Abstract: The present disclosure relates to a local heating type 3D printer head that may minimize material deformation and prevent or delay denaturation by locally heating the area around a nozzle through which a printing composition is ejected, characterized in that the local heating type 3D printer head includes: a head frame in the form of a plate; a syringe provided at the front of the head frame to receive the printing composition and to eject the printing composition through a lower nozzle part; and a heating part provided on an outer peripheral surface of the lower part of the syringe to locally heat the area around the nozzle part.

Abstract: The present disclosure relates to a 3D printing method for constant line width, in which the ejected material has a constant line width and 3D structures may be printed clearly even when only one layer is stacked, characterized in that the method includes: ejecting a synthetic polymeric material accommodated in a syringe on a printing bed by extrusion through a nozzle and stacking it; raising the nozzle to a certain height from an ejected lower layer material to produce a thin ejected layer; and printing a material ejected through the nozzle in a manner of layering it on top of a lower layer to produce a 3D structure.

Abstract: Disclosed is a slit nozzle for large area printing in which printing material is ejected in a line way, in which batch printing of a large area is enabled, so that printing time is shortened and uniformity is improved, characterized in that a slit nozzle for large area printing comprises: a syringe in which printing material for printing is received; a pair of manifolds connected to the syringe and formed with a chamber for storing and ejecting printing material ejected from the syringe in the form of a line; and a seamplate positioned between the pair of manifolds to ensure that printing material ejected from the syringe uniformly fills the chamber and determines the ejection thickness of the ejected printing material.

Abstract: The present disclosure relates to a blowing device for improving three-dimensional (3D) structure printing processing, which may improve printing processability by rapidly solidifying printing material ejected from a nozzle, and is characterized in that it includes a blower jig installed at the lower part of a syringe in which printing material is received and ejected, and having a spraying hole which sprays fluid to reduce the temperature of the printing material ejected from the nozzle of the syringe and around the nozzle to shorten the solidification time of the printing material; and a pneumatic hose connected to the blower jig to deliver the fluid at a normal pressure.

Abstract: The present invention relates to a multi-stage decellularized mammal-derived tissue matrix, prepared by a method comprising: a preparation step for preparing tissue of a non-human mammal; a pretreatment step for pretreating the tissue; a first inactivation step for inactivating a virus included in the pretreated tissue using alcohol; a primary decellularization step for removing cells from the virus-inactivated tissue using an aqueous basic solution; a secondary decellularization step for removing cells by enzymatically treating the primary decellularized tissue; and a second inactivation step for inactivating a virus included in the decellularized tissue using an acid, and wherein the DNA content in the tissue subjected to the second inactivation step is 50 ng/mg or less, and the reduction rate (L) of the elastin content in the tissue subjected to the pretreatment step to the second inactivation step is 20% or less; and a method for preparing the same.

Abstract: The present invention relates to a multi-stage decellularized mammal-derived tissue supplement, prepared by a method comprising: a preparation step for preparing tissue of a non-human mammal; a pretreatment step for pretreating the tissue; a first inactivation step for inactivating a virus included in the pretreated tissue using alcohol; a primary decellularization step for removing cells from the virus-inactivated tissue using an aqueous basic solution; a secondary decellularization step for removing cells by enzymatically treating the primary decellularized tissue; and a second inactivation step for inactivating a virus included in the decellularized tissue using an acid, and wherein the DNA content in the tissue subjected to the pretreatment step to the second inactivation step is 50 ng/mg or less, and the degradation time using 100 U/ml of collagenase is at least 90 hours; and a method for preparing the same.

Abstract: The present invention relates to a method for producing an asymmetric hair follicle spheroid comprising: an accommodating part preparation step of preparing a bio-ink accommodating part in which a partitioning member providing a plurality of partitioned spaces is accommodated; a bio-ink supplying step of positioning the partitioning member in the bio-ink accommodating part and supplying bio-ink containing hydrogel to each partitioned space of the partitioning member; and a discharge step of applying pressure to the bio-ink accommodating part and discharging the bio-ink accommodated in the partitioned spaces through a single nozzle. The asymmetric hair follicle spheroid produced according to the present disclosure allows the direction of hair growth to be controlled.

Abstract: The present invention relates to a three-dimensional (3D) printing system for manufacturing an artificial blood vessel and a method for manufacturing an artificial blood vessel using the same, wherein a cylindrical support having a hollow 3D porous structure including a thermoplastic polymer is manufactured and vertically fixed, and a hydrogel divided into at least two sections is discharged into the support, thereby maintaining the structure and shape constantly even after printing and manufacturing an artificial blood vessel having a hollow multilayered structure.

Abstract: Proposed is a non-fixing implant made of a raw material including a biomaterial and a ceramic-based composite material having excellent osteoconductivity in addition to a polymer. The non-fixing implant can be accurately secured to a gap between the skull and the bone flap and can be conveniently used. Further proposed is a method of manufacturing the non-fixing implant. The non-fixing implant includes a flexible wedge deformable to conform to the external contour of the bone flap and a plurality of wings connected to an upper or lower portion of the flexible wedge and extending to both sides of the flexible wedge. The wings have a porous structure. The wings on one side will be positioned on the bone flap and the wings on the other side will positioned on the skull. The non-fixing implant has the advantage of being capable of accurately filling a defect formed by craniotomy, has improved biocompatibility and bone bonding ability, and allows tissue invasion.

Abstract: Proposed is a method for preparing an animal tissue-derived biomaterial, an animal tissue-derived biomaterial prepared thereby, and a 3D printing method using the same, the method including: a decellularization step of removing cells from the tissue; a liquefaction step of liquefying the extracellular matrix of the decellularized tissue using an acid protease; a filtration step of filtering the decellularized extracellular matrix solution obtained through the liquefaction step; and a sterilization step of sterilizing the mixture obtained through the filtration step.

Abstract: The present invention is a printing device using multiple inks and a printing method using thereof, and more specifically, relates to a three-dimensional printing method of a printed product with a cross-sectional pattern comprising a step of providing different inks into each partitioned spaces and applying the same pressure condition to the inks retained in the ink-receiving part, thereby extruding the inks into a single extruding port to prepare and print an extruded ink product, using the printing device comprising an ink extruding member comprising an ink-receiving part receiving the multiple inks in each partitioned space, and an ink-extruding part equipped with a single passage in which the multiple inks received in the ink-receiving part are passed together.

Abstract: Proposed is a bioink supply system and, more particularly, proposed is a bioink supply system including: a hydrogel storage part; cell storage part; a mixing part configured to receive and mix a hydrogel and a cell solution from the hydrogel storage part and the cell storage part; a sensor part configured to measure a level of bioink inside a syringe; and a controller configured to receive a signal from the sensor part and maintain a constant level of the bioink inside the syringe, in which the mixing part supplies, to the syringe, the bioink prepared by mixing the hydrogel and the cell solution. The bioink supply system can continuously supply an active bioink to a syringe of a bioprinter during 3D bioprinting, and thus can continuously print large-scale biotissue, a plurality of organoids, organ-on-a-chip devices, etc.

Abstract: The present invention relates to a method of filling different two-kinds of multiple inks into an ink extruding member for a three-dimensional print and a method of three-dimensional printing using the filled ink, and relates to a three-dimensional printing method using multiple inks comprising a step of applying pressure to the retained multiple inks and extruding it into a single extruding port of the extruding part to prepare an ink extruded product and printing the ink extruded product.

Abstract: A cell printing apparatus according to the present disclosure comprises: a nozzle through which a liquid cell substance is discharged; a container in which the liquid cell substance discharged through the nozzle is laminated into a three-dimensional structure; and a supply unit for supplying the liquid heated to a predetermined temperature to the container.

Abstract: A cell printing apparatus of the present disclosure comprises a nozzle configured to discharge a heat-sensitive cell printing composition; and a heating unit for transferring heat to the upper side of the heat-sensitive cell printing composition which is discharged from the nozzle to be laminated. A predetermined space is formed between the heating unit and the nozzle so that the heating unit does not contact with the nozzle.