Abstract: The present disclosure provides a method for treating a subject with periorbital puffiness by determining if the periorbital puffiness is due to edema or a structural change and administering a protein having hyaluronidase activity to the periorbital region of the subject if the periorbital puffiness is due to edema. Also provided are methods for determining if periorbital puffiness is due to edema or a structural change in a subject in need thereof by administering a composition that comprises a protein having hyaluronidase activity to the periorbital region of the subject, assessing the periorbital region at a predetermined amount of time after the administration of the composition, and then determining whether there is an improvement in the periorbital puffiness.

Abstract: The present disclosure provides methods for determining an etiology of upper and/or lower eyelid puffiness by examining a subject with squinted eyes; and determining if upper and/or lower eyelid puffiness does not improve, improves, partially improves, or worsens, wherein the etiology of the upper and/or lower eyelid puffiness is diagnosed to be anterior to the orbicularis oculi muscle if the puffiness does not improve, wherein the etiology of the upper and/or lower eyelid puffiness is determined to be posterior to the orbicularis oculi muscle if the puffiness improves, wherein the etiology of the upper and/or lower eyelid puffiness is determined to be anterior and posterior to the orbicularis oculi muscle if the puffiness partially improves, or wherein the puffiness is determined to be secondary to hypertrophy of the orbicularis muscle or if the puffiness worsens.

Abstract: The present disclosure provides a method of assessing periorbital puffiness in a subject by determining if the subject exhibits pseudoherniation of one or more upper eyelid fat pads and/or one or more of the lower eyelid fat pads, determining if the subject exhibits one or more conditions selected from the group consisting of: edema of the fat pad, edema along the soft tissue of the upper and/or lower eyelids, malar mounds due to edema, malar edema, and mild festoons aggravated by edema, and scoring the periorbital puffiness from both determinations.

Abstract: The present disclosure provides a method of treating periorbital puffiness in a subject in need thereof by administering a composition that comprises a protein having hyaluronidase activity to a periorbital region of the subject. Also provided is a method of assessing periorbital puffiness in a subject by determining if the subject exhibits pseudoherniation of one or more upper eyelid fat pads and one or more lower eyelid fat pads; determining if the subject exhibits edema in one or more of the upper eyelid fat pads and the one or more of the lower eyelid fat pads; determining if the subject exhibits edema in the periorbital tissue; and scoring the periorbital puffiness due to periorbital edema from each of the prior three determinations.