Abstract: The present disclosure provides an automated system for processing of tissue. The system comprises a plurality of containers for storing at least one of tissue samples, buffer solutions, enzymes and other reagents, tissue processing container for processing of the tissue, and a robotic assembly coupled to the tissue processing container. The robotic assembly is configured to: carry the tissue processing container towards each of the plurality of containers, and align an inlet port of the tissue processing container with an outlet port of each of the plurality of containers for collecting the liquids, and moves the tissue processing container in multiple-planes to perform at least one of the washing processes, digestion process, phase separation process and combination thereof. The system also comprises of a control unit interfaced with the robotic assembly for controlling operations of the robotic assembly while processing the tissue.

Abstract: The present disclosure provides an automated system for processing of tissue. The system comprises a plurality of containers for storing at least one of tissue samples, buffer solutions, enzymes and other reagents, tissue processing container for processing of the tissue, and a robotic assembly coupled to the tissue processing container. The robotic assembly is configured to: carry the tissue processing container towards each of the plurality of containers, and align an inlet port of the tissue processing container with an outlet port of each of the plurality of containers for collecting the liquids, and moves the tissue processing container in multiple-planes to perform at least one of the washing processes, digestion process, phase separation process and combination thereof. The system also comprises of a control unit interfaced with the robotic assembly for controlling operations of the robotic assembly while processing the tissue.

Abstract: The present disclosure provides a multi plane mixer and separator (MPMS) system. The system comprises a base frame of predetermined shape configured to form a base for the MPMS system. A motor is mounted to the base frame for rotating the MPMS system. A ball joint mechanism is fixed to the motor using a link and other end of the ball joint mechanism is coupled to a fork. A container holding frame is connected to the fork using bars, wherein said container holding frame is capable of tilting up to 120° with respect to the base frame. And an MPMS container of predetermined shape detachably mounted on the container holding frame for mixing fluids of different density.

Abstract: The present disclosure provides a multi plane mixer and separator (MPMS) system. The system comprises a base frame of predetermined shape configured to form a base for the MPMS system. A motor is mounted to the base frame for rotating the MPMS system. A ball joint mechanism is fixed to the motor using a link and other end of the ball joint mechanism is coupled to a fork. A container holding frame is connected to the fork using bars, wherein said container holding frame is capable of tilting up to 120° with respect to the base frame. And an MPMS container of predetermined shape detachably mounted on the container holding frame for mixing fluids of different density.

Abstract: The present disclosure provides an automated system for isolating stromal vascular fraction cells from the mammalian tissue. The system comprises a plurality of containers for storing buffer solutions, tissue samples and digestive buffers. A tissue processing unit fluidly connected to the containers for processing the tissues. The tissue processing unit performs at least one of washing process, digestion process, phase separation process and combination thereof for separating an aqueous fraction of tissue and a fatty fraction. A cell concentration unit fluidly connected to the tissue processing unit for receiving the aqueous fraction of tissue from the tissue processing unit. The cell concentration unit filters the aqueous fraction of tissue by vibrating a filtration assembly by a filter vibrator. A waste collection unit fluidly connectable to the tissue processing unit and cell concentration unit is provided for receiving waste tissues.

Abstract: The present invention relates to gold nanoparticle (GNP) and a method to perpetuate stemness of stem cells comprising step of growing the stem cells in presence of gold particle or Swarna Bhasma. It also relates to compositions for perpetuating stemness of stem cells.

Abstract: The present invention relates to the field of stem cells particularly development of a novel human embryonic model using human embryoid bodies obtained from the human embryonic stem cell. The novel human embryonic model disclosed thus can provide a screening assay for determining the toxic activity of the compound and/or drug.

Abstract: The present invention provides a process of isolation, proliferation and/or maintenance of mesenchymal stem cells (MSCs). The invention further provides a culture medium for proliferation and/or maintenance of human mesenchymal stem cells in xeno-free conditions. The culture medium provided in the present invention proliferates and/or maintains mesenchymal stem cell expansion while maintaining a multipotent phenotype.

Abstract: The present disclosure provides novel culture system and methods for culturing and propagating hESCs in a substantially undifferentiated state for several passages. The ability to grow such cells without differentiation has important applications for therapeutic uses of ES cells for treating human disorders using tissue transplantation and/or gene therapy techniques. In particular, the disclosure further relates to conditioned medium obtained from human germ lineage derived feeder cells (GLDF). The hESC lines are derived, cultured and propagated in substantially undifferentiated state using the conditioned media from GLDF cells of the disclosure. In particular, the disclosure relates to the xeno-free derivation, culture and propagation of hESCs using conditioned medium of GLDF cells obtained thereof.

Abstract: The present disclosure relates to human germ layer derived feeder cells (GLDF cells) and method of generation thereof. Further, it relates to a method for culturing and propagating human embryonic stem cells (hESCs) in a substantially undifferentiated state for several passages on the human GLDF cells. In particular, the present disclosure relates to human GLDF cells which are capable of supporting proliferation of hESCs in a substantially undifferentiated and pluripotent state for several passages.

Abstract: The present invention relates to a method for obtaining master adult pluripotent stem (MAPS) cells from adult human corneal epithelial tissues. The MAPS cells are obtained on the basis of pluripotent markers. Further the invention provides MAPS cells that are capable of self renewal and differentiation and have characteristics similar to that of human embryonic stem cells. The MAPS cells also retain the ability to differentiate into cells of different lineages. The composition comprising MAPS cells are useful for therapeutic purposes. Further, the invention provides a culture medium for proliferation of MAPS cells.