Abstract: The invention provides methods for the detection of prion diseases, such as scrapie of sheep, bovine spongiform encephalopathy of cattle, Creutzfeld-Jacob disease of man, whereby aberrant proteins or prion proteins are detected in tissues which can be sampled from live animals.
Type:
Grant
Filed:
September 24, 2004
Date of Patent:
May 1, 2007
Assignee:
Stichting Instituut Voor Dierhouderij en Diergezondheid
Inventors:
Bram Edward Cornelis Schreuder, Lucius Johannes Mattheus Van Keulen, Maria Elisabeth Wilhelmina Vromans, Johannes Pieter Maria Langeveld, Marinus Adrianus Smits
Abstract: The invention provides methods for the detection of prion diseases, such as scrapie of sheep, bovine spongiform encephalopathy of cattle, Creutzfeld-Jacob disease of man, whereby aberrant proteins or prion proteins are detected in tissues which can be sampled from line animals.
Type:
Grant
Filed:
April 2, 1997
Date of Patent:
December 6, 2005
Assignee:
Stichting Instituut voor Dierhouderij en Diergezondheid
Inventors:
Bram Edward Cornelis Schreuder, Lucius Johannes Mattheus Van Keulen, Maria Elisabeth Wilhelmina Vromans, Johannes Pieter Maria Langeveld, Marinus Adrianus Smits
Abstract: The invention relates to a method of increasing protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1×105 to 5×106 cells/ml with an m.o.i of <0.01. The invention also provides a method to produce recombinant pestivirus E2 or Em9 protein or fragments thereof in insect cell culture characterized by a final concentration of the protein fragments in the growth medium at harvest of at least 100 ?g/ml. The invention also provides a method of producing recombinant FSH, ?-units and/or ?-units, and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 ?/ml.
Type:
Grant
Filed:
February 27, 2003
Date of Patent:
July 19, 2005
Assignees:
Stichting Instituut voor Dierhouderij en Diergezondheid, Bayer AG
Inventors:
Dietmar Kretzdorn, Dirk Franciscus Marinus van de Wiel, Abraham Johannes de Smit, Robertus Jacobus Maria Moormann, Erik Kees Hamann
Abstract: The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1×105 to 5×106 cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or Em9 protein or fragments thereof in insect cell culture characterized by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 &mgr;g/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, &agr;-units an/or &bgr;-units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 &mgr;l.
Type:
Grant
Filed:
June 16, 2000
Date of Patent:
April 29, 2003
Assignees:
Stichting Instituut voor Dierhouderij en
Diergezondheid, Bayer AG
Inventors:
Dietmar Kretzdorn, Dirk Franciscus Marinus van de Wiel, Abraham Johannes de Smit, Robertus Jacobus Maria Moormann, Erik Kees Hamann
Abstract: A method for selecting eggs, in which the eggs are subjected to a Nuclear Magnetic Resonance (NMR) treatment for obtaining an NMR image and the eggs are selected on the basis of the NMR image.An apparatus for processing eggs includes egg supply means, egg selection means, and egg discharge means, the egg selection means having at least one apparatus for making at least one NMR image of an egg, and means being provided for controlling the egg discharge means on the basis of the at least one NMR image.
Type:
Grant
Filed:
July 6, 1998
Date of Patent:
November 21, 2000
Assignee:
Stichting Instituut Voor Dierhouderij en Diergezondheid