Abstract: The cystic fibrosis gene and its gene product are described for both the normal and mutant forms. The genetic and protein information is used in developing DNA diagnosis, protein diagnosis, carrier and patient screening, drug and gene therapy, cloning of the gene and manufacture of the protein, and development of cystic fibrosis affected animals.

Abstract: The cystic fibrosis gene and its gene product are described for both the normal and mutant forms. The genetic and protein information is used in developing DNA diagnosis, protein diagnosis, carrier and patient screening, drug and gene therapy, cloning of the gene and manufacture of the protein, and development of cystic fibrosis affected animals.

Abstract: The cystic fibrosis gene and its gene product are described for both the normal and mutant forms. The genetic and protein information is used in developing DNA diagnosis, protein diagnosis, carrier and patient screening, drug and gene therapy, cloning of the gene and manufacture of the protein, and development of cystic fibrosis affected animals.

Abstract: A heat and shelf-stable oil-in-water emulsion useful as a tissue or cell-selective delivery vehicle. Radioactive or stable, synthetic or semi-synthetic polyhalogenated triglycerides, such as 2-oleoylglycerol-1,3-bis[7-(3-amino-2,4,6-triiodophenyl)heptanoate] or 2-oleoylglycerol-1,3-bis[.omega.-(3,5-bis-trifluoromethyl)-heptanoate] or phenyl acetate, can be incorporated into the lipophilic core of a lipoprotein-like emulsion particle. The lipophilic core is surrounded by a phospholipid membrane comprising cholesterol and apolipoproteins. For hepatocyte-selective delivery, the emulsion is chylomicron remnant-like by being in a size range of 50 to 200 nm as measured by number weighting analysis with a narrow size distribution (<2% greater than 300 nm) and having a composition simulating naturally-occurring chylomicron remnants. Use of cholesterol in the emulsion formula facilitates association of apolipoproteins, especially Apo E which are recognized by liver cells and necessary for binding and uptake.

Abstract: The cystic fibrosis gene and its gene product are described for both the normal and mutant forms. The genetic and protein information is used in developing DNA diagnosis, protein diagnosis, carrier and patient screening, drug and gene therapy, cloning of the gene and manufacture of the protein, and development of cystic fibrosis affected animals.

Abstract: A device, and process, for continuously separating dissolved solutes and suspended particles from a multicomponent fluid phase in a magnetically stabilized fluidized bed (MSFB). The process is of the type wherein a solid resin and a liquid buffer move countercurrent to each other in the bed. In steady state operation, a time invariant pH gradient will form in the bed and remain spatially fixed. Other condition variables, such as temperature, can be used to form a time invariant gradient provided that equilibrium partition between the solid and liquid phases is a function of the condition variable.

Abstract: A polymeric membrane electrode gives potentiometric responses to macromolles, such as protamine, in solution or biological specimens. A protamine-responsive membrane includes a lipophilic cation-exchange material for ionic macromolecules in a specially formulated plasticized, polymeric matrix material. Potentiometric response to protamine has been observed in membranes comprising 30-70 wt. % polymeric matrix material, such as polyvinyl chloride; 1-3 wt. % salt of a tetraphenylborate, such as potassium tetrakis(4-chlorophenyl) borate or salt of an organophosph(on)ate, such as calcium bis-[4-(1,1,3,3-tetramethylbutyl)phenyl] phosphate; and 30-70 wt. % of a plasticizer, such as 2-nitrophenyl octyl ether or tris (2-ethylhexyl) phosphate.

Abstract: Water-soluble, high-viscosity grade cellulose ether compositions are useful for the reduction of serum lipid levels, particularly total serum cholesterol, serum triglycerides, and low-density lipoprotein (LDL) levels and/or attenuate the postprandial rise of blood glucose levels in animals. The pharmaceutical uses of compositions for reducing cholesterol levels additionally include the treatment of diabetes and/or hypercholesterolemia, with avoidance of many of the undesirable side effects associated with other forms of treatment. The composition may be in the form of a prehydrated ingestible composition, such as a gelatin, or a comestible, such as a cookie. In addition, the compositions of the present invention are palatable to the patient, thereby enhancing patient compliance with the regimen.

Abstract: Less toxic agents for reversal of heparin or low molecular weight heparin anticoagulation which are synthetic protamine-like polycationic peptides having a total cationic charge which is less than that of n-protamine. In preferred embodiments, arginine residues of n-protamine are replaced with lysine residues for ease of manufacture. Selective positively charged arginine residues have been replaced with an uncharged amino acid residue or its analog, such as glycine or glutamine, in order to reduce the total cationic charge on the polycationic peptide to the range of about [+14] to [+18], preferably [+16]. In specific embodiments, there are sequences of 29 amino acid residues wherein 4 to 5 clusters of 2 to 4 positively charged amino acids are separated by 2 to 6 neutral amino acids. The C-terminus and the N-terminus can be modified to mitigate against in vivo degradation by carboxypeptidases and aminopeptidases.

Abstract: A generic cell controlling method and apparatus for a computer integrated manufacturing system accepts manufacturing operation commands to perform a selected manufacturing operation on a selected manufacturing tool. The sequence of generic steps to be performed by the manufacturing tool is determined, in order to implement the selected manufacturing operation. Each step is used to generate operational instructions for the tool controller. The sequence of generic steps is preferably produced using a database which has an entry for each manufacturing operation. Each entry includes parameters which are specific for each tool, and an indication of sequence of steps to be performed. When a manufacturing operation command is received, the command is matched to a database entry and the parameters and sequences associated with that entry are used to call a series of routines or modules. Associated parameters are provided to each routine for generating the operational instructions for the particular tool.

Abstract: Novel difunctionalized cyclobutabenzene monomers of the general formula: ##STR1## wherein Z can be hydrogens or a cyclobutane ting; and X and Y are carbox amino, alcohol, isocyanate, acid halide, or bis-acyl fluoride groups. In a particularly preferred embodiment, the cyclobutabenzene derivative is 1,2-dihydrocyclobutabenzene-3,6-carboxylic acid. The difunctionalized cyclobutabenzene monomer can form part of a polymer backbone chain, but has an additional functionality, the butane ring, which can be easily opened to produce strong, covalent bond crosslinking between polymer chains. The crosslinking can be induced simply by heating the polymer to a temperature in excess of 300.degree. C.

Abstract: Soluble alkyl [5-(amino (phenyl)methyl)-2H-benzimidazol-2-yl]carbamates, their enantiomorphic forms, and acid addition salts thereof are well absorbed and possess curative anthelmintic activity, especially against filarial worms, when administered orally or parenterally. Pharmaceutical compositions containing the compounds and methods of employing the compounds in methods of treating helminth infections in mammals are also disclosed, together with methods of synthesis.

Abstract: A thermopile detector is disclosed consisting of a semiconductor supporting rim which is doped across all of the rim. The rim supports a series of polycrystalline silicon and metal thermocouples. The fully doped semiconductor area serves as an etch stop for a single-sided etch which eliminates the need for front-to-back alignment of the device. The semiconductor doped rim also serves as a good thermal conductor for supporting the cold junctions. The hot junctions of the thermocouples may be supported by a thin dielectric membrane spanning the device and the cold junctions are formed on the doped rim. The thin dielectric window provides thermal isolation between the semiconductor rim and the center of the window where the hot junctions are located. The thermocouple material layers may be stacked to enable greater thermocouple density on the device. Refractory metals may be employed as the thermocouple metal, to increase sensitivity.

Abstract: A thermopile detector is disclosed consisting of a semiconductor supporting rim which is doped across all of the rim. The rim supports a series of polycrystalline silicon and metal thermocouples. The fully doped semiconductor area serves as an etch stop for a single-sided etch which eliminates the need for front-to-back alignment of the device. The semiconductor doped rim also serves as a good thermal condutor for supporting the cold junctions. The hot junctions of the thermocouples may be supported by a thin dielectric membrane spanning the device and the cold junctions are formed on the doped rim. The thin dielectric window provides thermal isolation between the semiconductor rim and the center of the window where the hot junctions are located. The thermocouple material layers may be stacked to enable greater thermocouple denisty on the device. Refractory metals may be employed as the thermocouple metal, to increase sensitivity.

Abstract: A process for relieving pain and novel topical compositions for transdermal delivery of a narcotic analgesic comprising the application to mammalian skin of an effective amount of a compound of the formula: ##STR1## or a pharmacologically acceptable acid addition salt thereof, where R is --CH.sub.2, ##STR2## or --H and R' and R" are C.sub.1 to C.sub.6 hydrocarbons or --H, in association with a topical pharmaceutical carrier selected from the group consisting of ointments, lotions, pastes, jellies, gels, creams, sprays, aerosols and the like.

Abstract: Novel partially stabilized zirconia ceramic compositions exhibiting improved toughness comprising: a major component consisting of ZrO.sub.2, a minor stabilizing component Y.sub.2 O.sub.3 and an effective amount of a toughening agent (e.g., Ta.sub.2 O.sub.5). The toughness of the tetragonal zirconia polycrystal (e.g., 96% ZrO.sub.2, 3 mole % Y.sub.2 O.sub.3) can be increased nearly three-fold by replacing 1% of the ZrO.sub.2 with Ta.sub.2 O.sub.5. Empirical equations based on ratios of fundamental lattice parameters (e.g. c/a) quantitatively relating to the stability/instability of tetragonal zirconia oxide are disclosed and used in a novel method for improving the toughness of the ceramic compositions.

Abstract: An improved method of inducing a change in the dynamic torque transmission of an electrorheological fluid in response to an electric field at low current comprising the steps of: (a) selecting a nonconductive liquid phase (e.g. high dielectric hydrocarbon oil); (b) dispersing in the nonconductive liquid phase a particulate phase substantially free of adsorbed water (e.g. zeolite); and (c) subjecting the resulting electrorheological fluid to an electric potential in excess of about one kilovolt at a current density of less than about one-third microamp per square inch. Such a method achieves a rate of increase of dynamic transmission of torque per unit voltage, dM/dV, in excess of about 0.034 ft-lbs.times.10.sup.-3 /kV and a ratio of dynamic transmission of torque at an electric field strength of at least 2.4 kV to the dynamic transmission of torque at zero field strength, M/Mo, of at least about 2.

Abstract: A method of growing an epitaxial interface comprising the steps of select a crystalline substrate (e.g. Si) characterized by a crystal lattice substantially similar to the crystal lattice to be grown (e.g. CuCl); applying a magnetic field to the crystalline substrate; and growing a crystal layer on the selected substrate in the presence of a magnetic field. Preferably the crystal growth takes place from a liquid phase eutectic mixture (e.g. CuCl/NH.sub.4 Cl) at conditions near the eutectic point. The epitaxial interface produced is very reproducible, uniform and stable, yet the region appears to be highly strained due to the lattice mismatch. Anomalous superdiamagnetism, very low electrical resistivity and the ability to trap and channel light (index of refraction approaching infinity) are observed at the epitaxial interface at essentially ambient conditions suggestive of high temperature superconductivity.

Abstract: A facile synthesis of the precursor, Cp.sub.2 Mo.sub.2 (.mu.-S).sub.2 (.mu.-SH).sub.2, to novel heterobimetallic cluster compositions (e.g., Cp.sub.2 Mo.sub.2 Fe.sub.2 (.mu.-S).sub.2 (CO).sub.8 ; Cp.sub.2 Mo.sub.2 Ni.sub.2 (.mu..sub.3 -S).sub.4 (CO).sub.2 ; Cp.sub.2 Mo.sub.2 Co.sub.2 (.mu..sub.3 -S).sub.2 (.mu..sub.4 -S)(CO).sub.4 ; Cp.sub.2 Mo.sub.2 Fe.sub.2 (.mu..sub.3 -S).sub.4 (CO).sub.6 and Cp.sub.4 Mo.sub.2 Ni.sub.2 S.sub.4 and the subsequent synthesis and use of the heterobimetallic cluster compositions as highly active and selective catalysts for the hydrogenation of carbon monoxide. Such heterobimetallic cluster catalysts supported on alumina exhibit extraordinary activity and selectivity with respect to the formation of ethane without concommitant formation of C.sub.3, C.sub.4 and heavier hydrocarbons. The catalysts are contemplated as being useful for syngas conversion even in the presence of several ppm of H.sub.2 S or the like in the feedstream.

Abstract: A photothermal deflection detector for thin layer chromatography includes a translation means for holding and moving a thin layer chromatographic plate; a first impinging laser beam (e.g., 20 mW chopped argon laser) focused on the moveable thin layer chromatographic plate; a second probing laser beam (e.g., 2 mW He-Ne laser) intersecting the impinging laser beam directly above and parallel to the moveable thin layer chromatographic plate; and a laser deflection measuring means (e.g., knife edge, photodiode detector and lock-in amplifier demodulator) responsive to the thermal lens effect created by the absorption of the impinging laser beam by the separated compounds on the thin layer chromatographic plate. Such a system when applied to separated compounds (e.g., 1,2-napthaquinone, phenanthrenequinone and .alpha.-ionone) exhibits detection limits that range from 30 ng to 7.5 pg, depending upon the compounds's ability to absorb the impinging light.