Patents Assigned to Sophion Bioscience A/S
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Patent number: 11692994Abstract: A seal enhancer for improving the patch clamp seal in a patch clamp method or apparatus is provided. The internal solution comprises particular anions and the external solution comprises one or more metal ions at low concentration.Type: GrantFiled: October 29, 2021Date of Patent: July 4, 2023Assignee: SOPHION BIOSCIENCE A/SInventors: Lars Damgaard Løjkner, Anders Lindqvist, Daniel Rafael Sauter, Kadla Røskva Rosholm
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Publication number: 20230023752Abstract: A contractile tissue-based analysis device is provided, in which a strip of contractile tissue is supported by support structure. The support structure comprises a substantially planar base element, and first and second support pillars extending from said base element. An optical detection device is arranged on the side of the base element opposite to said support pillars, and is arranged to capture image data from at least one of the head portions of the support pillars. The motion of the support pillars induced by the strip of contractile tissue can thus be captured from below, i.e. through the planar base element.Type: ApplicationFiled: December 21, 2020Publication date: January 26, 2023Applicants: Sophion Bioscience A/S, Danmarks Tekniske UniversitetInventors: Rie Kjær Christensen, Sandra Wilson, Peder Skafte-Pedersen, Marie Louise Lamb Busk, Niels Bent Larsen, Christoffer Von Halling Laier
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Patent number: 11215606Abstract: A seal enhancer for improving the patch clamp seal in a patch clamp method or apparatus is provided. The internal solution comprises particular anions and the external solution comprises one or more metal ions at low concentration.Type: GrantFiled: December 4, 2017Date of Patent: January 4, 2022Assignee: SOPHION BIOSCIENCE A/SInventors: Lars Damgaard Løjkner, Anders Lindqvist, Daniel Rafael Sauter, Kadla Røskva Rosholm
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Patent number: 10519339Abstract: A single-step method for applying a coating to a polymeric substrate comprising: exposing a photoreactive compound and a polymeric compound in an aqueous solvent to ultraviolet radiation in the presence of the polymeric substrate to obtain a covalently bound coating thereon comprising a reaction product of the photoreactive compound and the polymeric compound. More particularly, a method of applying a coating to a polymeric substrate in order to reduce the adsorption of analytes in solution to the substrate by imparting properties to the substrate similar to the properties of the coating applied.Type: GrantFiled: September 27, 2013Date of Patent: December 31, 2019Assignee: Sophion Bioscience A/SInventors: Esben Kjaer Unmack Larsen, Niels Bent Larsen
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Patent number: 10195608Abstract: The invention describes a foil of polymeric material comprising a plurality of patch portions and a grid portion arranged between said patches. Each patch portion is connected to the grid portion by means of one or more connecting elements. Each patch portion is separated from the grid portion in the X-Y plane by means of a release portion, said release portion having a tensile strength in the X-Y plane which is lower than the tensile strength of both said patch portions and said grid portion, such that—upon exertion of a tensile force between said patch portions and said grid portion—said foil ruptures preferentially at said release portion. The invention relates to a method for the production of cell-capture chips using said foil, and a cell capture chip produced by said method.Type: GrantFiled: February 25, 2015Date of Patent: February 5, 2019Assignee: Sophion Bioscience A/SInventors: Sandra Wilson, Anders Brask
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Patent number: 10006902Abstract: The present invention relates to a structure comprising a biological membrane and a porous or perforated substrate, a biological membrane, a substrate, a high throughput screen, methods for production of the structure membrane and substrate, and a method for screening a large number of test compounds in a short period. More particularly it relates to a structure comprising a biological membrane adhered to a porous or perforated substrate, a biological membrane capable of adhering with high resistance seals to a substrate such as perforated glass and the ability to form sheets having predominantly an ion channel or transporter of interest, a high throughput screen for determining the effect of test compounds on ion channel or transporter activity, methods for manufacture of the structure, membrane and substrate, and a method for monitoring ion channel or transporter activity in a membrane.Type: GrantFiled: June 23, 2014Date of Patent: June 26, 2018Assignee: SOPHION BIOSCIENCE A/SInventors: David Geraint Owen, Nicholas Gerard Byrne
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Patent number: 9975120Abstract: A chip assembly for use in a microfluidic analysis system, such as a patch clamp apparatus, comprises a chip having an outer wall, a carrier structure comprising an aperture for receiving the chip, the aperture defining an inner wall, wherein the chip is arranged in the aperture with a liquid tight seal between the outer wall of the chip and the inner wall of the aperture. The chip may be sealed and bonded to the carrier structure by means of a bonding material, such as an UV curing adhesive. A through hole in the chip is aligned with the aperture in the carrier structure. A method for manufacturing the chip assembly is further disclosed.Type: GrantFiled: July 25, 2016Date of Patent: May 22, 2018Assignee: SOPHION BIOSCIENCE A/SInventors: Anders Brask, Jonatan Kutchinsky
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Patent number: 9649630Abstract: The invention provides a chip for use in a microfluidic analysis system, for example a patch-clamp system, said chip having improved cell adhesion through a predetermined pattern of hydrophobic and hydrophilic regions. A method for manufacture of the chips, and a method for improving the adhesion of a cell to a chip are also disclosed.Type: GrantFiled: January 8, 2013Date of Patent: May 16, 2017Assignee: Sophion Bioscience A/SInventor: Sandra Wilson
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Patent number: 9581562Abstract: A handheld device for analysis of electrophysiological properties of a cellular membrane ion channel in an ion channel containing lipid cellular membrane includes a handheld body with a pump, an electronic controller, and a disposable pipette tip including a pathway for fluid. The pathway connects an open end of the pipette tip to an analysis substrate in the pipette tip. The substrate is adapted to transmit an electrical current through the ion channel. The handheld body and the disposable pipette tip are configured to releasably attach the pipette tip to the body, to provide a hydraulic connection between the pump and the pathway, and to provide an electric connection between the electronic controller and at least one of the electrodes of the substrate. The electronic controller of the handheld body is configured to operate the assembled device for microfluidic analysis of aspirated fluid.Type: GrantFiled: February 29, 2012Date of Patent: February 28, 2017Assignee: SOPHION BIOSCIENCE A/SInventors: Jonatan Kutchinsky, Nicholas Byrne, Christopher Mathes, Jens Henneke
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Publication number: 20140353171Abstract: The invention provides a chip for use in a microfluidic analysis system, for example a patch-clamp system, said chip having improved cell adhesion through a predetermined pattern of hydrophobic and hydrophilic regions. A method for manufacture of the chips, and a method for improving the adhesion of a cell to a chip are also disclosed.Type: ApplicationFiled: January 8, 2013Publication date: December 4, 2014Applicant: Sophion Bioscience A/SInventor: Sandra Wilson
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Publication number: 20130334062Abstract: A handheld device for analysis of electro-physiological properties of a cellular membrane ion channel in an ion channel containing lipid cellular membrane comprises a handheld body with a pump and an electronic controller, and a disposable pipette tip comprising a pathway for fluid, said pathway connecting an open end of the pipette tip to an analysis substrate comprised in the pipette tip. The substrate is adapted to transmit an electrical current through the ion channel in said ion channel-containing lipid cellular membrane, when said lipid cellular membrane is held at a predetermined site of the substrate, e.g. for patch clamp analysis. The handheld body and the disposable pipette tip are configured to releasably attach the pipette tip to the body, to provide a hydraulic connection between the pump of the handheld body and said pathway, and to provide an electric connection between the electronic controller and at least one of said electrodes of the substrate.Type: ApplicationFiled: February 29, 2012Publication date: December 19, 2013Applicant: SOPHION BIOSCIENCE A/SInventors: Jonatan Kutchinsky, Nicholas Byrne, Christopher Mathes, Jens Henneke
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Publication number: 20130136673Abstract: A chip assembly for use in a microfluidic analysis system, such as a patch clamp apparatus, comprises a chip having an outer wall, a carrier structure comprising an aperture for receiving the chip, the aperture defining an inner wall, wherein the chip is arranged in the aperture with a liquid tight seal between the outer wall of the chip and the inner wall of the aperture. The chip may be sealed and bonded to the carrier structure by means of a bonding material, such as an UV curing adhesive. A through hole in the chip is aligned with the aperture in the carrier structure. A method for manufacturing the chip assembly is further disclosed.Type: ApplicationFiled: July 6, 2011Publication date: May 30, 2013Applicant: SOPHION BIOSCIENCE A/SInventors: Anders Brask, Jonatan Kutchinsky
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Patent number: 8268260Abstract: A microfluidics system comprising a channel having an inlet (32) and an outlet (38); a first membrane (31) positioned between the inlet (32) and outlet (38) and comprising an aperture having a radius within the range 0.1 to 50 ?m, the inlet (32) and the outlet (38) being in hydraulic communication with one another, such that a fluid can move along the channel from the inlet to the outlet.Type: GrantFiled: February 23, 2004Date of Patent: September 18, 2012Assignee: Sophion Bioscience A/SInventors: Rafael Taboryski, Jonatan Kutchinsky
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Publication number: 20080073213Abstract: An electroosmotic flow pump for generating a flow in an electrolyte from an inlet to an outlet in a channel, the electroosmotic flow pump comprising a housing with the channel for holding the ionic solution, a membrane separating the channel in a first part in contact with the inlet and a second part in contact with the outlet, the membrane comprising a plurality of perforations having inner surface parts with a finite zeta potential in an 130-160 mM aqueous electrolyte with pH value in the interval 7-7.5, one or more first electrodes in electrical contact with electrolyte held in the first part of the channel and one or more second electrodes in electrical contact with electrolyte held in the second part of the channel, means for creating an electric potential difference between the first and second electrodes.Type: ApplicationFiled: February 23, 2004Publication date: March 27, 2008Applicant: SOPHION BIOSCIENCE A/SInventors: Rafael Taboryski, Simon Pedersen, Jonatan Kutchinsky, Claus Birger Sorensen
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Patent number: 7322926Abstract: A centrifugal device comprising: a rotor having a rotor shaft having a longitudinal rotor axis; a holder having an access end, and a holder axis; a location mechanism for locating the holder within the centrifugal device, such that the holder is rotatable about the rotor axis, with the holder axis forming a non-zero angle with the rotor shaft axis, the location mechanism accurately locating the access end of the holder within the centrifugal device, wherein the location mechanism comprises a holding pin having an axis that is substantially perpendicular to the rotor axis, the holding pin being rotatable about the rotor axis.Type: GrantFiled: February 23, 2004Date of Patent: January 29, 2008Assignee: Sophion Bioscience A/SInventors: Jorgen Due, Karen-Margrethe Krzywkowski, Nicholas Oswald, John Larsen, Henrik Vennerberg
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Patent number: 7288785Abstract: The present invention relates to a substantially planar substrate for use in patch clamp analysis of the electrophysiological properties of a cell membrane comprising a glycocalyx, wherein the substrate comprises an aperture having a rim, the rim being adapted to form a gigaseal upon contact with the cell membrane, The invention further provides a method of making such a substrate and method for analysing the electrophysiological properties of a cell membrane comprising a glycocalyx.Type: GrantFiled: April 17, 2003Date of Patent: October 30, 2007Assignee: Sophion Bioscience A/SInventors: Ras Kaas Vestergaard, Niels Willumsen, Nicholas Oswald, Jonatan Kutchinsky, Dirk Reuter, Rafael Taboryski
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Publication number: 20060287182Abstract: A centrifugal device comprising: a rotor having a rotor shaft having a longitudinal rotor axis; a holder having an access end, and a holder axis; location means for locating the holder within the centrifugal device, such that the holder is rotatable about the rotor axis, with the holder axis forming a non-zero angle with the rotor shaft axis, the location means accurately locating the access end of the holder within the centrifugal device, wherein the location means comprises a holding pin having an axis that is substantially perpendicular to the rotor axis, the holding pin being rotatable about the rotor axis.Type: ApplicationFiled: February 23, 2004Publication date: December 21, 2006Applicant: SOPHION BIOSCIENCE A/SInventors: Jorgen Due, Karen-Margarethe Krzywkowksi, Nicholas Oswald, John Larsen, Henrik Vennerberg
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Publication number: 20060251544Abstract: A microfluidics system comprising a channel having an inlet (32) and an outlet (38); a first membrane (31) positioned between the inlet (32) and outlet (38) and comprising an aperture having a radius within the range 0.1 to 50 ?m, the inlet (32) and the outlet (38) being in hydraulic communication with one another, such that a fluid can move along the channel from the inlet to the outlet.Type: ApplicationFiled: February 23, 2004Publication date: November 9, 2006Applicant: Sophion Bioscience A/SInventors: Rafael Taboryski, Jonatan Kutchinsky
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Patent number: 7037082Abstract: The present invention provides a pump for generating an Electroosmotic Flow (EOF) in a solution in a canal, guide, pipe or equivalent. Electroosmotic flow is generated by application of an electric field through a solution in a canal defined by insulating walls. The phenomenon depends on ionisation of sites on the surface so that for electroneutrality there is an excess mobile charge in the solution. The electric field acts on the excess charge in the solution causing the fluid to flow. The quantity and distribution of excess charge in the solution depends on the solution and the surface materials and is related to a parameter, the zeta (z) potential characterising material/solution combinations.Type: GrantFiled: October 2, 2002Date of Patent: May 2, 2006Assignee: Sophion Bioscience A/SInventors: Rafael Taboryski, Jonatan Kutchinsky, Morten Bech
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Publication number: 20050212095Abstract: The present invention relates to a substantially planar substrate for use in patch clamp analysis of the electrophysiological properties of a cell membrane comprising a glycocalyx, wherein the substrate comprises an aperture having a rim, the rim being adapted to form a gigaseal upon contact with the cell membrane. The invention further provides a method of making such a substrate and method for analysing the electrophysiological properties of a cell membrane comprising a glycocalyx.Type: ApplicationFiled: April 17, 2003Publication date: September 29, 2005Applicant: Sophion Bioscience A/SInventors: Ras Vestergaard, Niels Willumsen, Nicholas Oswald, Jonatan Kutchinsky, Dirk Reuter, Rafael Taboryski