Abstract: A composition and a method for analyzing an anti-aging effect of the composition of a fecal derived sterilized postbiotic are disclosed. The method includes obtaining a baseline biomarker of aging measurement, wherein the baseline biomarker of aging measurement includes a biomarker of aging, selecting a dose schedule from a plurality of dose schedules of a composition for a fecal derived sterilized postbiotic as a function of the baseline biomarker of aging measurement, wherein the composition includes a plurality of biological macromolecules including at least a short chain fatty acid, wherein the short chain fatty acid includes acetic acid, propionic acid, and butyric acid and a plurality of agents, wherein the plurality of agents is configured to regulate the biomarker of aging and administering the composition to a subject.

Abstract: Methods and compositions relating to sterilized fecal microbiota and compositions derived from the same. A method of sterilizing a fecal composition to obtain a sterilized fecal composition is provided. Methods of further purifying the sterilized composition and optionally freezing or freeze-drying the compositions is also provided. Methods of using the compositions for treatment in beverages, foods, and in supplements are also provided.