Abstract: In certain aspects, the present invention provides methods for inducing a stable gene modification of a target nucleic acid via homologous recombination in a primary cell, such as a primary blood cell and/or a primary mesenchymal cell. In certain other aspects, the present invention provides methods for enriching a population of genetically modified primary cells having targeted integration at a target nucleic acid. The methods of the present invention rely on the introduction of a DNA nuclease such as a Cas polypeptide and a homologous donor adeno-associated viral (AAV) vector into the primary cell to mediate targeted integration of the target nucleic acid. Also provided herein are methods for preventing or treating a disease in a subject in need thereof by administering to the subject any of the genetically modified primary cells or pharmaceutical compositions described herein to prevent the disease or ameliorate one or more symptoms of the disease.

Abstract: The invention provides an immunoconjugate of formula: or pharmaceutically acceptable salt thereof, wherein subscript r is an integer from 1 to 10, subscript n is an integer from about 2 to about 25, and “Ab” is an antibody construct that has an antigen binding domain that binds HER2. The invention further provides compositions comprising and methods of treating cancer with the immunoconjugate.

Abstract: Described herein, inter alia, are compositions and methods for modulating mu opioid receptor activity.

Abstract: A metasurface optical device composed of three stacked dielectric layers which form an anti-reflective structure for wavelengths in a predetermined operational wavelength range within the visible spectrum. The anti-reflective structure contains a rectangular lattice of rhombohedral perturbations that produce guided-mode resonances within the predetermined operational wavelength range. The guided-mode-resonant dielectric metasurface device is capable of detecting by colorimetric readout the presence and orientation of a linearly birefringent anisotropic medium, such as a fibrous tissue, positioned above the stacked dielectric layers.

Abstract: A collector device of environmental exposure is provided. This device may be used to collect and, after technical upgrade, monitor environmental exposure in personal and stationary settings. By coupling with advanced genomic analysis and chemical analysis technologies, the device and its accompanying methodology are capable of detecting environmental agents of diverse nature, many of which could pose health risks if going unaware of or uncontrolled. This type of information provides much needed clues to reconstruct and pinpoint the course of disease etiology at both personal and epidemic scales. By combining personal exposome and personal omics analyses, we can recapitulate with the intention to then prescribe treatment plans with unprecedented precision.

Abstract: Modular lens adapter system are provided for use with a hand held computer device having a camera for mobile ophthalmoscopy. The modular lens adapter systems include an anterior adapter and a posterior adapter configured to removably engage with a hand held computer device. The anterior adapter can include a variable intensity light source, a clamp, and a movable macro lens. The posterior adapter can include a hand held computer device mount, a lens mount, and a telescoping arm with a first end and a second end with the first end configured to removably engage with the mount and the second end configured to contact the lens mount. Methods are also provided for using the systems to obtain an image of an anterior portion of the patient's eye and a posterior portion of the patient's eye.

Abstract: Methods of treatment for menstrual complications, gestational complications, and to prolong gestation are described. Treatments include administration of a compound related to regulation of gestational progress or uterine contractions.

Abstract: Processes and materials to detect cancer from a biopsy are described. In some cases, cell-free nucleic acids can be sequenced, and the sequencing result can be utilized to detect sequences derived from a neoplasm. Detection of somatic variants occurring in phase can indicate the presence of cancer in a diagnostic scan and a clinical intervention can be performed.

Abstract: System, methods, and other embodiments described herein relate to improving the cycling of batteries by using data and a hierarchical Bayesian model (HBM) for predicting the cycle life of a cycling protocol. In one embodiment, a method includes classifying cycle life of a battery into a class using battery data from cycling with a protocol, wherein the class represents cycle life distributions of cycling protocols. The method also includes quantifying, using the class in a HBM, variability for the battery induced by the protocol. The method also includes predicting, using the HBM, an adjusted cycle life for the protocol according to the variability. The method also includes communicating the adjusted cycle life to operate the battery.

Abstract: The disclosure relates to the treatment of primary and metastatic cancer using radiation. Specifically, the disclosure relates to methods providing for the selective accretion of cytoprotective agent in tissues and/or organs, sensitive to radiation that are adjacent to malignant tumors prior to radiation of the tumors at a dose that otherwise would be toxic to the tissues and/or organs, but are necessary to achieve ablative outcome on the tumors.

Abstract: A backscatter tag device includes, in part, a receiver configured to receive a packet conforming to a communication protocol defining a multitude of codewords, a codeword translator configured to translate at least a first subset of the multitude of codewords disposed in the packet to a second multitude of codewords defined by the protocol in response to a data the backscatter tag is invoked to transmit, and a transmitter configured to transmit the packet supplied by the codeword translator at a frequency different than the first frequency at which the packer is received. The communication protocol may optionally be the 802.11 g/n, ZigBee or the Bluetooth communication protocol.

Abstract: A handheld device for sonifying electrical signals obtained from a subject is provided. The device can utilize at least one of several operations including (but not limited) digitizing signals from electrodes, adjusting the signals based on accelerometer input, filtering the signals, conditioning the signals according to conditioning parameters, modulating the signal according to sound synthesis parameters, and generating sound from the representations of the signals to accomplish sonification. The device can include an analog-to-digital (A/D) converter to digitize the one or more electrical signals and a processor that receives the one or more digitized electrical signals and produces a representation of an acoustic signal. The device further includes a speaker system that sonifies the representation of the acoustic signal.

Abstract: The invention is directed to methods of treating TNBC in a patient by administering to the patient an agent that inhibits the expression or activity of cyclin-dependent kinase 19 (CDK19). In some embodiments, the agent may be a small molecule inhibitor, a polynucleotide (e.g., shRNA. siRNA), or a protein (e.g., an antibody). In some embodiments, the agent does not inhibit the activity or expression of CDK8.

Abstract: Methods are provided for treating a subject with an anti-CD47 agent.

Abstract: The present disclosure provides methods and compositions for generating populations of auxotrophic cells and populations of differentiated cells and selecting populations of transfected cells using split auxotrophy.

Abstract: Improved efficiency for nonlinear optical interactions is provided by using strongly confining waveguides for simultaneous imposition of dispersion design constraints at two or more dispersion orders. Quasi-phase-matching allows for phase-matching to be accomplished independently of the waveguide design, which helps provide sufficient design freedom for the dispersion design.

Abstract: A method comprises a client device receiving a verification request comprising an interaction identifier and generating a random sampling of a number of block headers. The random sampling according to a random distribution generated based on a probability density function. The client device can query two or more full nodes for random samplings of block headers. The client device can receive the random samplings of block headers from the two or more full nodes and compare samplings of block headers received from two or more full nodes. The samplings of block headers are the random samplings of block headers. The client device can verify at least one block header of the samplings of block headers, then determine that a blockchain maintained by at least one of the two or more full nodes is valid in response to verifying the at least one block header of the samplings of block headers.

Abstract: This disclosure provides, among other things, a reagent system for nucleic acid analysis. In some embodiments, the system may comprise a plurality of oligonucleotide sets each set comprising at least (a) a competitor oligonucleotide that hybridizes to a target sequence and varies in concentration from mixture to mixture and (b) a detector oligonucleotide that also hybridizes to the target sequence and contains a barcode that indicates the concentration of the competitor oligonucleotide in the oligonucleotide set. The reagent system may be used to analyze a nucleic acid sample.

Abstract: Disclosed are knottin peptides containing non-natural amino acids so that they can be formed by chemical conjugation into two or more knottin monomers. The knottin monomers comprise a non-natural amino acid such as an aminooxy residue within the polypeptide sequence. The exemplified dimers were produced by oxime formation between two aldehyde groups present on a polyether linker and an aminooxy functional group that was site-specifically incorporated the knottin. Knottins variants based on EETI (Ecballium elaterium trypsin inhibitor) and AgRP (Agouti-related protein) were engineered to contain integrin-binding loops. These dimers were shown to have increased binding strength to integrins on U87MG tumor cells, achieving significant increases in inhibition of cell adhesion and proliferation. Also disclosed are knottin monomers comprising an aminooxy residue; these may be conjugated to molecules such as doxorubicin.

Abstract: A photonic crystal optical phased array device has a dispersion engineered slow light waveguide region; a mode coupler region capable of optically coupling an input waveguide to the dispersion engineered slow light waveguide region; and optical antenna regions integrated within the dispersion engineered slow light waveguide region. The dispersion engineered slow light waveguide region has a substantially linear dispersion relation within a predetermined operational bandwidth of the optical phased array device. The optical antenna regions are formed by an alteration of a periodic structure of the photonic crystal and are capable of radiating light out from the dispersion engineered slow light waveguide region.