Abstract: A centrifugable sample carrier (1) is provided having a liquid reservoir (2) which is closed with a sealing film (7). The sample carrier (1) has at least one deformable region (8), and the liquid reservoir (2) is openable by a deformation of the deformable region (8). The deformable region (8) has at least one activation element (10), which pierces the sealing film (7) in the event of a deformation of the deformable region (8).

Abstract: A method (7) for detecting living microorganisms (1) and/or for differentiating between living (12) and dead (13) microorganisms in a sample (9) by in-situ hybridization (14), especially fluorescence in-situ hybridization, and optical analysis (26) of the microorganisms (1) to be detected, wherein at least one substance (10) is added to the sample (9) before the hybridization (14), which substance (10) promotes a differing alteration of the RNA concentration in dead cells and in living cells.

Abstract: An oligonucleotide probe combination (1) for the specific detection of microorganisms having a primary probe (5) and a secondary probe (6), wherein the primary probe (5) has at least one functional section (2) and the secondary probe (6) at least one tag (3) generating a detectable signal. The functional section (2) binds specifically to a target sequence of a microorganism to be detected, and the primary probe (5) and the secondary probe (6) are linked to one another but constitute separate chains (8).

Abstract: A centrifugable sample carrier (1) having a fluidic system (2), the fluidic system having at least one chamber (3, 20, 21, 32, 44, 45, 46, 63) which has at least one inflow opening 4, the chamber (3, 20, 21, 32, 44, 45, 46, 63) having at least two mutually spaced-apart ventilation openings (6, 7), and the ventilation openings (6, 7) being mutually disposed in such a way that a connecting line between the ventilation openings (6, 7) does not run through the chamber (3, 20, 21, 32, 44, 45, 46, 63). A sample carrier (1) of this type is particularly suitable for use in analytical methods for detecting microorganisms, but is not restricted to this use.

Abstract: A detection method (1) for antibiotic-resistant microorganisms (2), in which at least one specific substance (3) which is broken down by an antibiotic resistance-causing enzyme (8) of the microorganism (2) is added to the sample (5), the enzyme (8) present triggers a reaction (4), and the reaction (4) involves generation of an optically detectable reaction product (6) near the resistant microorganism (2) that is subsequently detected in an optical detection method (7).

Abstract: A method in a detection area (2) for recording microparticles (1), wherein the microparticles (1) are collected on a membrane (3) and recorded by a sequence (4) of recordings (5) along the membrane (3), and at least one reference marker (6) is recorded per recording (5).

Abstract: A rotary plate (1) of a centrifuge (2) having a pivoting plate (3) mounted thereon is provided, to carry out a rotational and/or pivoting movement of the pivoting plate (3) relative to the rotary plate (1). This allows the rotary plate (1) to be drivable by a drive (5) rotationally decoupled from the rotary plate (1).

Abstract: A process for recording microparticles (1) in a receiving space (3) is provided, with the microparticles (1), preferably in a fluid (4), being transferred into the outwardly closed receiving space (3) prior to the optical recording, and the receiving space (3) being outwardly opened prior to the recording. Such a process and receiving space (3) are of interest in particular for the recording and analysis of microparticles (1) in pharmacy and the foodstuff industry, but are not restricted to these sectors of industry.

Abstract: An oligonucleotide probe combination (1) for the specific detection of microorganisms having a primary probe (5) and a secondary probe (6), wherein the primary probe (5) has at least one functional section (2) and the secondary probe (6) at least one tag (3) generating a detectable signal. The functional section (2) binds specifically to a target sequence of a microorganism to be detected, and the primary probe (5) and the secondary probe (6) are linked to one another but constitute separate chains (8).

Abstract: What is proposed is to provide a method for detecting microorganisms, for example of the Enterobacteriaceae family of bacteria, in a sample by means of delivery of identity-determining nucleic acid probes into the individual cell bodies, wherein the nucleic acid probes hybridize to the nucleic acids of the microorganisms, and subsequent optical detection of the hybridizations generated in the individual cell bodies, wherein a mixture of at least a first nucleic acid probe and a second nucleic acid probe is used, and wherein the first nucleic acid probe and the second nucleic acid probe respectively bind in one of two nonoverlapping regions of the target nucleic acid.

Abstract: A method (7) for detecting microorganisms (1) in a sample (9), by delivery (10) of specific nucleic acid probes (11) into the individual microorganisms (1), which react (12) with the nucleic acid material present in the microorganisms (1), and subsequent optical detection (16) of the reaction products generated in the individual microorganisms (1), in which fixation (13) of the microorganisms (1) contained in the sample (9) is carried out before the delivery (10) of the nucleic acid probes (11) and a detergent (14) is added to the sample (9) before the fixation step (13) is completed.

Abstract: A probe complex (1) is provided for the specific detection of at least one substance (2), wherein the probe complex (1) binds specifically to a target segment (7) of the substance (2) and wherein an interaction with at least one detectable tag (8) can trigger a preferably optically detectable signal. The probe complex (1) includes at least one probe type in the form of a primary probe (6) having a target-specific segment (5) and at least one further probe type in the form of a secondary probe (9) having a target-specific segment (10), wherein the target-specific segments of all probe types of the probe complex (1) are matched to one another in such a way that a chain (3) of at least three probes (4) can be formed.

Abstract: A detection method (1) for antibiotic-resistant microorganisms (2), in which at least one specific substance (3) which is broken down by an antibiotic resistance-causing enzyme (8) of the microorganism (2) is added to the sample (5), the enzyme (8) present triggers a reaction (4), and the reaction (4) involves generation of an optically detectable reaction product (6) near the resistant microorganism (2) that is subsequently detected in an optical detection method (7).

Abstract: A method (7) for detecting living microorganisms (1) and/or for differentiating between living (12) and dead (13) microorganisms in a sample (9) by in-situ hybridization (14), especially fluorescence in-situ hybridization, and optical analysis (26) of the microorganisms (1) to be detected, wherein at least one substance (10) is added to the sample (9) before the hybridization (14), which substance (10) promotes a differing alteration of the RNA concentration in dead cells and in living cells.