Abstract: The invention relates to a therapeutic composition, a method for producing a therapeutic composition, and the use of a cell-free substance, especially a cell-free bone or cartilage matrix. The disclosed therapeutic composition comprises at least a cell-free substance obtained from stimulated stem cells and/or precursor cells. Immunogenic reactions during in vivo therapeutic use are prevented by the fact that the therapeutic composition is free from cells and contains no typically antigenic cell components. The disclosed composition can therefore be universally used for the therapeutic purposes regardless of the origin of the stem cells and/or precursor cells and utilize the natural regenerative potency thereof in a highly efficient manner for replacing tissue, e.g. for a suitable bone and/or cartilage structure.

Abstract: Subject of the invention is a method for generating neural stem cells in vitro, wherein dental progenitor cells are isolated from soft tissue of tooth or wisdom tooth and cultivated until they form primary spheres which are then dissociated into single cells. These single cells are cultivated until they form spheroids and the spheroid-forming cells are separated to obtain neural stem cells.

Abstract: The invention relates to a method for isolation of multipotent stem cells from dental tissue in which the stem cells are extracted from a tissue structure and then cultured. The invention also relates to stem cells isolated by means of the method according to the invention as well as bone cells and nerve cells produced by means of the method according to the invention. The invention also relates to a method for producing a bank of stem cells in which the cells are stored by means of the method according to the invention. According to the present invention, the cells of a pad-like soft tissue that can be localized beneath the papilla directly on the apical side of an extracted immature tooth are cryopreserved in the tissue structure such that the tissue structure is disintegrated to extract the stem cells only after thawing.