Patents Assigned to SPECTRAL APPLIED RESEARCH INC.
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Total internal reflectance fluorescence (TIRF) microscopy across multiple wavelengths simultaneously
Patent number: 9500847Abstract: A multiple wavelength total internal reflection fluorescence (TIRF) microscopy system has an objective. A dispersion unit of the system comprises a high-dispersion optical element. The dispersion unit receives illumination light having at least a first wavelength ?1 and a second wavelength ?2, where ?1??2, and splits the illumination light into a first monochromatic beam having the first wavelength ?1 and a second monochromatic beam having the second wavelength ?2. The monochromatic beams are focused onto a back focal plane of the objective, near an outer edge of the objective, at different radial distances from an optical axis of the objective. The dispersion unit is rotatable in order to adjust angles of incidence of the monochromatic beams onto an interface between a substrate and a sample to be imaged, wherein the angles of incidence are greater than the critical angle of the interface.Type: GrantFiled: October 14, 2013Date of Patent: November 22, 2016Assignee: Spectral Applied Research Inc.Inventors: Richard Berman, Peter Maclean Sinclair, John Oreopoulos -
Patent number: 8922887Abstract: Where a multimode fiber is used for light delivery in a microscope system and a transverse distribution of light exiting a distal end of the fiber is substantially uniform, the distal end is imaged onto a plane of a sample to be probed by the microscope system, or at a conjugate plane. Alternatively, the distal end is imaged onto a plane sufficiently close to the sample plane or the conjugate plane such that a radiant intensity of light at the sample plane or the conjugate plane is substantially uniform. In the case of a multi-focal confocal microscope system, the distal end of the multimode fiber is imaged onto a plane of a segmented focusing array. Alternatively the distal end is imaged onto a plane sufficiently close to the segmented focusing array plane such that a radiant intensity of the light at the segmented focusing array plane is substantially uniform.Type: GrantFiled: January 16, 2014Date of Patent: December 30, 2014Assignee: Spectral Applied Research Inc.Inventor: David James Frederick Cooper
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Publication number: 20140133017Abstract: Where a multimode fiber is used for light delivery in a microscope system and a transverse distribution of light exiting a distal end of the fiber is substantially uniform, the distal end is imaged onto a plane of a sample to be probed by the microscope system, or at a conjugate plane. Alternatively, the distal end is imaged onto a plane sufficiently close to the sample plane or the conjugate plane such that a radiant intensity of light at the sample plane or the conjugate plane is substantially uniform. In the case of a multi-focal confocal microscope system, the distal end of the multimode fiber is imaged onto a plane of a segmented focusing array. Alternatively the distal end is imaged onto a plane sufficiently close to the segmented focusing array plane such that a radiant intensity of the light at the segmented focusing array plane is substantially uniform.Type: ApplicationFiled: January 16, 2014Publication date: May 15, 2014Applicant: SPECTRAL APPLIED RESEARCH INC.Inventor: David James Frederick Cooper
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Total Internal Reflectance Fluorescence (TIRF) Microscopy Across Multiple Wavelengths Simultaneously
Publication number: 20140104680Abstract: A multiple wavelength total internal reflection fluoresence (TIRF) microscopy system has an objective. A dispersion unit of the system comprises a high-dispersion optical element. The dispersion unit receives illumination light having at least a first wavelength ?1 and a second wavelength ?2, where ?1??2, and splits the illumination light into a first monochromatic beam having the first wavelength ?1 and a second monochromatic beam having the second wavelength ?2. The monochromatic beams are focused onto a back focal plane of the objective, near an outer edge of the objective, at different radial distances from an optical axis of the objective. The dispersion unit is rotatable in order to adjust angles of incidence of the monochromatic beams onto an interface between a substrate and a sample to be imaged, wherein the angles of incidence are greater than the critical angle of the interface.Type: ApplicationFiled: October 14, 2013Publication date: April 17, 2014Applicant: Spectral Applied Research Inc.Inventors: Richard Berman, Peter Maclean Sinclair, John Oreopoulos -
Publication number: 20140104681Abstract: In an episcopic multiplexed confocal scanning microscope system, a spatial filter is placed at a conjugate plane to a back aperture plane at a back aperture of an objective lens. The spatial filter is used to combine excitation light and emission light from a multiplexed confocal microscope module.Type: ApplicationFiled: October 14, 2013Publication date: April 17, 2014Applicant: Spectral Applied Research Inc.Inventor: Richard Berman
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Patent number: 8670178Abstract: Where a multimode fiber is used for light delivery in a microscope system and a transverse distribution of light exiting a distal end of the fiber is substantially uniform, the distal end is imaged onto a plane of a sample to be probed by the microscope system, or at a conjugate plane. Alternatively, the distal end is imaged onto a plane sufficiently close to the sample plane or the conjugate plane such that a radiant intensity of light at the sample plane or the conjugate plane is substantially uniform. In the case of a multi-focal confocal microscope system, the distal end of the multimode fiber is imaged onto a plane of a segmented focusing array. Alternatively the distal end is imaged onto a plane sufficiently close to the segmented focusing array plane such that a radiant intensity of the light at the segmented focusing array plane is substantially uniform.Type: GrantFiled: December 7, 2010Date of Patent: March 11, 2014Assignee: Spectral Applied Research Inc.Inventor: David James Frederick Cooper
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Publication number: 20130010353Abstract: A multi-mode optical fiber delivers light from a radiation source to a multi-focal confocal microscope with reasonable efficiency. A core diameter of the multi-mode fiber is selected such that an etendue of light emitted from the fiber is not substantially greater than a total etendue of light passing through a plurality of pinholes in a pinhole array of the multi-focal confocal microscope. The core diameter may be selected taking into account a specific optical geometry of the multi-focal confocal microscope, including pinhole diameter and focal lengths of relevant optical elements. For coherent radiation sources, phase randomization may be included. A multi-mode fiber enables the use of a variety of radiation sources and wavelengths in a multi-focal confocal microscope, since the coupling of the radiation source to the multi-mode fiber is less sensitive to mechanical and temperature influences than coupling the radiation source to a single mode fiber.Type: ApplicationFiled: September 4, 2012Publication date: January 10, 2013Applicant: SPECTRAL APPLIED RESEARCH INC.Inventor: Richard Berman
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Publication number: 20110134519Abstract: Where a multimode fiber is used for light delivery in a microscope system and a transverse distribution of light exiting a distal end of the fiber is substantially uniform, the distal end is imaged onto a plane of a sample to be probed by the microscope system, or at a conjugate plane. Alternatively, the distal end is imaged onto a plane sufficiently close to the sample plane or the conjugate plane such that a radiant intensity of light at the sample plane or the conjugate plane is substantially uniform. In the case of a multi-focal confocal microscope system, the distal end of the multimode fiber is imaged onto a plane of a segmented focusing array. Alternatively the distal end is imaged onto a plane sufficiently close to the segmented focusing array plane such that a radiant intensity of the light at the segmented focusing array plane is substantially uniform.Type: ApplicationFiled: December 7, 2010Publication date: June 9, 2011Applicant: Spectral Applied Research Inc.Inventor: David James Frederick Cooper
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Publication number: 20100142041Abstract: A multi-mode optical fiber delivers light from a radiation source to a multi-focal confocal microscope with reasonable efficiency. A core diameter of the multi-mode fiber is selected such that an etendue of light emitted from the fiber is not substantially greater than a total etendue of light passing through a plurality of pinholes in a pinhole array of the multi-focal confocal microscope. The core diameter may be selected taking into account a specific optical geometry of the multi-focal confocal microscope, including pinhole diameter and focal lengths of relevant optical elements. For coherent radiation sources, phase randomization may be included. A multi-mode fiber enables the use of a variety of radiation sources and wavelengths in a multi-focal confocal microscope, since the coupling of the radiation source to the multi-mode fiber is less sensitive to mechanical and temperature influences than coupling the radiation source to a single mode fiber.Type: ApplicationFiled: December 7, 2009Publication date: June 10, 2010Applicant: SPECTRAL APPLIED RESEARCH INC.Inventor: Richard Berman