Abstract: The invention relates to a biological indicator for determining the efficacy of a steam or heat sterilization process, and its method. The biological indicator comprises microbial spores (a), at least one sensor protein exogenous to the microbial spores (b), one fluorophore (c), and a culture medium (d). The invention also refers to the method of use of this biological indicator.

Abstract: A reactive ink composition comprising a UV-C radiation sensitive polymer, an acid scavenger, a photoinitiator, and a pH-sensitive dye. A method to print a composition onto a UV-C radiation dosimeter using a “layer-by-layer” deposition technique, as well as a dosimeter comprising the reactive ink composition, useful in monitoring the efficiency of a UV-C radiation sterilization or disinfection process.

Abstract: A biological indicator for testing the efficacy of sterilization or disinfection processes of closed environments or confined spaces, the biological indicator comprising a carrier; biological material supported on or embedded in the carrier; and a carrier container comprising a body and a cap. The carrier is fixed within an inner space of the cap, the cap being able to be placed in an open position completely exposing the carrier and the biological material to the closed environment or confined space to be disinfected. A method for determine the efficacy of a process of sterilization or disinfection of closed environments or confined spaces, preferably by means of an enzyme and a fluorescence compound, using said biological indicator.