Abstract: The present invention relates to a high-density western blot array analysis method capable of simultaneously checking whether specific proteins are present in a plurality of samples, or simultaneously checking whether a plurality of proteins are present in a single sample, and according to the analysis method of the present invention, there are advantages of: reducing sample volume; enabling analysis even with a very small amount of antibodies; enabling the time required for testing to be remarkably shortened and a device to be compactly formed without high-priced equipment; and enabling analysis to proceed without restrictions on test formats.