Abstract: The present invention relates to a method for manufacturing a triple cross-linked collagen, which comprises the following steps: providing a soluble collagen sample; mixing the collagen sample with a first cross-linking agent to form a one cross-linked collagen; mixing the first cross-linked collagen with a second cross-linking agent to form a second cross-linked collagen; and mixing the second cross-linked collagen with a third cross-linking agent to form a triple cross-linked collagen, wherein each of the first cross-linking agent, the second cross-linking agent, and the third cross-linking agent is selected from the group consisting of an aldehyde cross-linking agent, an imine cross-linking agent, and an epoxide cross-linking agent. In addition, the first cross-linking agent is different form the second cross-linking agent, and the third cross-linking agent is different form the first cross-linking agent and the second cross-linking agent.

Abstract: The present invention relates to a method for manufacturing a triple cross-linked collagen, which comprises the following steps: providing a soluble collagen sample; mixing the collagen sample with a first cross-linking agent to form a one cross-linked collagen; mixing the first cross-linked collagen with a second cross-linking agent to form a second cross-linked collagen; and mixing the second cross-linked collagen with a third cross-linking agent to form a triple cross-linked collagen, wherein each of the first cross-linking agent, the second cross-linking agent, and the third cross-linking agent is selected from the group consisting of an aldehyde cross-linking agent, an imine cross-linking agent, and an epoxide cross-linking agent. In addition, the first cross-linking agent is different form the second cross-linking agent, and the third cross-linking agent is different form the first cross-linking agent and the second cross-linking agent.

Abstract: A biodegradable filler for restoration of alveolar bones is disclosed, which includes: first cross-linked collagen fibers prepared from reacting Non-crosslinked collagen fibers with a cross-linking agent; and supporting particles which are biomedical ceramic particles, bioactive glass, or a combination thereof, and distributed among the first cross-linked collagen fibers.

Abstract: In a long-lasting collagen and its manufacturing method, a pig skin is gone through processes of scraping extra tissues, removing fats, imbibition, digesting, centrifugal separation, salting-out, collecting lower-layer precipitate and freeze-drying to form a collagen, and the collagen is mixed with ?-PGA, and then a glutaraldehyde solution is added and mixed uniformly to perform a first crosslinking and form the long-lasting collagen, so as to overcome the shortcomings of a conventional collagen having a short storage time, a requirement of applying the collagen repeatedly, and a high concentration of remained glutaradldehyde which is biologically poisonous to human bodies.

Abstract: The present invention relates to a method for manufacturing a collagen membrane, which comprises the following steps: preparing a collagen slurry; degassing the collagen slurry, and then forming collagen gel at a predetermined collagen concentration, ionic strength, pH value, and temperature; removing water in the collagen gel by an absorbent device to form a collagen mat; and flattening and drying the collagen mat under vacuum by a gel dryer. The present invention provides the collagen membranes manufactured by the above-mentioned method. Such membranes are of good biocompatibility, high tensile strength, hydrophilicity, flexibility and easy handling features. Hence, these membranes can function as medical material suitable for repair of tissue and wound healing.

Abstract: In the present invention, a biodegradable bone graft is disclosed, which includes: a scaffold made of a biodegradable material; and a collagen-embedding matrix portion which completely encompasses the scaffold. The above-mentioned bone graft can increase the micro-porosity of the scaffold to enable cells to grow adhesively thereon. Compared with the scaffold only, the above-mentioned bone graft has high hydrophilicity. Hence, the bone graft of the present invention can efficiently retain tissue fluid, cell growth factors, blood and/or bone marrow which are mixed with the bone graft beforehand to achieve osteoinduction. Furthermore, the collagen-embedding matrix portion can also serve as a carrier to encompass other bone graft materials and drug molecules. The present invention also relates to a method for manufacturing the above-mentioned bone graft.