Abstract: Provided are an anti-PD-L1 antibody, and a preparation method therefor and an application thereof. The antibody is obtained by a computer-aided design, and the anti-PD-L1 antibody comprises an antibody I and/or an antibody II; an antibody sequence is subjected to simulation analysis and design by bioinformatics modeling without complicated and cumbersome immune reactions; the antibody is designed and transferred into a host cell by molecular biological construction, and expressed in the host cell and purified to obtain the antibody with a PD-L1 antigen binding activity.