Abstract: A system for managing resources and scheduling is disclosed. The system includes a client computer, software executable by the client computer for providing a user interface, a server in data communication with the client computer, a database for storing data in a hierarchical data structure, and a data fusion engine executing on the server. The hierarchical data structure includes a parent node and child nodes linked to the parent node. The data fusion engine is configured to log a workflow to at least one of the child nodes based on user input received via the user interface. A related method and software is also disclosed.

Abstract: A system for analyzing a biological sample may include at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source; a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured to receive the biological sample transferred from the sample acquisition device; a collection vessel for collecting the wrapped sample droplet from the droplet generator, the vessel configured to contain a carrier fluid for receiving and protecting the sample droplet; and an analysis system for analyzing the wrapped sample droplet and detecting products of a polymerase chain reaction.

Abstract: Alternative splicing events in SCN1A gene can lead to non-productive mRNA transcripts which in turn can lead to aberrant protein expression, and therapeutic agents which can target the alternative splicing events in SCN1A gene can modulate the expression level of functional proteins in Dravet Syndrome patients and/or inhibit aberrant protein expression. Such therapeutic agents can be used to treat a condition caused by SCN1A, SCN8A or SCN5A protein deficiency.

Abstract: A manufacturing method of an axial air moving device. A model of the axial air moving device includes a hub and blades. The axial projection of blades is partially overlapped in the axial direction of the hub. The model of the axial air moving device is parted in the axis direction of the hub. The blades are divided into multiple parting models non-overlapped in the axial projection. A mold manufacture using axial demolding and an injection molding are performed and the parting models are connected. Therefore, the axial air moving device with overlapped blades and better fluid performance is achieved through the axial demolding method.

Abstract: A system for managing resources and scheduling is disclosed. The system includes a client computer, software executable by the client computer for providing a user interface, a server in data communication with the client computer, a database for storing data in a hierarchical data structure, and a data fusion engine executing on the server. The hierarchical data structure includes a parent node and child nodes linked to the parent node. The data fusion engine is configured to log a workflow to at least one of the child nodes based on user input received via the user interface. A related method and software is also disclosed.

Abstract: Alternative splicing events in genes can lead to non-productive mRNA transcripts which in turn can lead to aberrant protein expression, and therapeutic agents which can target the alternative splicing events in genes can modulate the expression level of functional proteins in patients and/or inhibit aberrant protein expression. Such therapeutic agents can be used to treat a condition or disease caused by protein deficiency and/or mitochondrial function deficit.

Abstract: A system for processing a biological sample can include a droplet generation assembly comprising a plurality of first reservoirs configured to contain an aqueous sample and a plurality of second reservoirs configured to contain a carrier fluid immiscible with the aqueous sample. The plurality of first reservoirs and the plurality of second reservoirs can be arranged to be in respective flow communication in pairs of reservoirs comprising a first reservoir of the plurality of first reservoirs and a second reservoir of the plurality of second reservoirs constituting a plurality of pairs of reservoirs. The droplet generation assembly can further include a flow control system configured to control a pressure in the plurality of pairs of reservoirs so as to generate a flow of a series of volumes of the aqueous sample separated by the carrier fluid. The system can further include a thermocycling system.

Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.

Abstract: A system for processing a biological sample includes a substrate comprising a plurality of wells and a plurality of flow channels. The system further includes a flow control system comprising a manifold having a plurality of ports configured to fluidically couple to the plurality of wells, and one or more containment structures configured to contain carrier fluid and fluidically couple to the ports. The flow control system further includes a cradle configured to removably receive the substrate. The flow control system is configured to transmit pressure differential, via the manifold, to the plurality of wells so as to cause a plurality of sample volumes held by at least some wells of the plurality of wells to flow through respective flow channels and cause the carrier fluid to flow through the flow channels and form a plurality of droplets of the plurality of sample volumes separated by the carrier fluid.

Abstract: A method comprises flowing a plurality of sample droplets in a continuous flow of a carrier fluid, immiscible with the sample droplets, such that the droplets are separated from each other by the carrier fluid, wherein an average number of copies of target nucleic acid contained in each droplet the plurality of sample droplets is one or fewer. The method may further comprise subjecting the droplets to thermal cycling sufficient to allow amplification of the target nucleic acid, and detecting one or more of the presence or absence of amplified target nucleic acid in the droplets.

Abstract: This disclosure is related to a thin type counter-rotating axial air moving device. The ratio of the front hub diameter to the front blade diameter is about 0.3 to about 0.85. The front average pitch angle of the front blades is greater than about 46 degrees. The ratio of the rear hub diameter to the rear blade diameter is about 0.3 to about 0.85. The rear average pitch angle of the rear blades is less than about 38 degrees. The ratio of the total thickness to the greater one between the front blade diameter and the rear blade diameter is less than or equal to about 0.75.

Abstract: A counter-rotating axial air moving device includes a front rotor and a rear rotor. The front rotor includes a front hub and a plurality of front blades, and the number of the front blades is equal to or greater than 7 and equal to or less than 11. The rear rotor is disposed on the downstream side of the front rotor. The rear rotor includes a rear hub and a plurality of rear blades, and the number of the rear blades is equal to or greater than 6 and equal to or less than 10. The front rotor and the rear rotor are stacked with each other with a total thickness and a diameter. The ratio of the total thickness to the diameter is equal to or more than 0.91 and equal to or less than 1.5.

Abstract: A counter rotating axial air moving device structure is disclosed. The rear rotor includes a rear hub and rear blades, and a pitch angle of each of the rear blades increases gradually in a direction away from the rear hub. The front rotor, the rear rotor and the stator component are stacked with each other. The ratio of the thickness to the diameter is equal to or greater than about 0.25 and equal to or less than about 0.8. Therefore, a better performance curve is obtained, and the vibration and noise are avoided.

Abstract: This disclosure provides an air moving device with blade tip of variable curvature. The axial air moving device includes a hub and a plurality of blades. The blades are connected with the hub, and each blade is configured by stacking multiple wing sections continuously. Each blade includes a blade root and a blade tip. The span position of the blade at the blade root is defined as 0, and at the blade tip is defined as 1. The blade angle is defined by the nose-tail line of the wing section and the rotation direction of the axial air moving device. The blade angle of the wing section at the blade tip of the blade is at least 10 degrees less than the blade angle of the wing section at the span position of 0.8 of the blade.

Abstract: The air moving device includes a rotor and a stator. The quantity of the rotor blades is not less than 5 and not greater than 12. The average blade angle of rotor blades is not less than 45 degrees and is not greater than 64 degrees. The ratio of the hub diameter to the rotor diameter is not less than 0.4 and not greater than 0.79. The quantity of the stator blades is not less than 6 and not greater than 23. The average blade angle of stator blades is not less than 45 degrees and not greater than 70 degrees. The ratio of the total thickness of the air moving device to the rotor diameter is not less than 0.76 and not greater than 1.7. The ratio of the stator axial thickness to the rotor axial thickness is not less than 0.28 and not greater than 0.65.

Abstract: A biological sample analysis system including a sample preparation system forming droplets of segmented sample separated by a carrier fluid immiscible with the sample. The droplets include reaction mixtures for amplification of at least one target nucleic acid. A thermal cycling device having a sample block having a plurality of controlled thermal zones, and a containment structure in thermal communication with the plurality of controlled thermal zones. The containment structure receives and contains the droplets of segmented sample separated by the immiscible carrier fluid from the sample preparation system. A controller for controlling a temperature in each thermal zone of the sample block. A detection system detects electromagnetic radiation emitted from each of the droplets individually from the queue of droplets as they flow past the detection system. A positioning system to facilitate moving a queue of the droplets in the thermal cycling device relative to the detection system.

Abstract: A system for analyzing a biological sample may include at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source; a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured to receive the biological sample transferred from the sample acquisition device; a collection vessel for collecting the wrapped sample droplet from the droplet generator, the vessel configured to contain a carrier fluid for receiving and protecting the sample droplet; and an analysis system for analyzing the wrapped sample droplet and detecting products of a polymerase chain reaction.

Abstract: Provided herein are methods and compositions for increasing the expression of a protein, and for treating a subject in need thereof, e.g., a subject with deficient protein expression or a subject having a disease described herein.

Abstract: Provided herein are methods and compositions for treating a subject in need thereof, such as a subject with deficient SYNGAP1 protein or SCN1A protein expression or a subject having AD mental retardation 5 or Dravet syndrome.

Abstract: A biological sample analysis system including a sample preparation system forming droplets of segmented sample separated by a carrier fluid immiscible with the sample. The droplets include reaction mixtures for amplification of at least one target nucleic acid. A thermal cycling device having a sample block having a plurality of controlled thermal zones, and a containment structure in thermal communication with the plurality of controlled thermal zones. The containment structure receives and contains the droplets of segmented sample separated by the immiscible carrier fluid from the sample preparation system. A controller for controlling a temperature in each thermal zone of the sample block. A detection system detects electromagnetic radiation emitted from each of the droplets individually from the queue of droplets as they flow past the detection system. A positioning system to facilitate moving a queue of the droplets in the thermal cycling device relative to the detection system.