Abstract: A method for selectively orienting molecules on a surface of a solid support. The method includes: (a) attaching a linker molecule to the surface of the solid support, the linker molecule including a head group that is capable of binding to the solid support, and a tail group that is capable of chelating to a metal ion; (b) subsequently treating the solid support with a solution containing the metal ion; (c) attaching a metal ion chelating tag to the molecules to form tagged molecules; and (d) capturing the tagged molecules on the solid support by contacting it with the tagged molecules to form a monolayer of molecules on the surface of the solid support in which a majority of the molecules are held in the same orientation with respect to the surface.
Type:
Application
Filed:
June 10, 2010
Publication date:
September 30, 2010
Applicant:
TACNIA PTY LTD
Inventors:
Lisandra Lorraine Martin, Daniel Luke Johnson
Abstract: A method for selectively orienting molecules on a surface of a solid support. The method includes the steps of: (a) attaching a linker molecule to the surface of the solid support, the linker molecule including a head group that is capable of binding to the solid support, and a tail group that is capable of chelating to a metal ion; (b) subsequently treating the solid support with a solution containing the metal ion; (c) attaching a metal ion chelating tag to the molecules to form tagged molecules; and (d) capturing the tagged molecules on the solid support by contacting it with the tagged molecules to form a monolayer of molecules on the surface of the solid support in which a majority of the molecules are held in the same orientation with respect to the surface. The invention also provides a sensor chip formed using the methods of the invention.
Type:
Grant
Filed:
January 6, 2006
Date of Patent:
July 20, 2010
Assignee:
Tacnia Pty Ltd
Inventors:
Lisandra Lorraine Martin, Daniel Luke Johnson