Abstract: A hyperspectral imaging apparatus and methods for performing hyperspectral imaging of a surgical field, involving: an external optical imaging device for externally imaging internal tissue through a surgical access port, the access port having a port wall having a light-diffusing surface texture, whereby reflectance of the port wall is decreasable, and the external optical imaging device having an illuminated exoscope, the illuminated exoscope having: a longitudinal housing; an optical imaging assembly provided within the longitudinal housing; an imaging camera interfaced with the optical imaging assembly for detecting images collected by the optical imaging assembly; and one or more illumination sources supported by the longitudinal housing, wherein an illumination axis associated with each illumination source is offset from an imaging axis of the optical imaging assembly; a remote light source; a spectral filtering device in optical communication with the remote light source; and a light guide having a prox