Abstract: A method in which a temperature gradient is generated across a “gradient” block, and an apparatus comprising a block across which a temperature gradient can be generated. By setting up such a gradient, multiple reaction mixtures held in wells on the gradient block can be simultaneously run at temperatures which differ only slightly, thereby permitting an optimum temperature for the reaction to be quickly identified. In a preferred embodiment the gradient block is integrated into a thermal cycler used for nucleic acid amplification reactions.
Type:
Application
Filed:
December 5, 2002
Publication date:
August 21, 2003
Applicant:
Strategene
Inventors:
John Lewis Danssaert, Robert James Shopes, Daniel Davis Shoemaker
Abstract: An apparatus for chromatography of DNA, RNA, proteins and other molecules includes the use of a column adapted to hold a chromatography material and a sample to be filtered. A pneumatic pressure differential is applied across the column and the sample is urged through the chromatography material. A selected portion of the sample may then be collected.
Type:
Grant
Filed:
January 30, 1992
Date of Patent:
January 3, 1995
Assignee:
Strategene
Inventors:
William D. Huse, Anthony M. Sorge, Keith V. Sylvester