Abstract: A method of expanding and maintaining human embryonic stem cells (ESCs) in an undifferentiated state by culturing the ESCs in a suspension culture under culturing conditions devoid of substrate adherence is provided. Also provided are a method of deriving ESC lines in the suspension culture and methods of generating lineage-specific cells from ESCs which were expanded in the suspension culture of the present invention.
Type:
Grant
Filed:
November 16, 2017
Date of Patent:
April 6, 2021
Assignee:
Teehnion Research & Development Foundation Limited