Abstract: An improved reagent system for preparation of cells for flow cytometry having a physiologically compatible salt solution composition including a lysing agent, an agent for minimizing white blood cell lysis, and optionally a preservative.

Abstract: A method of emulating with a cyanide-free lyse solution, the measurement of hemoglobin in whole blood using a cyanide-containing lyse solution. Such an illustrative method includes: a) combining a predetermined amount of the whole blood sample with a predetermined amount of a cyanide-free lyse solution to form a mixture, b) developing the mixture to a molar absorbtivity (?) in the range of about 12.4 to 12.6 mM?1cm?1, and c) measuring a level of light absorbance of the mixture at a wavelength of about 540 nm. In one preferred illustrative embodiment, the cyanide-free lyse solution includes: a quaternary ammonium salt surfactant, an anionic surfactant, a hemoglobin binding agent selected from the group consisting of imidazole or hydroxylamine, and an aqueous medium. In one preferred embodiment, the molar absorbtivity is developed to a value of about 12.5 mM?1cm?1. That is to say to a level that is substantially identical to that of the prior art standard, cyanomethemoglobin.

Abstract: An improved reagent system for preparation of cells for flow cytometry having a physiologically compatible salt solution composition including a lysing agent, an agent for minimizing white blood cell lysis, and optionally a preservative.

Abstract: A reagent system for substantially lysing red blood cells in a whole blood sample prior to leukocyte analysis, the reagent system includes: a first reagent for substantially lysing the red blood cells in the whole blood sample, and a second reagent for quenching the activity of the first reagent, wherein the second reagent includes a base and has a pH value of about 8 to 12. A final acidic media, ranging from about pH 4 to about 6, is used to stabilize the white blood cells and continuously remove red blood cell fragments. The first reagent is formulated to include: a saponin compound; an acid selected from the group consisting of halogenated carboxylic acids, phosphoric acid or combinations thereof and optionally a surfactant.

Abstract: The claimed subject matter comprises a device to collect and preserve cells comprising of: (1) a collection container comprised of a tube having an open end and a closed end, a closure in the open end of the tube, a vacuum drawn to a predetermined level inside the container; and (2) compounds including an anticoagulant agent and a fixative agent, wherein the compounds are in a sufficient amount to preserve said cells' original morphology and antigenic sites without significant dilution of said cells, and thereby allowing said cells to be directly analyzed by a flow cytometer without further treatment.

Abstract: An improved control for a hematology analyzer. In one embodiment, blood cells are treated for permitting the cells to simulate nucleated red blood cells for detection or analysis by the hematology analyzer.

Abstract: A method of emulating with a cyanide-free lyse solution, the measurement of hemoglobin in whole blood using a cyanide-containing lyse solution. Such an illustrative method includes: a) combining a predetermined amount of the whole blood sample with a predetermined amount of a cyanide-free lyse solution to form a mixture, b) developing the mixture to a molar absorbtivity (&egr;) in the range of about 12.4 to 12.6 mM−1cm−1, and c) measuring a level of light absorbance of the mixture at a wavelength of about 540 nm. In one preferred illustrative embodiment, the cyanide-free lyse solution includes: a quaternary ammonium salt surfactant, an anionic surfactant, a hemoglobin binding agent selected from the group consisting of imidazole or hydroxylamine, and an aqueous medium. In one preferred embodiment, the molar absorbtivity is developed to a value of about 12.5 mM−1cm−1. That is to say to a level that is substantially identical to that of the prior art standard, cyanomethemoglobin.

Abstract: An improved control for a hematology analyzer. In one embodiment, blood cells are treated for permitting the cells to simulate nucleated red blood cells for detection or analysis by the hematology analyzer.

Abstract: An improved control for a hematology analyzer. In one embodiment, blood cells are treated for permitting the cells to simulate nucleated red blood cells for detection or analysis by the hematology analyzer.

Abstract: An improved control for a hematology analyzer. In one embodiment, blood cells are treated for permitting the cells to simulate nucleated red blood cells for detection or analysis by the hematology analyzer.

Abstract: A control and system for blood testing, including a control having stabilized blood cells that have been introduced into a suspension including an aggregating agent and preferably a surfactant. The control and system is used advantageously for indirect acute protein plasma measurement, including erythrocyte sedimentation rate testing.

Abstract: An improved reagent system for preparation of cells for flow cytometry having a physiologically compatible salt solution composition including a lysing agent, an agent for minimizing white blood cell lysis, and optionally a preservative.

Abstract: A system, composition and method for the stabilization of biological specimens, which employs a chemical fixative.

Abstract: Hematology control compositions and systems used to measure a plurality of parameters in a blood sample are provided. The hematology control compositions are particularly useful as a control for multi-parameter, automated instrument systems. The control compositions comprise a reticulocyte component, a white blood cell component, a red blood cell component, a nucleated red blood cell component, a platelet component and a reticulated platelet component. Methods of making and using the control compositions are also provided.

Abstract: Hematology control compositions and systems used to measure a plurality of parameters in a blood sample are provided. The hematology control compositions are particularly useful as a control for multi-parameter, automated instrument systems. The control compositions comprise a reticulocyte component, a white blood cell component, a red blood cell component, a nucleated red blood cell component, a platelet component and a reticulated platelet component. Methods of making and using the control compositions are also provided.

Abstract: Hematology control compositions and systems used to measure a plurality of parameters in a blood sample are provided. The hematology control compositions are particularly useful as a control for multi-parameter, automated instrument systems. The control compositions comprise a reticulocyte component, a white blood cell component, a red blood cell component, a nucleated red blood cell component, a platelet component and a reticulated platelet component. Methods of making and using the control compositions are also provided.

Abstract: Hematology control compositions and systems used to measure a plurality of parameters in a blood sample are provided. The hematology control compositions are particularly useful as a control for multi-parameter, automated instrument systems. The control compositions comprise a reticulocyte component, a white blood cell component, a red blood cell component, a nucleated red blood cell component, a platelet component and a reticulated platelet component. Methods of making and using the control compositions are also provided.

Abstract: A three-phase suspension suitable for use as an erythrocyte sedimentation rate (ESR) control having the following three components: (1) a synthetic plasma base, (2) an aggregating agent such as a high molecular weight polymer or combination of high molecular weight polymers, and (3) chemically fixed mammalian red blood cells. The control is designed to allow the user to monitor the accuracy and precision of analytical methods for determining the sedimentation rate of human erythrocytes in whole blood specimens. Chemical fixing of the red blood cells provides the ESR control with the capability of providing useful results in the presence of citrate and/or saline.

Abstract: A system, composition and method for the stabilization of biological specimens, which employs a chemical fixative.

Abstract: A control and system for blood testing, including a control having morphologically fixed and stabilized blood cells that have been added to a diluent. The control and system can be used advantageously for indirect acute protein plasma measurement, including erythrocyte sedimentation rate testing.