Abstract: A method of manufacturing a stem cell sheets is provided which includes: (a) obtaining mesenchymal stem cells; (b) extracting programmed death ligands one (PD-L1) from the mesenchymal stem cells; (c) selecting only PD-L1 positive (PD-L1+ MSCs) from the PD-L1; (d) differentiating the PD-L1+ cells into osteoblasts and chondroblasts in a predetermined activation condition; and (e) forming the stem cell sheets by mixing the PD-L1+ MSCs with platelet rich plasma solution and CaCl2).

Abstract: Disclosed is a method of manufacturing a medium composition for cell culture, and a method of manufacturing a crushed stem cell extract using a method of manufacturing a medium composition for cell culture and a 3-low extracting method of active ingredients of a stem cell. The medium composition for cell culture includes a basal medium; a hyaluronic acid; and an additive composition. According to an embodiment, when active ingredients of a stem cell are extracted, a stem cell is crushed at a 3-low circumstance of low temperature, low pressure, a hypotonic circumstance.