Abstract: A method for analyzing short tandem repeats (STRs) is described herein. In some embodiments, the method comprises: separately digesting two portions of a genomic sample at sites that are upstream and downstream of an STR; fragmenting those products; ligating adaptors to the fragmentation products; selectively amplifying part of the top strand but not the bottom strand of the ligation products derived from the first portion, and part of the bottom strand but not the top strand of the ligation products derived from the second portion; sequencing at least some of the amplification products to produce a plurality of top strand reads and a plurality of bottom strand reads; and counting the number of STR repeats in a sequence read. A kit for performing the method is also provided.
Type:
Grant
Filed:
June 8, 2016
Date of Patent:
November 5, 2019
Assignee:
THE BOARD OF TRUSTEES OF THE LELEAND STANFORD JUNIOR UNIVERSITY
Inventors:
Giwon Shin, Billy Tsz Cheong Lau, HoJoon Lee, Hanlee P. Ji
Abstract: Recombinant adeno-associated viral (AAV) capsid proteins are provided. Methods for generating the recombinant adeno-associated viral capsid proteins and a library from which the capsids are selected are also provided.
Type:
Grant
Filed:
August 24, 2012
Date of Patent:
October 27, 2015
Assignee:
THE BOARD OF TRUSTEES OF THE LELEAND STANFORD JUNIOR UNIVERSITY