Abstract: The present disclosure relates to methods, processes and systems for enzymatic synthesis of oligonucleotide from a single-stranded, immobilized primer in the presence of a polymerase. Using the disclosed methods single-stranded oligonucleotides can be synthesized enzymatically from a single-stranded, immobilized primer in the presence of deoxyribonucleotide triphosphates or ribonucleotide triphosphates. Dideoxyribonucleotide triphosphates, deoxyribonucleotide triphosphates with reversible terminators, or ribonucleotide triphosphates with reversible terminators can be added enzymatically to the end of the primer or its extension products. According to the disclosed method, a single-stranded primer can bind to a template such that the thus-formed double-stranded structure can allow the polymerase to extend the primer at 3? end.

Abstract: The present disclosure provides methods of sequencing polynucleotides and compounds, compositions useful for sequencing of polynucleotides. The chemical compounds include nucleotides and their analogs which possess a sugar moiety comprising a cleavable chemical group capping the 3?-OH group and a base that is attached to a label through a cleavable linker comprising a disulfide bond. In addition, both the disulfide bond and the cleavable chemical group are cleavable by a chemical reagent. Furthermore, after the disulfide bond is cleaved by the chemical reagent, there is no free thiol group linked to the base of the nucleotides according to the fragmentation reaction shown below as an example. Example compounds according to the present disclosure are shown as Formula (I): wherein w is 1-5; X is O, S, or BH3; B is a nucleotide base or an analog thereof, L1-3 are linkers; and D1 is a label.

Abstract: Provided herein are methods and compositions for the sequencing of long nucleic acids, such as DNA. The methods and compositions are suited for the spatial labeling and sequencing of long nucleic acid molecules.

Abstract: A mutual capacitive touch panel includes a first electrode layer and a second electrode layer. The first electrode layer includes a plurality of first electrode series and a plurality of second electrode series. The second electrode layer includes a plurality of electrode strips, and each electrode strip crosses the first electrode series and the second electrode series. Each first electrode series includes a plurality of first electrodes and a plurality of second electrodes electrically connected with each other, and each first electrode and a corresponding one of the second electrodes are disposed abreast and form an electrode set. Each second electrode series includes a plurality of third electrodes electrically connected with each other, and each electrode set and each third electrode are arranged alternately along a direction.

Abstract: The present invention provides methods and systems for sequencing long nucleic acid fragments. In one aspect of the invention, methods, systems and reagent kits are provided for sequencing nucleic acid target sequences. Some embodiments of the methods, systems and reagent kits are particularly suitable for sequencing a large number of fragments, particularly long fragments.

Abstract: The present disclosure relates to processes for inverting oligonucleotide probes in an in situ synthesized array. These processes can be used to reverse the orientation of probes with respect to the substrate from 3?-bound to 5?-bound. These processes can also be used to reduce or eliminate the presence of truncated probe sequences from an in situ synthesized array.

Abstract: The present disclosure describes systems and methods to construct genomic DNA fragments library by stretching genomic DNA over an adapter-modified chip; using an enzyme cocktail comprising a first restriction enzyme, a ligase, and a second restriction enzyme to cut the genomic DNA into fragments and ligate the fragments to the adapters on the chip; and amplifying thus-obtained DNA by PCR amplification.

Abstract: The methods, systems, kits and reagents provided herein relate to the synchronization of dephased/prephased nucleic acid molecules during sequencing reactions. The methods, systems, kits and reagents provided herein can be utilized to improve the efficiency and accuracy of sequencing technologies.

Abstract: Provided herein are methods and compositions for analyzing epigenetic modifications of genomes. The methods and compositions are suited for complete epigenome sequencing of any modification for which an antibody or an affinity binding agent has been developed. In one aspect, provided herein is a method for analyzing epigenetic modification of a genome. In some embodiments of aspects provided herein, the method further comprises sequencing a sequencing library to generate sequence reads, and assembling the sequence reads with aid of a positional barcode sequence information. In some embodiments of aspects provided herein, the method further comprises determining a location of at least two different epigenetic modifications of the nucleic acid. Another aspect of the present disclosure provides a kit for analyzing an epigenetic modification of a nucleic acid.

Abstract: The present invention provides methods and systems for sequencing long nucleic acid fragment. The present invention also provides a method of sequencing a target polynucleotide with fewer probes. Further, the present invention provides a method of sequencing a target polynucleotide with longer reads. Locus-specific, ligation-assisted sequencing/genotyping method and ligation-captured sequencing method are also provided in the present invention. The methods of the present invention allow low-cost, high-throughput and accurate sequencing of nucleic acids.

Abstract: An apparatus includes a carriage, camera, spray tool, and controller. The carriage is movably coupled to a track. The camera and spray tool are coupled to the carriage. The controller moves the carriage along the track away from a starting position based on a first signal from the camera, and as the carriage moves away from the starting position, discharges, through the spray tool, a solution for a first time. The controller moves the carriage towards the starting position after the solution has been discharged for the first time, and based on a second signal from the camera as the carriage moves towards the starting position and before the carriage reaches the starting position, moves the carriage away from the starting position. As the carriage moves away from the starting position, the controller discharges, through the spray tool, the solution for a second time.

Abstract: The present invention provides methods for targeted sequencing of polynucleotide. In one aspect, the present invention provides a method of sequencing a target polynucleotide with fewer probes. In another aspect, the present invention provides a method of sequencing a target polynucleotide with longer reads. Locus-specific, ligation-assisted sequencing/genotyping method and ligation-captured sequencing method are also provided in the present invention. The methods of the present invention allow low-cost, high-throughput and accurate sequencing of nucleic acids.

Abstract: The present invention relates generally to methods of sequencing of polynucleotides and compounds, compositions and kits useful for sequencing of polynucleotides. The chemical compounds include nucleotide and nucleoside analogs which possess a blocking group covalently attached to the 3? hydroxyl of the sugar moiety. The blocking group may optionally be additionally covalently attached to a linker and/or detectable label. The nucleotide analogs may be ribonucleotide or deoxyribonucleotide analogs. Methods include incorporation of the reversible terminator molecules into growing polynucleotide strands by polymerase enzymes, such as in single base sequencing methodologies utilizing sequential reversible termination techniques.

Abstract: The present invention provides methods and systems for sequencing long nucleic acid fragments.

Abstract: A milking robot comprising a movable arm and a plurality of grippers. The movable arm has a length in a longitudinal direction. The plurality of grippers are swivably mounted to the movable arm such that each gripper moves between a first position parallel to the movable arm's longitudinal direction and a second position at which the gripper extends sideways in a horizontal plane away from the movable arm's longitudinal direction.

Abstract: Milking box or boxes for cows, each milking box having two entrance fences for entry and two exit fences, wherein one first entrance fence and one first exit fence form a first pair of fences positioned at a first side, and one second entrance fence and one second exit fence form a second pair of fences at a second side which is opposite the first side. This type of milking box used in a cow stable further comprises resting boxes, at least one feeder trough, a separation zone or zones, and walking paths, wherein at least the resting boxes are placed at the first side of the milking box or boxes, and that at the second side of the milking box or boxes at least one is placed selected from the group comprising at least one feeder trough, a separation zone or zones, or outdoors.

Abstract: The present invention relates to a robot (6) for moving a milking cup or milking cups (7) from a first resting position to a second operational position or vice versa, in which second position it connects to a cows' teat or teats for collecting milk, wherein it comprises a frame (9), which frame supports a movable arm (10) that has a free extremity (11) at which it is provided with a gripper or grippers (12) for the milking cup or cups (7).

Abstract: Milking box or boxes for cows, each milking box having two entrance fences for entry and two exit fences, wherein one first entrance fence and one first exit fence form a first pair of fences positioned at a first side, and one second entrance fence and one second exit fence form a second pair of fences at a second side which is opposite the first side. This type of milking box used in a cow stable further comprises resting boxes, at least one feeder trough, a separation zone or zones, and walking paths, wherein at least the resting boxes are placed at the first side of the milking box or boxes, and that at the second side of the milking box or boxes at least one is placed selected from the group comprising at least one feeder trough, a separation zone or zones, or outdoors.

Abstract: An apparatus and method of conditioning humidity and temperature in the process air stream of a desiccant dehumidifier used to dry moisture-laden spaces and structures by replacing moisture-laden air with dehumidified air to increase the rate of water evaporation within the affected areas. An air-dehumidifier comprises a dehumidification assembly having a desiccant rotor assembly, a shielded radiant burner assembly, and a control system. The shielded radiant burner assembly is used to regenerate the desiccant rotor by removing moisture from the reactivation quadrant, where high velocity (at least 500 ft/min) air streams are often flowed, by projecting radiant heat onto the rotor, while minimizing the potential for the high velocity air streams interrupting the combustion of air and fuel in the radiant burner.

Abstract: A novel soybean cultivar, designated DP 5634 RR, is disclosed. The invention relates to the seeds of soybean cultivar DP 5634 RR, to the plants of soybean DP 5634 RR and to methods for producing a soybean plant produced by crossing the cultivar DP 5634 RR with itself or another soybean variety. The invention further relates to hybrid soybean seeds and plants produced by crossing the cultivar DP 5634 RR with another soybean cultivar.