nicholson
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Patent number: 5231017Abstract: The invention relates to a process for producing ethanol from raw materials, that contain fermentable sugars or constituents which can be converted into sugars, comprising the steps of:a-liquefaction of the raw materials in the presence of an alpha-amylase for obtaining liquefied mash,b-saccharification of the liquefied mash in the presence of a glucoamylase for obtaining hydrolyzed starch and sugars,c-fermentation of the hydrolyzed starch and sugars by yeast for obtaining ethanol, andd-recovering alcohol,a protease being introduced in the liquefied mash during the saccharification and/or in the hydrolyzed starch and sugars during the fermentation.The invention relates also to a composition containing a glucoamylase and an acid fungal protease.Type: GrantFiled: May 17, 1991Date of Patent: July 27, 1993Assignee: Solvay Enzymes, Inc.Inventors: Oreste J. Lantero, John J. Fish
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Patent number: 5118623Abstract: The methods of the invention can be used to identify sites of cleavage of an enzyme in the presence of hypochlorite. It has been found that such cleavage occurs at tryptophan. Chemical modifications or genetic manipulation to change or delete tryptophan can be done to produce a more stable enzyme which retains activity in the presence of hypochlorite. The invention is particularly applicable to alkaline proteases which are useful in detergent compositions.Type: GrantFiled: May 27, 1988Date of Patent: June 2, 1992Assignee: Solvay Enzymes, Inc.Inventors: George Boguslawski, John W. Shultz