Abstract: An alcohol soluble protein gas-liquid interface self-assembled porous membrane and a preparation method thereof are provided. The alcohol soluble protein gas-liquid interface self-assembled porous membrane is prepared from an alcohol soluble protein membrane storage solution by an anti-solvent method. The porous interface membrane is rapidly prepared by an alcohol soluble protein interface self-assembled one-step method and can be rapidly formed within 4 seconds, which greatly improves the preparation efficiency of the alcohol soluble protein membrane. The structure (size, pore diameter, micro porosity) of the alcohol soluble protein interface self-assembled porous membrane is precisely regulated and controlled by regulating and controlling process parameters, and a new preparation solution of an alcohol soluble protein base membrane, which is more efficient and has a modifiable structure compared with an alcohol soluble protein membrane prepared by a traditional solvent evaporation method, is developed.

Abstract: Disclosed is a method for producing biogas from a waste liquid/residue after diosgenin extraction by aluminum chloride. Specifically, in a fermentation vessel, an anaerobic sludge and a waste residue from diosgenin extraction by aluminum chloride are added at a volatile solids ratio of (1-3):(1-2), or a seeding sludge and a diosgenin waste liquid are added with an organic loading having a VS:COD ratio of (1-2):(1-2); and then the above components are fermented at 35-37° C. for 5-25 d such that biogas is produced. According to the method of the present invention, the maximum cumulative biogas production rates are 255.4 mL/g VS for waste residue and 326.9 mL/g COD for waste liquid, and throughout the fermentation period, ammoniacal nitrogen values are below 1500 mg/L and pH variation is within the normal range, and the COD removal rate for the fermentation broth is 91.11%.

Abstract: A use of a ZmSBP12 gene in the regulation of drought resistance, plant height, and ear height of Zea mays L. is provided. After the ZmSBP12 gene is over-expressed in Zea mays L., the resulting Zea mays L. mutant plant exhibits increased drought resistance and decreased plant and ear heights. The overexpression of the ZmSBP12 gene leads to increased drought resistance and decreased plant and ear heights, indicating that the ZmSBP12 gene plays a crucial role in the drought resistance and plant type (plant height) of Zea mays L. The expression abundance of the ZmSBP12 gene is increased to improve the drought resistance of Zea mays L. and reduce the plant and ear heights of Zea mays L., which can be used for the assisted breeding of novel drought-resistant and lodging-resistant Zea mays L. varieties and the breeding of excellent inbred lines and hybrids of Zea mays L.

Abstract: A wood-plastic/lumber composite co-extrusion feeder includes a frame, wherein at least one group of toothed conveying units, a tooth mark milling unit for milling tooth marks on an outer surface of a lumber, and a lumber co-extrusion mold are arranged on the frame in sequence, each toothed conveying unit includes a lower toothed pressure roller installed on a first fixed bearing seat and an upper toothed pressure roller installed on a first movable bearing seat, and after the first fixed bearing seat and the first movable bearing seat are connected by an adjustment unit, a first conveying channel having an adjustable height is formed between the upper toothed pressure roller and the lower-toothed pressure roller.

Abstract: Embodiments of the present disclosure belongs to the technical field of animal feed and provides a method for rapidly evaluating metabolizable energy of goose diet by using a technique of simulative digestion gross energy. By using the technical means combining the biological method and the simulative digestion gross energy technique, metabolizable energy of goose feed can be evaluated quickly. Based on the “stomach-small intestine” two-step enzymatic methods, it is the first time to establish a regression equation between the metabolizable energy change and fiber level in the cecum to rectify the value of simulative digestion gross energy in the cecal microbial digestion phase, making the simulative digestion gross energy technique more reasonable in the assessment of metabolizable energy in geese. Results show that the use of simulative digestion gross energy technique to assess the metabolizable energy of goose feed value is highly feasible.

Abstract: The present disclosure relates to the technical field of agricultural microorganisms and crop cultivation and more specifically relates to a compound mycorrhizal fungus growth promoting agent and an agricultural-photovoltaic complementary planting method for Dioscorea composita. The compound mycorrhizal fungus growth promoting agent for Dioscorea composita includes Glomus mosseae, Streptomyces chartreusi and plant growth promoting rhizobacteria, and can greatly improve photosynthesis of Dioscorea composita under a photovoltaic panel and promote growth and development of Dioscorea composita. The compound mycorrhizal fungus growth promoting agent is used, an unoccupied land under a solar photovoltaic panel is utilized, and a high-ridge close planting method is used to combine a solar photovoltaic industry and Dioscorea composita planting. The whole planting process is easy to operate, the cost is low, and three-dimensional value-added utilization of land is achieved.

Abstract: Disclosed is a molecular marker for genetic resistance of chicken to infection by subgroups A and K avian leukosis virus (ALV-A and ALV-K) and use thereof the molecular marker is tva gene with base deletion between 318-323 and/or between 602-607; specifically, bases ACCTCC at positions 318-323 and bases CCGCTG at positions 602-607 are deleted. In the present disclosure, genetic variation of tva receptor gene in Chinese chicken breeds is analyzed, and it is found that the DNA sequence of tva receptor gene in the Chinese chicken breeds has base deletion at positions 318-323 or at positions 602-607. Moreover, a method for breeding of the chicken breeds resistance to ALV-A and ALV-K has been established.

Abstract: An alcohol soluble protein gas-liquid interface self-assembled porous membrane and a preparation method thereof are provided. The alcohol soluble protein gas-liquid interface self-assembled porous membrane is prepared from an alcohol soluble protein membrane storage solution by an anti-solvent method. The porous interface membrane is rapidly prepared by an alcohol soluble protein interface self-assembled one-step method and can be rapidly formed within 4 seconds, which greatly improves the preparation efficiency of the alcohol soluble protein membrane. The structure (size, pore diameter, micro porosity) of the alcohol soluble protein interface self-assembled porous membrane is precisely regulated and controlled by regulating and controlling process parameters, and a new preparation solution of an alcohol soluble protein base membrane, which is more efficient and has a modifiable structure compared with an alcohol soluble protein membrane prepared by a traditional solvent evaporation method, is developed.

Abstract: The present disclosure provides a method for estimating a body size and weight of a pig based on deep learning, and relates to the technical field of deep learning. The present disclosure predicts the weight of the pig by using a convolutional neural network. Relevant features are learned by the convolutional neural network, and feature engineering extraction is not needed to be established, so that extracted features are more comprehensive, and the convolutional neural network is superior to a linear model in processing of noisy data and nonlinear problems of data. Images of the pig are shot by an ordinary two-dimensional (2d) color camera.

Abstract: Provided is a method for upgrading pyrolysis oil through seawater electrochemical pretreatment of biomass and use thereof. The method includes: (1) crushing and sieving a biomass raw material to obtain a crushed biomass raw material, adding the crushed biomass raw material to a salt solution and mixing to be uniform to obtain a reactant mixture; performing an electrolytic reaction on the reactant mixture under conditions of stirring and an external voltage of 5-15 V for 2-8 hours to obtain a product mixture; after the electrolytic reaction, subjecting the product mixture to a suction filtration, collecting a filter cake, washing the filter cake and drying to obtain a pretreated biomass, and (2) subjecting the pretreated biomass obtained in step (1) to a pyrolysis reaction at a temperature of 400-600° C. for 30-90 minutes in a protective gas atmosphere, and collecting a pyrolysis oil by an organic solvent.

Abstract: Disclosed is a method for producing biogas from a waste liquid/residue after diosgenin extraction by aluminum chloride. Specifically, in a fermentation vessel, an anaerobic sludge and a waste residue from diosgenin extraction by aluminum chloride are added at a volatile solids ratio of (1-3):(1-2), or a seeding sludge and a diosgenin waste liquid are added with an organic loading having a VS:COD ratio of (1-2):(1-2); and then the above components are fermented at 35-37° C. for 5-25 d such that biogas is produced. According to the method of the present invention, the maximum cumulative biogas production rates are 255.4 mL/g VS for waste residue and 326.9 mL/g COD for waste liquid, and throughout the fermentation period, ammoniacal nitrogen values are below 1500 mg/L and pH variation is within the normal range, and the COD removal rate for the fermentation broth is 91.11%.

Abstract: A method for counting suckling piglets based on self-attention spatiotemporal feature fusion is disclosed, which includes: detecting a side-lying sow in a video frame by using CenterNet to acquire a key frame of suckling piglets and a region of interest of the video frame, and overcome the interference of the movement of non-suckling piglets on the spatiotemporal feature extraction for the region of interest; transforming spatiotemporal features extracted by a spatiotemporal two-stream convolutional network from a key frame video clip into a spatiotemporal feature vector, inputting the obtained spatiotemporal feature vector into a temporal, a spatial and a fusion transformer to obtain a self-attention matrix; performing element-wise product for the self-attention matrix and the fused spatiotemporal features to obtain a self-attention spatiotemporal feature map; and inputting the self-attention spatiotemporal feature map into a regression branch of the number of suckling piglets to complete the counting of the

Abstract: A use of a ZmSBP12 gene in the regulation of drought resistance, plant height, and ear height of Zea mays L. is provided. After the ZmSBP12 gene is over-expressed in Zea mays L., the resulting Zea mays L. mutant plant exhibits increased drought resistance and decreased plant and ear heights. The overexpression of the ZmSBP12 gene leads to increased drought resistance and decreased plant and ear heights, indicating that the ZmSBP12 gene plays a crucial role in the drought resistance and plant type (plant height) of Zea mays L. The expression abundance of the ZmSBP12 gene is increased to improve the drought resistance of Zea mays L. and reduce the plant and ear heights of Zea mays L., which can be used for the assisted breeding of novel drought-resistant and lodging-resistant Zea mays L. varieties and the breeding of excellent inbred lines and hybrids of Zea mays L.

Abstract: The present disclosure discloses a whole avian-origin reverse genetic manipulation system and its use in producing a recombinant H7N9 avian influenza vaccine. The whole avian-origin reverse genetic manipulation system is an eight-plasmid reverse genetic manipulation system based on H5N2 subtype avian influenza D7 virus strain, which is comprised of 8 recombinant plasmids respectively containing PB2, PB1, PA, HA, NP, NA, M and NS gene fragments derived from H5N2 subtype avian influenza D7 virus strain. The genome of the recombinant H7N9 subtype avian influenza vaccine of the present disclosure is comprised of an NA gene and a modified HA gene derived from a highly pathogenic H7N9 subtype avian influenza virus strain, as well as PB2, PB1, PA, NP, M and NS genes derived from H5N2 subtype avian influenza D7 virus strain.

Abstract: A key DNA sequence for regulating a maize leaf angle and a mutant thereof are provided, which have polynucleotide sequences shown in SEQ ID No. 1 and SEQ ID No. 2, respectively. The DNA sequence for regulating a maize leaf angle and the mutant thereof provided by present disclosure can regulate the expression of ZmNAC16 gene in a maize pulvinus, and thus can be used for the improvement of maize leaf angle and plant type and further for the cultivation of new maize varieties. The present disclosure further provides specific detection primers for detecting mutations of the DNA key sequence and the mutant, and detection primers for detecting an expression level of ZmNAC16 gene in maize. These detection primers can be used to directionally improve a maize leaf angle and also shows application potential for breeding of dense-planting-tolerant and high-yield maize.

Abstract: The present disclosure discloses a whole avian-origin reverse genetic system, a recombinant H5N2 subtype avian influenza virus, a vaccine containing the virus, and a preparation method and application thereof. The genome of the recombinant virus is comprised of a modified HA gene derived from a highly pathogenic H5N6 subtype avian influenza virus strain, as well as PB2, PB1, PA, NP, NA, M and NS genes derived from H5N2 subtype avian influenza D7 virus strain. The recombinant virus is a recombinant H5N2 avian influenza virus rescued from the D7 virus strain as a backbone, which is an avirulent virus strain with the original immunogenicity, and can maintain a high virus titer during the chick embryo culture process. The recombinant virus fully meets the biological safety requirements and has a good application prospect.

Abstract: The present disclosure discloses a whole avian-origin reverse genetic system, a recombinant H5N2 subtype avian influenza virus, a vaccine containing the virus, and a preparation method and application thereof. The genome of the recombinant virus is comprised of a modified HA gene derived from a highly pathogenic H5N6 subtype avian influenza virus strain, as well as PB2, PB1, PA, NP, NA, M and NS genes derived from H5N2 subtype avian influenza D7 virus strain. The recombinant virus is a recombinant H5N2 avian influenza virus rescued from the D7 virus strain as a backbone, which is an avirulent virus strain with the original immunogenicity, and can maintain a high virus titer during the chick embryo culture process. The recombinant virus fully meets the biological safety requirements and has a good application prospect.

Abstract: The present disclosure discloses a whole avian-origin reverse genetic manipulation system and its use in producing a recombinant H7N9 avian influenza vaccine. The whole avian-origin reverse genetic manipulation system is an eight-plasmid reverse genetic manipulation system based on H5N2 subtype avian influenza D7 virus strain, which is comprised of 8 recombinant plasmids respectively containing PB2, PB1, PA, HA, NP, NA, M and NS gene fragments derived from H5N2 subtype avian influenza D7 virus strain. The genome of the recombinant H7N9 subtype avian influenza vaccine of the present disclosure is comprised of an NA gene and a modified HA gene derived from a highly pathogenic H7N9 subtype avian influenza virus strain, as well as PB2, PB1, PA, NP, M and NS genes derived from H5N2 subtype avian influenza D7 virus strain.

Abstract: The present invention relates to a photo-induced cationic polymerized pure vegetable oil-based polymer and a preparation method and use thereof. The preparation method comprises the following steps: mixing a dry oil, an epoxy vegetable oil and an initiator uniformly, irradiating to initiate a photo-curing reaction, and then placing at ambient temperature, and continuing to a heat-curing reaction so as to obtain a photo-induced cationic polymerized pure vegetable oil-based polymer. In the present invention, vegetable oil resources which are low in price, widespread, and easy to regenerate are used to prepare the pure vegetable oil-based polymers instead of the fossil-derived monomers completely, thereby achieving the efficient use of vegetable oils. In the present invention, an unconventional photo-induced heat frontal polymerization technology is used to prepare the pure vegetable oil-based polymer, thereby achieving a photo-thermal dual curing reaction of a vegetable oil system without heating.

Abstract: The present disclosure discloses a Microcin MccY, and a preparation method and use thereof, wherein the amino acid sequence of the Microcin MccY is GGRGHIAEYFSGPITQVSFYG. Compared with Microcin MccJ25 that only has a bactericidal activity against a small part of serotypes of Salmonella such as Salmonella enteritidis, the Microcin MccY has bacteriostatic/bactericidal effects on Salmonella Pullorum, Salmonella typhimurium, Salmonella kentucky, Salmonella Infantis, Salmonella London, and Shigella sonnei, and can kill other serotypes of Salmonella that MccJ25 cannot kill. Especially, the Microcin MccY has an outstanding bactericidal effect on Salmonella typhimurium and Salmonella Pullorum which are common in livestock and poultry production, and meanwhile it also has bacteriostatic/bactericidal effects on Shigella sonnei. Therefore, the Microcin MccY overcomes the shortcoming of the narrow spectrum of the Microcin, has breakthrough significance, and has the potential as a substitute to antibiotics.