Patents Assigned to Spiber Technologies AB
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Publication number: 20230070786Abstract: A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NOs: 62-65 or 67-73; and a sequence having at least 70% identity to SEQ ID NOs: 64 or any one of SEQ ID NOs: 62-65 or 67-73, wherein the sequence compriseType: ApplicationFiled: August 25, 2022Publication date: March 9, 2023Applicant: SPIBER TECHNOLOGIES ABInventors: Anna RISING, Jan JOHANSSON, Marlene ANDERSSON
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Patent number: 11524984Abstract: A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NOs: 62-65 or 67-73; and a sequence having at least 70% identity to SEQ ID NOs: 64 or any one of SEQ ID NOs: 62-65 or 67-73, wherein the sequence compriseType: GrantFiled: June 29, 2017Date of Patent: December 13, 2022Assignee: SPIBER TECHNOLOGIES ABInventors: Anna Rising, Jan Johansson, Marlene Andersson
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Patent number: 11484624Abstract: A method for coating a solid surface with a recombinant spider silk protein capable of forming polymeric, solid structures is provided. The method is comprising the following steps: exposing the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming a surface layer of the recombinant spider silk protein adsorbed on the solid surface without formation of covalent bonds between the recombinant spider silk protein and the solid surface; and further exposing the surface layer of the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming an assembled silk structure layer of the recombinant spider silk protein on the surface layer; wherein the method does not include drying-in of spider silk protein.Type: GrantFiled: May 16, 2017Date of Patent: November 1, 2022Assignee: SPIBER TECHNOLOGIES ABInventors: My Hedhammar, Linnea Nilebäck
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Patent number: 11214601Abstract: A protein comprising a moiety of 100-160 amino acid residues having at least 70% identity with the N-terminal (NT) fragment of a spider silk protein, wherein the amino acid residue corresponding to position 40 in NT is selected from the group consisting of Lys, Arg and His; and wherein the amino acid residue corresponding to position 65 in NT is selected from the group consisting of Asp and Glu, is useful as a moiety in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide.Type: GrantFiled: March 12, 2020Date of Patent: January 4, 2022Assignee: SPIBER TECHNOLOGIES ABInventors: Jan Johansson, Anna Rising, Nina Kronqvist, Kerstin Nordling
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Publication number: 20200283487Abstract: The present invention provides a fusion protein comprising: i) a solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein; and ii) a C-type lectin polypeptide. The present invention also provides a truncated SP-A polypeptide which lacks the N-terminal domain of full SP-A and is capable of trimerisation, and to the use of such a truncated SP-A polypeptide in treating or preventing a disease.Type: ApplicationFiled: December 21, 2016Publication date: September 10, 2020Applicants: University of Southampton, Spiber Technologies ABInventors: Howard William Clark, Alastair Samuel Watson, Jens Madsen, Jan Johansson, Anna Rising, Nina Kronqvist, Kerstin Nordling
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Publication number: 20200207819Abstract: A protein comprising a moiety of 100-160 amino acid residues having at least 70% identity with the N-terminal (NT) fragment of a spider silk protein, wherein the amino acid residue corresponding to position 40 in NT is selected from the group consisting of Lys, Arg and His; and wherein the amino acid residue corresponding to position 65 in NT is selected from the group consisting of Asp and Glu, is useful as a moiety in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide.Type: ApplicationFiled: March 12, 2020Publication date: July 2, 2020Applicant: SPIBER TECHNOLOGIES ABInventors: Jan JOHANSSON, Anna RISING, Nina KRONQVIST, Kerstin NORDLING
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Patent number: 10662230Abstract: A recombinant fusion protein is comprising a spider silk fragment and a cyclic RGD cell-binding motif with selectivity for integrins, such as for ?5?1 integrins. The fusion protein is useful as a cell scaffold material and for the cultivation of cells displaying integrins on their cell surface.Type: GrantFiled: May 6, 2019Date of Patent: May 26, 2020Assignee: SPIBER TECHNOLOGIES ABInventor: My Hedhammar
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Patent number: 10626152Abstract: A protein comprising a moiety of 100-160 amino acid residues having at least 70% identity with the N-terminal (NT) fragment of a spider silk protein, wherein the amino acid residue corresponding to position 40 in NT is selected from the group consisting of Lys, Arg and His; and wherein the amino acid residue corresponding to position 65 in NT is selected from the group consisting of Asp and Glu, is useful as a moiety in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide.Type: GrantFiled: November 11, 2016Date of Patent: April 21, 2020Assignee: SPIBER TECHNOLOGIES ABInventors: Jan Johansson, Anna Rising, Nina Kronqvist, Kerstin Nordling
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Patent number: 10604550Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.Type: GrantFiled: April 18, 2017Date of Patent: March 31, 2020Assignee: SPIBER TECHNOLOGIES ABInventors: Jan Johansson, Anna Rising, My Hedhammar, Kerstin Nordling
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Publication number: 20190322710Abstract: A recombinant fusion protein is comprising a spider silk fragment and a cyclic RGD cell-binding motif with selectivity for integrins, such as for ?5?1 integrins. The fusion protein is useful as a cell scaffold material and for the cultivation of cells displaying integrins on their cell surface.Type: ApplicationFiled: May 6, 2019Publication date: October 24, 2019Applicant: Spiber Technologies ABInventor: My Hedhammar
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Publication number: 20190248847Abstract: A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NO:s 62-65 or 67-73; and a sequence having at least 70% identity to SEQ.Type: ApplicationFiled: June 29, 2017Publication date: August 15, 2019Applicant: SPIBER TECHNOLOGIES ABInventors: Anna Rising, Jan Johansson, Marlene Andersson
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Patent number: 10316069Abstract: A recombinant fusion protein is comprising a spider silk fragment and a cyclic RGD cell-binding motif with selectivity for integrins, such as for ?5?1 integrins. The fusion protein is useful as a cell scaffold material and for the cultivation of cells displaying integrins on their cell surface.Type: GrantFiled: June 23, 2016Date of Patent: June 11, 2019Assignee: SPIBER TECHNOLOGIES ABInventor: My Hedhammar
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Publication number: 20190151505Abstract: A method for coating a solid surface with a recombinant spider silk protein capable of forming polymeric, solid structures is provided. The method is comprising the following steps: exposing the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming a surface layer of the recombinant spider silk protein adsorbed on the solid surface without formation of covalent bonds between the recombinant spider silk protein and the solid surface; and further exposing the surface layer of the solid surface to an aqueous solution of the recombinant spider silk protein and thereby forming an assembled silk structure layer of the recombinant spider silk protein on the surface layer; wherein the method does not include drying-in of spider silk protein.Type: ApplicationFiled: May 16, 2017Publication date: May 23, 2019Applicant: SPIBER TECHNOLOGIES ABInventors: My HEDHAMMAR, Linnea NILEBÄCK
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Publication number: 20190144819Abstract: A cell scaffold material is manufactured by providing an aqueous solution of a silk protein capable of assembling into a water-insoluble macrostructure. The silk protein is mixed with eukaryotic cells, and the silk protein is assembled into a water-insoluble macrostructure in the presence of the cells, thereby forming a scaffold material for cultivating the cells. The cells can be grown integrated with the scaffold material under conditions suitable for cell culture.Type: ApplicationFiled: February 10, 2017Publication date: May 16, 2019Applicant: SPIBER TECHNOLOGIES ABInventors: My HEDHAMMAR, Mona WIDHE, Ulrika JOHANSSON
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Publication number: 20180273590Abstract: A protein comprising a moiety of 100-160 amino acid residues having at least 70% identity with the N-terminal (NT) fragment of a spider silk protein, wherein the amino acid residue corresponding to position 40 in NT is selected from the group consisting of Lys, Arg and His; and wherein the amino acid residue corresponding to position 65 in NT is selected from the group consisting of Asp and Glu, is useful as a moiety in a fusion protein for enhancing the solubility of another moiety in the fusion protein, which is a desired protein or polypeptide.Type: ApplicationFiled: November 11, 2016Publication date: September 27, 2018Applicant: SPIBER TECHNOLOGIES ABInventors: Jan JOHANSSON, Anna RISING, Nina KRONQVIST, Kerstin NORDLING
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Publication number: 20180170977Abstract: A recombinant fusion protein is comprising a spider silk fragment and a cyclic RGD cell-binding motif with selectivity for integrins, such as for ?5?1 integrins. The fusion protein is useful as a cell scaffold material and for the cultivation of cells displaying integrins on their cell surface.Type: ApplicationFiled: June 23, 2016Publication date: June 21, 2018Applicant: Spiber Technologies ABInventor: My Hedhammar
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Patent number: 9856308Abstract: A recombinant fusion protein comprising the moieties Band CT, and optionally REP, wherein B is comprising at least one immunoglobulin fragment, which provides the capacity of selective interaction with an organic target; CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein; and REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein.Type: GrantFiled: May 2, 2013Date of Patent: January 2, 2018Assignee: SPIBER TECHNOLOGIES ABInventors: My Hedhammar, Jan Johansson, Anna Rising, Per Åke Nygren
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Publication number: 20170283474Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.Type: ApplicationFiled: April 18, 2017Publication date: October 5, 2017Applicant: SPIBER TECHNOLOGIES ABInventors: Jan JOHANSSON, Anna RISING, My HEDHAMMAR, Kerstin NORDLING
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Patent number: 9708376Abstract: A recombinant fusion protein comprising the moieties B and CT is provided. B is a non-spidroin moiety which provides the capacity of selective interaction with an organic target. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. The fusion protein is not comprising any moiety derived from the repetitive fragment of a spider silk protein.Type: GrantFiled: May 2, 2013Date of Patent: July 18, 2017Assignee: SPIBER TECHNOLOGIES ABInventor: My Hedhammar
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Patent number: 9644012Abstract: A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.Type: GrantFiled: October 27, 2010Date of Patent: May 9, 2017Assignee: SPIBER TECHNOLOGIES ABInventors: Jan Johansson, Anna Rising, My Hedhammar, Kerstin Nordling