Abstract: Provided is a method for developing biological trace evidence on a porous object, including immersing a porous object in a biological fluorescent development reagent or spraying the reagent on the porous object, drying the porous object in an environment having a relative humidity of less than 40% at a temperature of 50° C.-120° C. irradiating the dried porous object with a laser having a wavelength of 532 nm and a full width at half-maximum of less than 1 nm, controlling a surface of the porous object with an illuminance of over 300,000 lux, and using a cut-off filter under 540 nm to develop the biological trace evidence. A raw material formulation of the reagent is, in percent by weight, 0.02%-0.5% of indanedione, 4%-10% of ethyl acetate, 0.5%-1.5% of glycerol, 5%-15.5% of pure alcohol, and 73.5%-90% of petroleum ether.

Abstract: A method for developing and extracting biological trace evidence comprises the following steps: (1) using a biological fluorescent development reagent to process a porous carrier so as to develop biological trace evidence on the porous carrier, wherein a raw material formulation of the biological fluorescent development reagent is, in percent by weight: 0.01%-0.5% of indanedione, 4%-10% of ethyl acetate, 0.5%-1.5% of glycerol, 5%-15.5% of pure alcohol, and 73.5%-90% of petroleum ether; and (2) extracting the biological trace evidence to obtain DNA information of the biological trace evidence. For crime investigators who need to extract DNA evidence, the method enables targeted extraction of physical evidence, thereby greatly reducing workload, and furthermore, the method can also be used to develop and extract obscure or trace evidence, such as a fingerprint on a garment, thereby greatly improving investigation efficiency.