Abstract: The present invention relates to an expression system for the expression of proteins and peptides in a methanotrophic bacterium, preferably M. capsulatus. Further, the invention relates to the exportation and display of said peptides and proteins on the surface of said bacteria. The invention also describes a method for the production of a desired protein in M. capsulatus.

Abstract: The invention provides, in a method of perfusion magnetic resonance imaging of a region of interest in a vascularized animal subject, said method comprising administering a contrast agent bolus into the vascular system of said subject; determining the magnetic resonance signal strength si(t) for voxels (i) of said region of interest at a series of time values (t) over a period from before contrast agent arrival in said region of interest to at least the end of the first pass of said contrast agent through said region of interest; from the determined values si(t) for said signal strength and an arterial input function v(t), determining values for the tissue residue function ri(t) for each said voxel; and optionally generating an image of said region of interest from the determined values of ri(t); the improvement comprising generating from si(t) a voxel specific arterial function vi(t) and using said voxel specific arterial function to determine the values of the tissue residue function ri(t).

Abstract: The invention relates to a method for the investigation of intercellular communication and intercellular transport, wherein after singularisation cells are investigated for membrane tubes which contain F-actin and myosin, have a diameter of 50 to 400 nm, as a rule are up to 50 micrometers long or in same cases longer, and which span between the cells. The invention further relates to a method wherein the organelle transport between the cells is investigated.

Abstract: The invention provides, in a method of perfusion magnetic resonance imaging of a region of interest in a vascularized animal subject, said method comprising administering a contrast agent bolus into the vascular system of said subject; determining the magnetic resonance signal strength si(t) for voxels (i) of said region of interest at a series of time values (t) over a period from before contrast agent arrival in said region of interest to at least the end of the first pass of said contrast agent through said region of interest; from the determined values si(t) for said signal strength and an arterial input function v(t), determining values for the tissue residue function ri(t) for each said voxel; and optionally generating an image of said region of interest from the determined values of ri(t); the improvement comprising generating from si(t) a voxel specific arterial function vi(t) and using said voxel specific arterial function to determine the values of the tissue residue function ri(t).

Abstract: A method of disease prognosis which involves determining the genotype of a human or non-human mammal subject for at least one Fc receptor, and identifying whether the determined genotype corresponds to a benign or non-benign prognosis for a disease selected from multiple sclerosis, myasthenia gravis, diabetes mellitus, cerebrovascular diseases, artherosclerosis, and Addison's disease.