Patents Assigned to Stratagene California
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Patent number: 7118860Abstract: The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid in a sample, as well as a method of detecting or measuring a target nucleic acid in a sample. The invention comprises forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe having a secondary structure that changes upon binding of the probe to the target nucleic acid and further comprising a binding moiety. The invention also includes the steps of cleaving the cleavage structure with a nuclease to release a nucleic acid fragment to generate a signal, wherein generation of the signal is indicative of the presence of a target nucleic acid in a sample, and detecting and/or measuring the amount of the fragment captured by binding of a binding moiety to a capture element on a solid support.Type: GrantFiled: November 30, 2000Date of Patent: October 10, 2006Assignee: Stratagene CaliforniaInventors: Joseph A. Sorge, Anne M. Whalen
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Patent number: 7109178Abstract: The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.Type: GrantFiled: January 25, 2002Date of Patent: September 19, 2006Assignee: Stratagene CaliforniaInventors: Henry Ji, Alan Greener, Joseph A. Sorge, John Bauer, Richard Gibbs, Carsten-Peter Carstens
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Patent number: 7090978Abstract: The detection of insertions and/or deletions in reiterated nucleotide sequences in tissues provides an identification of neoplastic changes that are associated with malignancy. The mutations are preferably detected by PCR based amplification of target sequences using selected primers, followed by standard analytic procedures. The detection of these mutations is useful as a diagnostic tool for cancer development and has direct application for cancer prognosis.Type: GrantFiled: March 20, 2003Date of Patent: August 15, 2006Assignee: Stratagene CaliforniaInventors: Manuel Perucho, Miguel Angel Peinado, Yurij Ionov, Sergei Malkhosyan, Michael McClelland, John Welsh
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Patent number: 7083931Abstract: The present invention provides a polynucleotides encoding mutants of green fluorescent protein from Renilla reniformis, including humanized sequences which permit enhanced expression of the encoded polypeptides in mammalian cells.Type: GrantFiled: April 1, 2004Date of Patent: August 1, 2006Assignee: Stratagene CaliforniaInventor: Vanessa Elaine Gurtu
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Patent number: 7078220Abstract: The invention provided herein includes novel gram negative bacteria cells containing the Hte mutation. Other aspects of the invention include methods for rendering gram negative bacterial cells bearing the Hte region, such as E. coli cells competent for DNA transformation using any of a variety of competency inducing procedures. The competent cells of the subject invention may be frozen so as to provide for prolonged storage.Type: GrantFiled: March 12, 2004Date of Patent: July 18, 2006Assignee: Stratagene CaliforniaInventors: Alan Lewis Greener, Bruce Douglas Jerpseth
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Patent number: 7045328Abstract: Purified thermostable Pyrococcus furiosus DNA polymerase that migrates on a non-denaturing polyacrylamide gel faster than phosphorylase B and Taq polymerase and more slowly than bovine serum albumin and has an estimated molecular weight of 90,000–93,000 daltons when compared with a Taq polymerase standard assigned a molecular weight of 94,000 daltons.Type: GrantFiled: June 19, 2002Date of Patent: May 16, 2006Assignee: Stratagene CaliforniaInventor: Eric J. Mathur
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Patent number: 7005617Abstract: An apparatus for thermally cycling samples of a biological material including a thermal block assembly including a plurality of sample holders for receiving samples of biological material; a heat sink thermally coupled to the thermal block assembly, the heat sink transferring heat away from the thermal block assembly to ambient air in contact with the heat sink; a first heat source thermally coupled to the thermal block assembly to provide heat to the thermal block assembly; and a second heat source thermally coupled to the first heat source and configured to provide heat to a portion of the first heat source. The arrangement of the heat sink, first heat source and second heat source can provide substantial temperature uniformity among the plurality of sample holders. The invention also includes a method for thermally cycling samples of biological material.Type: GrantFiled: October 23, 2003Date of Patent: February 28, 2006Assignee: Stratagene CaliforniaInventor: Larry Richard Brown
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Patent number: 6977164Abstract: The invention relates to polynucleotides for HSV detection and the use of these polypeptides in kits and methods for HSV detection.Type: GrantFiled: November 9, 2004Date of Patent: December 20, 2005Assignee: Stratagene CaliforniaInventors: Cindy WalkerPeach, Dwight DuBois
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Patent number: 6962821Abstract: A method in which a temperature gradient is generated across a “gradient” block, and an apparatus comprising a block across which a temperature gradient can be generated. By setting up such a gradient, multiple reaction mixtures held in wells on the gradient block can be simultaneously run at temperatures which differ only slightly, thereby permitting an optimum temperature for the reaction to be quickly identified. In a preferred embodiment the gradient block is integrated into a thermal cycler used for nucleic acid amplification reactions.Type: GrantFiled: December 5, 2002Date of Patent: November 8, 2005Assignee: Stratagene CaliforniaInventors: John Lewis Danssaert, Robert James Shopes, Daniel Davis Shoemaker
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Patent number: 6958390Abstract: The invention relates to polynucleotides for HSV detection and the use of these polypeptides in kits and methods for HSV detection.Type: GrantFiled: June 14, 2000Date of Patent: October 25, 2005Assignee: Stratagene CaliforniaInventors: Cindy WalkerPeach, Dwight DuBois
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Patent number: 6946273Abstract: The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3? to 5? exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.Type: GrantFiled: October 27, 2000Date of Patent: September 20, 2005Assignee: Stratagene CaliforniaInventors: Joseph A. Sorge, Holly Hurlbut Hogrefe, Connie Jo Hansen
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Patent number: 6936428Abstract: The invention relates to proteins or polypeptides that comprise intramolecular dimers of fluorescent protein monomers. More specifically, the invention relates to recombinant polypeptides comprising a monomer of a fluorescent polypeptide, a linker peptide, and a second monomer of that fluorescent polypeptide, where the monomers form an intramolecular dimer. The invention also relates to nucleic acids encoding Intramolecular Dimer Fluorescent Proteins (IDFPs) and vectors comprising such nucleic acids. The invention further relates to methods of making IDFPs and methods of using them. IDFPs are, useful in any application suited for fluorescent proteins and are particularly useful in applications in which more than one fluorescent protein sharing complementary dimerization interfaces is present in the same mixture or is expressed in the same cell, because IDFPs do not form heterodimers.Type: GrantFiled: December 13, 2001Date of Patent: August 30, 2005Assignee: Stratagene CaliforniaInventors: Ronald W. Davis, Peter Vaillancourt
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Publication number: 20050106730Abstract: The invention encompasses compositions and methods which utilize a cell line comprising a stably integrated recombinant nucleic acid construct comprising a reporter gene operably lined to a recognition sequence for a sequence-specific DNA-binding protein and a stably integrated recombinant nucleic acid construct comprising a sequence encoding a fusion protein, the fusion protein comprising a sequence-specific DNA binding domain, wherein the DNA binding domain specifically binds the recognition sequence, and a conditionally active transactivation domain, wherein activation of the conditionally active transactivation domain is dependent on protein phosphorylation and/or protein: protein interaction, and wherein binding of the fusion protein to the recognition sequence results in transactivation of the reporter gene expression when the transactivation domain fused to the DNA binding domain is activated.Type: ApplicationFiled: April 5, 2004Publication date: May 19, 2005Applicant: Stratagene CaliforniaInventor: Chao-Feng Zheng
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Patent number: 6893819Abstract: The invention relates to a method of detecting a target nucleic acid by linear amplification. The invention also relates to a method of detecting a target nucleic acid by amplification. The method of the invention includes the steps of forming at least a first cleavage structure comprising at least one flap, cleaving the cleavage structure with a cleavage means to release the at least one flap and detecting the at least one flap. In one embodiment of the invention, the method includes the additional steps of forming at least a second cleavage structure comprising the at least one released flap from the first cleavage structure cleaving the second cleavage structure with a cleavage means to release at least one flap from the second cleavage structure and detecting released flaps from the first or second cleavage structure.Type: GrantFiled: November 21, 2000Date of Patent: May 17, 2005Assignee: Stratagene CaliforniaInventor: Joseph A. Sorge
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Patent number: 6890750Abstract: The invention encompasses compositions and methods which utilize a cell line comprising a stably integrated recombinant nucleic acid construct comprising: a reporter gene operably linked to a recognition sequence for a sequence-specific DNA-binding protein; and a stably integrated recombinant nucleic acid construct comprising a sequence encoding a fusion protein, the fusion protein comprising a sequence-specific DNA binding domain, wherein the DNA binding domain specifically binds the recognition sequence, and a conditionally active transactivation domain of CHOP, wherein binding of the fusion protein to the recognition sequence results in transactivation of the reporter gene when the transactivation domain fused to the DNA binding domain is activated.Type: GrantFiled: August 11, 2000Date of Patent: May 10, 2005Assignee: Stratagene CaliforniaInventor: Chao-Feng Zheng
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Patent number: 6878905Abstract: A flexible heating cover assembly for an apparatus for heating samples of biological material with substantial temperature uniformity includes a housing having a plurality of engageable enclosure components; a resistive heater having a plurality of heater element areas; a heater backing plate providing stability to the resistive heater; a force distribution system that distributes a force over the heater backing plate; and a support plate providing stiffness for the force distribution system, wherein the arrangement of the resistive heater, the heater backing plate, the force distribution system and the support plate provide substantial temperature uniformity among a plurality of sample tubes for receiving samples of biological material. The flexible heating cover assembly improves the uniformity, efficiency, quality, reliability and controllability of the thermal response during thermal cycling of the biological material.Type: GrantFiled: March 29, 2004Date of Patent: April 12, 2005Assignee: Stratagene CaliforniaInventors: Larry R. Brown, William D. Brumley, Kenneth J. Zajac
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Patent number: 6869797Abstract: This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.Type: GrantFiled: June 30, 2003Date of Patent: March 22, 2005Assignee: Stratagene CaliforniaInventors: Alan L. Greener, Bruce D. Jerpseth
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Patent number: 6858393Abstract: The invention relates to acyclic chain terminator nucleotide analogs. More particularly, the invention relates to phosphonomethoxyethyl nucleotide analogs and detectably labeled versions thereof, especially fluorescently labeled versions thereof. The invention further relates to the use of chain terminating phosphonomethoxyethyl nucleotide analogs in methods of synthesizing a polynucleotide, labeling a polynucleotide, determining polynucleotide sequence information, and kits therefor.Type: GrantFiled: March 13, 2002Date of Patent: February 22, 2005Assignee: Stratagene CaliforniaInventors: Jack Dewayne Anderson, Jeffrey Carl Braman
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Patent number: 6841357Abstract: A method of titering adeno-associated virus particles in a sample, said method comprising the steps of contacting target cells with a DNA synthesis inhibitor and an agent that increases the activity of the CMV immediate early promoter; contacting target cells treated as in step (a) with a sample containing adeno-associated virus particles; and determining the number of target cells infected by said adeno-associated virus particles in said sample, wherein said number of target cells infected is directly proportional to the titer of said particles in said sample, thereby determining the titer if said adeno-associated virus particles.Type: GrantFiled: March 29, 2002Date of Patent: January 11, 2005Assignee: Stratagene CaliforniaInventors: Peter Edward Vaillancourt, Vivian Qingqing Zhang
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Patent number: 6825330Abstract: The invention relates to novel platinum-based compounds for labeling biomolecules. Platinum based labeling compounds according to the invention irreversibly attach to a target biomolecule via coordination of a platinum (II) metal center with N or S atoms on the target biomolecule. The invention relates to the novel compounds themselves, methods of making the platinum-based labeling compounds, probes labeled with such compounds, methods of making such labeled probes, and kits comprising the novel platinum-based labeling compounds and/or probes labeled with them. The invention also relates to methods of using probes labeled with platinum-based labeling compounds of the invention, particularly array and microarray hybridization methods.Type: GrantFiled: March 1, 2002Date of Patent: November 30, 2004Assignee: Stratagene CaliforniaInventors: Jeffrey Braman, Haoqiang Huang