Abstract: Some aspects of the disclosure are related to systems and methods for controlled oxygen release from biomaterials in vessels and unit operations or components of cell culture, cell containment, and/or bioreactor. Vessels, unit operations, devices, and/or components of the invention may be used to perform all or part of a biological and/or chemical process involving biologicals (e.g., a plurality of cells) in the presence of oxygen-releasing agents. In some embodiments, a system comprises a vessel comprises an oxygen-releasing agent configured to generate in-situ and release oxygen in a sustained manner. The presence of an oxygen-releasing agent may advantageously allow for high cell density fermentation and cell cultivation in a vessel and provide an alternative for supplemental gassing means (e.g., sparger, etc.). Some embodiments of the disclosure are directed to employing the oxygen-releasing agents in microfluidic or millifluidic systems.

Abstract: Containers including internal mixers and actuators assemblies and related methods are generally described. In some embodiments, a container may include a mixer and an actuator, at least one of which may be located inside of the container. The actuator may actuate the mixer to induce flow within the container and mix the contents of the container with a low volumetric footprint and high mixing efficiency. In some embodiments, the actuator may be configured to deform the mixer, which may include one or more features which may be deformed out of plane in a Kirigami or Origami fashion. The actuator and mixer may be arranged in series or in parallel. The actuator may be used without a mixer to induce flow within the container. The actuator may be driven pneumatically, hydraulically, electrically, and/or in any other suitable manner.

Abstract: A storage container for aseptically storing and freezing a biopharmaceutical composition includes a frame, an expandable bladder, and a sensor system. The expandable bladder is supported within the frame having a filled state in which the expandable bladder is configured to hold the biopharmaceutical composition therein. The sensor system is secured to an exterior surface of the bladder. The sensor system is configured to transmit a signal indicative of a temperature of the exterior surface of the bladder which is correlated to a temperature of biopharmaceutical composition at a desired location within the expandable bladder in the filled state thereof.

Abstract: The invention relates to a continuous flow centrifuge with a rotor. The continuous flow centrifuge comprises a connecting section with connection conduits. Via the connection conduits an exchange of media in receptacles arranged in centrifugation chambers of the rotor is possible. A temperature control supply conduit and temperature control discharge conduit for a connecting section temperature control fluid both extend through the connecting section in order to counteract heating of the connecting section as a result of the flexing work and friction occurring therein. The connecting section temperature control fluid flows through the temperature control supply conduit and the temperature control discharge conduit in opposite flow directions. An electronic control unit (28) comprises control logic which controls (open loop or closed loop) the connecting section temperature control circuit.

Abstract: System for detecting at least one presence of foam of a medium in a bioreactor plant, wherein the system comprises: —a bioreactor plant having at least one disposable container for receiving the medium that may comprise the foam; and—at least two capacitive sensor units which are attached at at least two different situating positions of the bioreactor plant, wherein the capacitive sensor units each comprise at least one electrode system for capacitive measurement and are able to detect the presence of foam at the at least two situating positions on the basis of the capacitive measurement; and wherein the capacitive sensor units are designed to transmit captured data relating to the presence of foam to at least one monitoring unit for monitoring, open-loop and/or closed-loop control of foam formation in the bioreactor plant on the basis of said data.

Abstract: The invention relates to a mechanically stable ultrafiltration membrane and to a method for producing such an ultrafiltration membrane.

Abstract: A filtering system (10) includes a filtering apparatus having a filter unit for receiving liquid to be filtered, a receptacle for receiving filtered liquid, and an adapter (14) for coupling the filter unit to the receptacle, and also includes a base (12) having a cradle (26) for receiving the filtering apparatus and a base vacuum delivery port (28). The adapter includes an adapter vacuum receiving port (22) coupled to the vacuum delivery port for driving the liquid through the filter and—as filtered liquid—into the receptacle. The cradle and the adapter have an interlocking mechanism (52, 54) that locks the filtering apparatus to the cradle in a locking position in which the vacuum receiving port is sealingly coupled to the base vacuum delivery port. The base vacuum delivery port has a vacuum valve (30) constituted as a normally-closed valve automatically forced open when the filtering apparatus locks to the cradle.

Abstract: Initially heterogenous biopharmaceutical composition is mixed in a bag designed as a mixed bioreactor with capacity of at least 1500 liters, using an apparatus that includes a tank, the flexible and collapsible bag received in the tank and a stirring device. The device includes an impeller driven from below for initiating agitation adjacent to a tank base. Once the bag is stretched under liquid pressure, it becomes parallelepiped with a bottom wall, a top wall and a sidewall. The bag, in contact against four side panels of the tank, has a rectangular cross section including four rounded corners each spaced from the side wall.

Abstract: The invention relates to a harvesting device (100, 100a, 100b, 100c, 100d) for harvesting a content of a bioreactor bag (102) and to a method for harvesting a content of a bioreactor bag (102). The harvesting device (100, 100a, 100b, 100c, 100d) comprises: a first fixing device (104) for fixing one of the fixing portions (112, 114) to the harvesting device (100, 100a, 100b, 100c, 100d), a second fixing device (106) for fixing the other of the fixing portions (112, 114) to the harvesting device (100, 100a, 100b, 100c, 100d), a base (108), and an adjusting device (110) coupling the first fixing device (104) and/or the second fixing device (106) to the base (108). The adjusting device (110) is configured to selectively allow changing an arrangement of the first fixing portion (112) and/or the second fixing portion (114) relative to the base (108) or fixing the arrangement of the first fixing portion (112) and/or the second fixing portion (114) relative to the base (108).

Abstract: Systems and methods for characterizing a physical and solution properties of liquids are described. In some embodiments, an ultrasonic interrogation signal may be emitted into a liquid such as a solubilized protein solution. A resulting ultrasonic spectrum may be sensed and provided to a trained statistical model of the solution. The trained statistical model may then determine one or more properties of the liquid. In some embodiments, the trained statistical model determines a viscosity of the liquid and/or a solubilized protein concentration of the liquid. In some embodiments, a trained statistical classification model configured to classify the liquid based, for example, on its contents or properties is used to improve modeling accuracy.

Abstract: Valves, systems, articles, and methods for controlling fluid flow are generally described. According to some aspects, valves that can move between an open configuration and a closed configuration in response to a change in magnetic field and/or voltage potential are provided. According to certain aspects, valves comprising elastic diaphragms comprising magnetic materials (e.g., an elastic composite material including magnetic nanoparticles or other magnetic materials dispersed in a matrix) and/or electroactive polymers are provided. In some aspects, magnetically actuated systems and/or voltage potential actuated systems comprising elastomeric valves are described. In some embodiments, valves and systems described herein are useful for liquid handling, dosing, and regulation of fluid flow, though embodiments in which the disclosed valves are used in different applications are also contemplated.

Abstract: The invention relates to a centrifuge, in particular a continuous flow centrifuge, a biotechnical centrifuge or a blood centrifuge. The centrifuge comprises a refrigerant circuit. According to the invention, the outlet side of a controllable compressor is connected to an inlet side of an evaporator via a bypass line with a valve arranged therein. In a normal operation mode, a centrifuge vessel is cooled exclusively via the control of the compressor and an expansion unit while the valve is closed. If, on the other hand, a tolerance range of the target temperature in the centrifuge vessel is left, the valve is opened in order to bring about heating and thus a return of the temperature in the centrifuge vessel to the tolerance range.

Abstract: Various embodiments provide a connector for the sterile transfer of a liquid medium from a container to a bioprocess engineering system. The connector has two coupling devices. The coupling devices each have a housing and a control element which is adjustably mounted in the housing interior and forms a channel. The channel extends from a control element inlet opening to a control element outlet opening. The housings are able to be mechanically connected to one another and have a fluid inlet and a fluid outlet. The fluid outlet of one housing and the fluid inlet of the other housing overlap. The control elements are each adjustable from a starting position, into an operating position, in which the control element inlet opening is fluidically connected to the fluid inlet and the control element outlet opening is fluidically connected to the fluid outlet.

Abstract: A bioreactor having a filter unit and a method for treating a cell broth. The filter unit has a supply channel (2), a first filter medium (4), a retentate channel (1), a second filter medium (5) and a permeate channel (3), arranged so that the first filter medium delimits the supply channel and the retentate channel from one another; and the second filter medium delimits the retentate channel and the permeate channel from one another. The supply channel is connected to an inlet for a supply medium; the retentate channel is connected to an inlet for a cell broth and to an outlet for the cell broth; the permeate channel is connected to an outlet for a permeate; and the interior of the bioreactor is connected to the inlet for the cell broth and to the outlet for the cell broth.

Abstract: A tube holding apparatus includes a body, a mount, a first guide, and a second guide. The body has a proximal end portion and a distal end portion. The mount is positioned between the proximal end portion and the distal end portion of the body. The mount is configured to secure the body to a valve tower. The first guide is secured about the proximal end portion of the body. The second guide is secured about the distal end portion of the body. The first guide and the second guide each configured to receive a portion of a transfer link that extends between a valve tower and a storage container such that a positive slope container to the valve tower.

Abstract: A fluid distribution system includes an input tube, a plurality of vessels, and a distribution hub. The input tube is configured to extend from a supply vessel. Each vessel of the plurality of vessels includes an inflow conduit. The distribution hub includes a single inlet and a plurality of outlets. The single inlet is defined in a bottom of the distribution hub and is in fluid communication with the input tube such that the distribution hub is configured to receive fluid from the input tube through the single inlet. Each outlet of the plurality of outlets is in fluid communication with the single inlet and in fluid communication with a respective inflow conduit such that the distribution hub is configured to provide an equal portion of the fluid received through the single inlet to each of the inflow conduits.

Abstract: Method for open-loop or closed-loop control of a process, in particular a downstream bioprocess, based on the projection of an unknown concentration of at least one substance in a sample using spectroscopy, in particular UV/vis spectroscopy, comprising the steps: Detect spectrums of a plurality of concentration samples, wherein at least two concentration samples have differing concentrations of the substance; generate several quantitative models based on the spectrums of the concentration samples, wherein the models each have a mapping of at least one spectral measurand of the spectrums to concentrations in concentration ranges, wherein the concentration ranges of two models are not identical; detect at least one sample spectrum of the sample; map the sample spectrum to at least one quantitative model of the generated quantitative models; apply the at least one quantitative model that was mapped to the sample spectrum against the sample spectrum to determine a projected value for the unknown concentration; an

Abstract: A thermal capacitor includes a shell and a PCM. The shell includes a first major surface that is configured to contact a container including media to be frozen. The shell defines a cavity in which the PCM is disposed. The PCM has a transition temperature in a range of ?80 degrees Celsius to ?50 degrees Celsius and is configured to rapidly freeze media from room temperature to ?60 degrees Celsius with the container including the media in contact with the shell in an enclosed space.

Abstract: The present invention relates to a chromatography method, a method of determining the concentration of at least one compound in a chromatography method, a method of obtaining an adsorption isotherm, a method of obtaining at least one stationary phase and a method of evaluating the accuracy of a predetermined adsorption isotherm.

Abstract: An organizer assembly including a belt and a ribbon. The ribbon has a first clamp member, a second clamp member, and a first carrier portion. The first clamp member is configured to receive a conduit or a support to fix the conduit or the support relative to the ribbon. The belt includes an elongated body that is slidably received in the first carrier portion. The organizer assembly may include one or more adapters that are received in a respective clamp member of the ribbon and configured to receive a conduit or a support to fix the conduit or support relative to the ribbon.