Abstract: Methods and compositions related to lipoproteins reactions are provided. Particularly, quantitation of lipoprotein subgroup particle numbers may be used to detect lipoprotein's response to oxidation conditions and incubation conditions. In further aspects, ultracentrifugation may be improved to analyze the lipoprotein profiles. Embodiments are directed to methods for generating a oxidation susceptibility measure for serum lipoproteins. In certain aspects a first portion of a serum sample is used to generate a base lipid particle profile (LPP) and a second portion of the serum sample is used to generate an oxidized lipid particle profile.

Abstract: A new lipoprotein analysis procedure based on the CDC gold standard, analytical ultra centrifugation, having dramatically reduced the time and cost to obtain a result with a self generating continuous gradient. The method allows quantification of the risk factors of cardiovascular disease based on particle numbers of the particles of various groups and subgroups of lipoproteins.