Abstract: Disclosed herein is a method of labelling a sugar at its reducing end by using 1-(p-methoxyphenyl)-3-methyl-5-pyrazolone, thereby analyzing said sugar with high sensitivity, and to a kit to be used for sugar labelling by this method.
Type:
Grant
Filed:
October 11, 1991
Date of Patent:
August 25, 1992
Assignee:
Takara Shuzo Co., Ltd.
Inventors:
Yuan C. Lee, Susumu Honda, Kazuaki Kakehi
Abstract: Novel spergualin-related compounds represented by the general formula (I): ##STR1## wherein X represents --(CH.sub.2).sub.1-5 -- or a phenylene group which may be substituted; m represents 0, 1 or 2; n represents 1 or 2; and R.sub.1 represents --(CH.sub.2).sub.1-3 --COOH, and pharmacologically acceptable salts thereof, possess an immunopotentiating activity, and are expected to be useful as immunopotentiators applicable to warm blooded animals.
Abstract: An asparaginyl endopeptidase which is specific for only an amide bond on the C-terminal side of an L-asparagine. Also disclosed is a method for the hydrolysis of an amide bond on the C-terminal side of an L-asparagine characterized by the use of an asparaginyl endopeptidase as well as a composition for use in the hydrolysis of an amide bond on the C-terminal side of an L-asparagine.
Abstract: An antibiotic R106 represented by the general formula (I): ##STR1## wherein: R is methyl or ethyl;X.sub.1 is MePhe, .beta.-HOMePhe or Phe;X.sub.2 is allo-Ile, Val or Leu;X.sub.3 is MeVal or Val;X.sub.4 is .beta.-HOMeVal, .gamma.-HOMeVal, MeVal, Val, N,.beta.-MeAsp, .beta.-HOMePhe, MePhe, MeDH.sub.2,3 Val or MeDH.sub.3,4 Valis produced by a process which comprises culturing a strain of the genus Aureobasidium that is capable of producing the said antibiotic R106 and collecting the said antibiotic from the fermentation broth. The antibiotic R106 compounds are useful in the treatment of fungal infection.
Abstract: SP6 bacteriophage RNA polymerase is produced by cultivating a new microorganism (particularly new strains of Escherichia coli) harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene and recovering SP6 bacteriophage RNA polymerase from the culture broth. SP6 bacteriophage RNA polymerase gene is provided as are new microorganisms harboring a plasmid that carries SP6 bacteriophage RNA polymerase gene.
Abstract: The present invention provides a use for the preparation of an immunopotentiator of a spergualin-related compound represented by the following general formula [I] or a pharmacologically acceptable salt thereof: ##STR1## wherein X represents ##STR2## or --(CH.sub.2).sub.n --, Y represents a hydrogen atom or a hydroxyl or hydroxymethyl group,n is an integer of 3 or 5, andR represents --(CH.sub.2).sub.4 --R.sub.1 (wherein R.sub.1 is --NH.sub.2 or --OH), --(CH.sub.2).sub.3 --R.sub.2 (wherein R.sub.2 is --COOH or --CHO), --(CH.sub.2).sub.4 --NH--(CH.sub.2).sub.3 --OH or --(CH.sub.2).sub.4 --NH--(CH.sub.2).sub.2 --R.sub.2 (wherein R.sub.2 is as defined above).
Abstract: Process for purifying spergualin-related compounds and their synthetic intermediates, by subjecting a solution containing such spergualin-related compounds or intermediates to electrodialysis and/or reverse osmosis.
Abstract: A method for the fluorescent labelling of a sugar or a sugar chain by labelling the reducing end of the sugar or sugar chain with a fluorescent substance, wherein the improvement comprises first forming a Schiff base by reacting the surgar or sugar chain with the fluorescent substance in the presence of an acid which is substantially free from water, and second, reducing said Schiff base.
Abstract: NAD(P)H oxidase is disclosed having the following enzymological properties:(1) ActionIt oxidizes NADH or NADPH in the presence of oxygen to form NAD or NAD and hydrogen peroxide.NAD(P)H+H.sup.+ +O.sub.2 .fwdarw.NAD(P).sup.+ +H.sub.2 O.sub.2(2) Substrate specificityIt acts upon NADH and NADPH.(3) Optimum pHIts optimum pH lies in the range of about 9 to 10.Also disclosed is a process for producing the NAD(P)H oxidase and a method for determining the quantity of substrate or enzyme activity in a sample solution by utilizing a reaction system forming NADH or NADPH.
Abstract: The invention provides novel strains of Basidiomycetes and more particularly, novel strains of Lyophyllum ulmarium. The novel strains are characterized by having a cap which is not concave when the fruiting body has matured. A process is provided comprising inoculation of Lyophyllum ulmarium with a cap which is not concave when the fruiting body has matured on a medium to form the fruiting body.Also provided is a method comprising the mating of Lyophyllum ulmarium Lu 1-8 with other Lyophyllum ulmarium and the harvesting of Lyophyllum ulmarium with a cap which is not concave when the fruiting body has matured in a term of cultivation up to harvest of the fruiting body that does not exceed 100 days.
Abstract: A method is provided for the detection of a disease associated with the metabolic abnormality of L-fucose in a subject. The concentration of free L-fucose in a specimen from the subject is determined and the determined concentration is compared with a normal concentration of free L-fucose.
Abstract: A method is provided for the quantitative determination of L-fucose in a sample solution. According to the method an L-fucose dehydrogenase having its optimum pH around neutrality is allowed to act upon the sample solution in the presence or absence of an .alpha.-L-fucosidase, and the amount of reduced nicotinamide adenine dinucleotide thus formed is measured.
Abstract: A novel restriction endonuclease SplI which has the following physicochemical properties:(1) recognizing the following base sequences in double-stranded deoxyribonucleic acid ##STR1## and cleaving said sequences in the phosphodiester bonds between C and G as indicated with the vertical arrows to produce DNA fragments having one strand comprising four bases at the 5'-terminal;(2) cleaving double-stranded deoxyribonucleic acid .lambda.-DNA in one position, Col El in two positions and .phi.x 174 RF in two positions;(3) being activated with 5 to 20 mM Mg.sup.2+ ; and(4) exhibiting an activity at a NaCl concentration of 0 to 200 mM;and a process for the production of the restriction endonuclease SplI which comprises culturing a restriction endonuclease SplI-producing alga belonging to the genus Spirulina, collecting the cells, obtaining a cell-free extract therefrom the separating and purifying the restriction endonuclease SplI.
Type:
Grant
Filed:
July 24, 1985
Date of Patent:
December 12, 1989
Assignees:
Dainippon Ink and Chemicals, Inc., Takara Shuzo Co., Ltd.
Abstract: A restriction enzyme, ApaLI, which recognizes the base sequence in a double-stranded deoxyribonucleic acid molecule as shown below, and cleaves it at the arrow-marked sites, ##STR1## wherein A, G, T and C represent adenosine, guanosine, thymidine and cytidine, respectively. Also provided is a process for producing this enzyme, by growing a microorganism belonging to the genus Acetobacter.