Abstract: The patent application relates to isolated polypeptides that specifically cleave non-glycosidic ether bonds between lignins or derivatives thereof and saccharides, and to cDNAs encoding the polypeptides. The patent application also relates to nucleic acid constructs, expression vectors and host cells comprising the cDNAs, as well as methods of producing and using the isolated polypeptides for treating pulp and biomass to increase soluble saccharide yield and enrich lignin fractions.
Abstract: A method for biotransformation of organic compounds using non-prokaryotic microalgae is disclosed. The method is useful to biotransform a chemical precursor compound, preferably a heterocyclic compound, to a chemically distinct final product, which is useful in, e.g., pharmaceutical, agrichemical, nutraceutical, ecological, hazardous waste, food flavoring, or food additive applications.