Abstract: Recombinant laminin B1k and fragments thereof.

Abstract: The present invention is directed toward the human D.sub.4 dopamine receptor. The nucleotide sequence of the gene corresponding to this receptor is provided by the invention. The invention also includes a recombinant eukaryotic expression vector capable of expressing the human D.sub.4 dopamine receptor in cultures of transformed eukaryotic cells and such cultures of transformed eukaryotic cells which synthesize the human D.sub.4 dopamine receptor.

Abstract: Methods for removing ferric iron from aqueous liquids and for performing deferration therapy are disclosed, involving the use of a novel siderophore, termed anguibactin. Anguibactin is isolated from a marine pathogen, Vibrio anguillarum, containing the pMJ1 plasmid. Anguibactin inhibits iron uptake by living cells, wrests iron from vertebrate tissues, removes iron from other siderophores and ferric hydroxide, and removes ferric iron from aqueous solutions, including cell-culture media. For deferration therapy, anguibactin from which bound iron has been removed is administered by dissolving in water or other liquid aqueous pharmaceutical carrier at a dosage typical for other siderophores. Anguibactin is preferably administered intramuscularly or subcutaneously, but can be given intravenously.

Abstract: A purified protein kalinin is disclosed that provides adhesion between epidermal keratinocytes and the underlying dermis. Purified kalinin localizes to the anchoring filaments of basement membranes or human subepithelial skin, trachea, esophagus, cornea and amnion when such areas are probed with BM165 monoclonal antibody after localization. The protein has a molecular weight of approximately 400-460 kDa and exists in a cell-associated form (about 460 kDa) and two medium-associated forms (about 440 and 400 kDa, respectively). The cell-associated form comprises a 200-, a 155- and a 140-kDa subunit, all normally held together by disulflde bonds. The cell-associated form is subjected to extracellular processing to produce the two medium-associated forms, wherein, in the 440-kDa form, the 200-kDa subunit has been processed to a 165-kDa subunit and, in the 400-kDa form, the 155-kDa subunit has been processed to a 105-KDa subunit. The BM165 epitope is located on the 165-kDa subunit.

Abstract: A simple device functioning as a radiorespirometer is a petri dish with a modified cover for quantitating an amount of CO.sub.2 evolved by living cultured cells. The device is a plastic culture dish having a culture surface circumscribed by a continuous sidewall. A removable flat plastic cover has a flat inside face and an annular collar around the face that fits against a sidewall. The collar is maintained in substantially gas-tight relationship against the sidewall by an elastic band or tightly mating surfaces. An opening through the cover is sealed by a gas-impervious material, such as a plastic film, that can be penetrated by a needle to introduce acid into the dish for terminating culture growth and lysing the cells. A collector tube is removably secured to the inside face of the cover and contains a CO.sub.2 trapping material, such as hyamine (methylbenzethonium hydroxide). The culture medium is provided with a cell substrate that contains radioactive carbon, and radioactive CO.sub.

Abstract: Disclosed herein is purified isolated angiogenic factor, isolated from Live Yeast Cell Derivative. Also disclosed herein are methods to treat mammals suffering from wounds or burns comprising administering the angiogenic factor and pharmaceutical formulations for use in the methods.

Abstract: We disclose the discovery and purification of a tumor antigen that binds the mouse monoclonal antibody L6. Immunohistochemical analysis showed that the antigen is expressed on the membrane surface of LX-1 human lung tumor cells, which retain L6 binding activity when intracerebrally xenografted in nude rats. The antigen is also expressed in the cytoplasm of hypothalamic neurons. Inability of oxytocin and vasopressin hormones to block L6 binding showed that the antigenic epitope resides in neurophysin, the carrier protein associated with the two hormones. Porcine neurophysin did block L6 binding to the antigen. Western blot analysis confirmed that L6-immunoreactivity is neurophysin-related. Immunoaffinity chromatography and gel electrophoresis revealed the antigen molecular weight to be 45000 daltons. Amino-terminal sequencing revealed a 21-amino acid homology with the N-terminus of human pro-pressophysin.

Abstract: A method for the delivery of therapeutic agents across the blood brain barrier is disclosed which utilizes drug neutralization technology, and the selective permeability oThis invention was made with support by the Preuss Foundation and with Government support by the Veterans Administration and under Grant No. 31770 awarded by the National Institutes of Health. The Government has certain rights in this invention.

Abstract: Cardiac stimulation signals are routed through a switching network to select only one cardiac stimulation signal as an output signal. The selected output signal may be routed to one of two patient electrode pairs for application to specific stimulation sites on the heart.

Abstract: A DNA probe p79-2-23 is homologous to at least a portion of a hypervariable DNA region located on chromosome 16q22.fwdarw.q24 in the human genome. The DNA region displays a restriction fragment length polymorphism when digested with certain restriction endonucleases. Unrelated individuals display unique fragment patterns on Southern blots probed with p79-2-23. The probe can be used to produce a genetic "fingerprint" for establishing human identity, determining engraftment of bone marrow transplants, determining parentage, and otherwise mapping genes.

Abstract: A DNA probe which is homologous to at least a portion of a hypervariable DNA region D2S3 located at chromosome 2q35-37 in the human genome. The DNA region displays restriction fragment length polymorphism when digested with certain restriction endonucleases. The probe, p5-1-25, can be used to produce a genetic "fingerprint" to establish human identity, determine engraftment of bone marrow transplants, determine parentage, and otherwise map genes.

Abstract: A device and method for calibration of pulse oximeters is disclosed. A standard specimen that has optical characteristics of hemoglobin of a known oxygen saturation is placed in the light path of a pulse oximeter probe. The image of a pulse is created by a filter that periodically increases and decreases the amount of light allowed to reach the sensor of the probe. In one embodiment, the filter comprises a pair of polarizing filters rotated with respect to one another under the control of a digital computer with a motor interface device. In another embodiment, a wedge of a light-attenuating medium, such as a cuvette containing oxygenated blood, is reciprocated in the light path, the wide portions of the wedge attenuating a greater amount of light than the narrow portions. Such a cuvette can include filler openings, with outwardly extending tubes at both the wide and narrow ends.

Abstract: A device and method for calibration of pulse oximeters is disclosed. A standard specimen that has optical charactertistics of hemoglobin of a known oxygen saturation is placed in the light path of a pulse oximeter probe. The image of a pulse is also created by providing a filter that periodically increases and decreases the amount of light allowed to reach the sensor of the probe. In one embodiment, the filter can comprise a pair of polarizing filters rotated with respect to one another. In another embodiment, a wedge of a filtering medium, such as oxygenated blood, is reciprocated in the light path, the wide portions of the wedge attenuating a greater amount of light than the narrow portions.