Abstract: The alternate soaking apparatus for treatment of tissue materials has a plurality of chemical liquid containers, a treatment container for soaking and treating a tissue material, a plurality of liquid feed tubes for providing fluid communication between the corresponding chemical liquid containers and the treatment container to introduce the respective chemical liquids into the treatment container, a liquid discharge tube for discharging the chemical liquids in the treatment container, and control means for controllably opening and closing the liquid feed tubes and the liquid discharge tube to allow the plurality of chemical liquids to be contained in the treatment container individually and alternately.