Abstract: The present invention provides a method for producing lactic acid in a recombinant yeast cell culture using glucose as carbon source comprising a first, seed fermentation stage to produce biomass wherein the yeast is cultivated in a culture medium at a pH of 5 to 7, followed by a second, a production fermentation stage with biomass from the seed fermentation to produce lactic acid, wherein the yeast is cultivated in a culture medium at low p H using a yeast strain that is engineered to have lactate dehydrogenase (LDH) activity and optionally has decreased or knocked-out pyruvate decarboxylase (PDC) activity.
Type:
Grant
Filed:
April 14, 2017
Date of Patent:
March 16, 2021
Assignee:
SYCONIUM LACTIC ACID GMBH
Inventors:
Magnus Ask, Rakesh Koppram, Diethard Mattanovich, Michael Sauer