Patents Assigned to TAGCYX BIOTECHNOLOGIES
  • Patent number: 10695362
    Abstract: This invention is intended to enhance and improve the resistance of a single- or double-stranded nucleic acid fragment comprising a base sequence of a functional nucleic acid to degradation by nucleolytic enzymes in a simple and cost-effective manner. The single- or double-stranded nucleic acid fragment comprises, ligated to at least one end thereof, hairpin-shaped DNA comprising: (A) a nucleic acid region comprising 2 to 5 arbitrary nucleotides; (B) a nucleic acid region comprising a “gna” or “gnna” base sequence, wherein each “n” represents “g”, “t”, “a”, or “c”, a base analogue, or a modified base; and (C) a nucleic acid region comprising a base sequence complementary to the nucleic acid region (A), sequentially from the 5? end toward the 3? end.
    Type: Grant
    Filed: April 23, 2018
    Date of Patent: June 30, 2020
    Assignee: TAGCYX BIOTECHNOLOGIES INC.
    Inventors: Ichiro Hirao, Michiko Hirao, Shuang Liu, Iwao Nozawa
  • Publication number: 20190345499
    Abstract: An object of the present invention is to provide a convenient and low-cost method for enhancing the stability of a DNA aptamer and/or its capacity to bind to a target molecule, and a DNA aptamer obtained by the method. The object is solved by substituting an internal hairpin structure (stem-loop structure) of the DNA aptamer with a structure called mini-hairpin structure and optionally increasing GC pairs in a stem portion of the DNA aptamer.
    Type: Application
    Filed: July 24, 2019
    Publication date: November 14, 2019
    Applicant: TAGCYX BIOTECHNOLOGIES
    Inventors: Ichiro Hirao, Michiko Hirao, Kenichiro Matsunaga
  • Publication number: 20180348220
    Abstract: The purpose of the present invention is to provide an aptamer to a cancer cell, said aptamer being superior to conventional aptamers in binding ability, specificity, and/or stability. To solve this problem, provided is a DNA aptamer binding to a cancer cell, said DNA aptamer containing an artificial base(s).
    Type: Application
    Filed: October 25, 2016
    Publication date: December 6, 2018
    Applicant: TAGCYX BIOTECHNOLOGIES
    Inventors: Kazunobu FUTAMI, Ichiro HIRAO, Michiko HIRAO
  • Publication number: 20180346912
    Abstract: An object of the present invention is to provide a convenient and low-cost method for enhancing the stability of a DNA aptamer and/or its capacity to bind to a target molecule, and DNA aptamer obtained by the method. The object is solved by substituting an internal hairpin structure (stem-loop structure) of the DNA aptamer with a structure called mini-hairpin structure and optionally increasing GC pairs in a stem portion of the DNA aptamer.
    Type: Application
    Filed: March 4, 2016
    Publication date: December 6, 2018
    Applicant: TAGCYX BIOTECHNOLOGIES
    Inventors: Ichiro HIRAO, Michiko HIRAO, Kenichiro MATSUNAGA
  • Publication number: 20180305695
    Abstract: The purpose of the present invention is to provide an aptamer for vWF, which is superior in a binding ability, a dissociation rate, and/or stability to the conventional nucleic acid aptamers. The present invention can solve the problem by a DNA aptamer which contains artificial nucleotides and binds to vWF.
    Type: Application
    Filed: October 25, 2016
    Publication date: October 25, 2018
    Applicant: TAGCYX BIOTECHNOLOGIES
    Inventors: Ichiro Hirao, Michiko HIRAO, Kenichiro MATSUNAGA
  • Publication number: 20180243329
    Abstract: This invention is intended to enhance and improve the resistance of a single- or double-stranded nucleic acid fragment comprising a base sequence of a functional nucleic acid to degradation by nucleolytic enzymes in a simple and cost-effective manner. The single- or double-stranded nucleic acid fragment comprises, ligated to at least one end thereof, hairpin-shaped DNA comprising: (A) a nucleic acid region comprising 2 to 5 arbitrary nucleotides; (B) a nucleic acid region comprising a “gna” or “gnna” base sequence, wherein each “n” represents “g”, “t”, “a”, or “c”, a base analogue, or a modified base; and (C) a nucleic acid region comprising a base sequence complementary to the nucleic acid region (A), sequentially from the 5? end toward the 3? end.
    Type: Application
    Filed: April 23, 2018
    Publication date: August 30, 2018
    Applicant: TAGCYX BIOTECHNOLOGIES
    Inventors: Ichiro HIRAO, Michiko HIRAO, Shuang LIU, Iwao NOZAWA
  • Patent number: 9857362
    Abstract: An object of the present invention is to develop and provide a method for efficiently and conveniently producing a nucleic acid aptamer, particularly, a DNA aptamer, having high specificity for and high binding activity against a target substance.
    Type: Grant
    Filed: June 21, 2016
    Date of Patent: January 2, 2018
    Assignee: TAGCYX BIOTECHNOLOGIES
    Inventors: Ichiro Hirao, Michiko Hirao, Shigeyuki Yokoyama
  • Publication number: 20170073683
    Abstract: An object of the present invention is to develop and provide a method for efficiently producing a nucleic acid aptamer, particularly, a DNA aptamer, having higher specificity and binding activity against a target substance than those of nucleic acid aptamers obtained by conventional methods. The present invention provides a transcribable or replicable nucleic acid aptamer comprising a natural nucleotide and a non-natural nucleotide having an artificial base-pairable artificial base. The present invention also provides a method for sequencing a non-natural nucleotide-containing single-stranded nucleic acid molecule selected from a single-stranded nucleic acid library.
    Type: Application
    Filed: November 23, 2016
    Publication date: March 16, 2017
    Applicant: TAGCYX BIOTECHNOLOGIES
    Inventors: Ichiro Hirao, Michiko Hirao, Rie Yamashige, Shigeyuki Yokoyama
  • Patent number: 9540650
    Abstract: An object of the present invention is to develop and provide a method for efficiently producing a nucleic acid aptamer, particularly, a DNA aptamer, having higher specificity and binding activity against a target substance than those of nucleic acid aptamers obtained by conventional methods. The present invention provides a transcribable or replicable nucleic acid aptamer comprising a natural nucleotide and a non-natural nucleotide having an artificial base-pairable artificial base. The present invention also provides a method for sequencing a non-natural nucleotide-containing single-stranded nucleic acid molecule selected from a single-stranded nucleic acid library.
    Type: Grant
    Filed: November 15, 2012
    Date of Patent: January 10, 2017
    Assignee: TAGCYX BIOTECHNOLOGIES
    Inventors: Ichiro Hirao, Michiko Hirao, Rie Yamashige, Shigeyuki Yokoyama
  • Publication number: 20160299131
    Abstract: An object of the present invention is to develop and provide a method for efficiently and conveniently producing a nucleic acid aptamer, particularly, a DNA aptamer, having high specificity for and high binding activity against a target substance.
    Type: Application
    Filed: June 21, 2016
    Publication date: October 13, 2016
    Applicant: TAGCYX BIOTECHNOLOGIES
    Inventors: Ichiro Hirao, Michiko Hirao, Shigeyuki Yokoyama
  • Publication number: 20160289680
    Abstract: An object of the present invention is to develop and provide a method for efficiently and conveniently producing a nucleic acid aptamer, particularly, a DNA aptamer, having high specificity for and high binding activity against a target substance.
    Type: Application
    Filed: June 21, 2016
    Publication date: October 6, 2016
    Applicant: TAGCYX BIOTECHNOLOGIES
    Inventors: Ichiro Hirao, Michiko Hirao, Shigeyuki Yokoyama
  • Publication number: 20140256794
    Abstract: This invention is intended to enhance and improve the resistance of a single- or double-stranded nucleic acid fragment comprising a base sequence of a functional nucleic acid to degradation by nucleolytic enzymes in a simple and cost-effective manner. The single- or double-stranded nucleic acid fragment comprises, ligated to at least one 3? end thereof, a hairpin-shaped DNA comprising: (A) a nucleic acid region consisting of 2 to 5 arbitrary nucleotides; (B) a nucleic acid region consisting of a “gna” or “gnna” base sequence, wherein each “n” represents “g”, “t”, “a”, or “c”, a base analogue, or a modified base; and (C) a nucleic acid region consisting of a base sequence complementary to the nucleic acid region (A), sequentially ligated from the 5? end toward the 3? end, wherein at least one of two 3? terminal nucleotides from at least one 3? end of the single-stranded nucleic acid fragment or the double-stranded nucleic acid fragment is modified.
    Type: Application
    Filed: October 18, 2012
    Publication date: September 11, 2014
    Applicants: RIKEN, TAGCYX BIOTECHNOLOGIES
    Inventors: Ichiro Hirao, Michiko Hirao, Shuang Liu, Iwao Nozawa
  • Publication number: 20130122506
    Abstract: It is an object of the present invention to provide quenching or fluorescent nucleic acid base analogs and applications thereof. The quencher of the present invention has a 2-nitropyrrole structure represented by Formula I: [Formula 1] (in Formula I, R1 and R2 are groups independently selected from the group consisting of: ribose and deoxyribose; hydrogen, hydroxyl and SH groups, and halogens; substituted or unsubstituted alkyl, alkenyl, and alkynyl groups each having 2 to 10 carbon atoms; one or more five-membered heterocyclic rings, one or more six-membered heterocyclic rings, and one or more fused heterocyclic rings, these heterocylic rings containing nitrogen or sulfur, and one or more aromatic rings; sugars, sugar chains, amino acids, and peptides; and fluorescent molecules linked via linkers).
    Type: Application
    Filed: April 21, 2011
    Publication date: May 16, 2013
    Applicants: TAGCYX BIOTECHNOLOGIES c/o RIKEN, RIKEN
    Inventors: Ichiro Hirao, Michiko Hirao, Shigeyuki Yokoyama, Tsuneo Mitsui, Rie Yamashige
  • Publication number: 20110306653
    Abstract: This invention is intended to enhance and improve the resistance of a single- or double-stranded nucleic acid fragment comprising a base sequence of a functional nucleic acid to degradation by nucleolytic enzymes in a simple and cost-effective manner. The single- or double-stranded nucleic acid fragment comprises, ligated to at least one end thereof, hairpin-shaped DNA comprising: (A) a nucleic acid region comprising 2 to 5 arbitrary nucleotides; (B) a nucleic acid region comprising a “gna” or “gnna” base sequence, wherein each “n” represents “g”, “t”, “a”, or “c”, a base analogue, or a modified base; and (C) a nucleic acid region comprising a base sequence complementary to the nucleic acid region (A), sequentially from the 5? end toward the 3? end.
    Type: Application
    Filed: May 13, 2011
    Publication date: December 15, 2011
    Applicants: TAGCYX BIOTECHNOLOGIES, RIKEN
    Inventors: Ichiro HIRAO, Michiko HIRAO, Shuang LIU, Iwao NOZAWA