Patents Assigned to The European Molecular Biology Laboratory
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Patent number: 11926862Abstract: The present invention relates to the field of therapeutics and, more in particular, to pharmaceutical compositions for the prevention and/or treatment of bacterial infections and antibacterial-induced dysfunctions. The compositions of the present invention demonstrate high species-specificity in inhibiting the growth of a small number of bacterial species, and most importantly are effective also against multi drug resistant (MDR) clinical isolate species. Interestingly, one of those combinations pairs a non-antibiotic drug, vanillin, with an antibiotic drug, spectinomycin, to demonstrate a surprisingly strong inhibitory effect on the growth of clinically relevant Gram-negative pathogenic and multi-drug resistant E. coli isolates. A second set of compounds combines the polymyxin colistin with loperamide, a rifamycin, or a macrolide.Type: GrantFiled: April 17, 2019Date of Patent: March 12, 2024Assignee: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Athanasios Typas, Ana Rita Gontao Brochado, Stephan Göttig
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Patent number: 11892945Abstract: A method for storage of an item of information (210) is disclosed. The method comprises encoding bytes (720) in the item of information (210), and representing using a schema the encoded bytes by a DNA nucleotide to produce a DNA sequence (230). The DNA sequence (230) is broken into a plurality of overlapping DNA segments (240) and indexing information (250) added to the plurality of DNA segments. Finally, the plurality of DNA segments (240) is synthesized (790) and stored (795).Type: GrantFiled: October 29, 2021Date of Patent: February 6, 2024Assignee: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Nick Goldman, John Birney
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Patent number: 11834713Abstract: The present invention pertains to novel analgesics useful for treating mechanical pain. The invention suggests the use of inhibitors of ?-tubulin acetylation for inhibition of neurological sensations that are mediated by sensory neurons. The perception of mechanical pain is can be modulated by altering the ?-tubulin acetylation, in context of the invention in particular by modulation of the expression and/or activity of the enzyme ?-tubulin acetyltransferase (Atat). The invention provides the medical application of ?-tubulin acetyltransferase inhibitors as analgesics and a screening method for the identification of compounds useful in the treatment of pain.Type: GrantFiled: July 23, 2021Date of Patent: December 5, 2023Assignee: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Paul Heppenstall, Shane Morley
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Publication number: 20230375528Abstract: The present invention pertains to a method for identifying and/or characterizing a compound suitable for the prevention and/or treatment of a disease. The invention is based on the finding that the glycolytic enzyme Enolase 1 (ENO1) binds RNA, and its enzymatic activity is thereby regulated. The invention is further based on the finding that riboregulation of ENO1 affects cell differentiation, which plays a pivotal role in cancer. Accordingly, the invention provides a screening method for novel therapeutic compounds based on the binding of RNA to ENO1. Compounds screened according to the present invention can affect the binding of RNA to ENO1, which harbors the therapeutic potential for the treatment of diseases, in particular proliferative diseases, such as cancer. Methods of treatment using these compounds, as well as pharmaceutical compositions thereof, are also provided.Type: ApplicationFiled: October 6, 2021Publication date: November 23, 2023Applicant: European Molecular Biology LaboratoryInventors: Matthias W. HENTZE, Ina HUPPERTZ
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Publication number: 20230325308Abstract: A method for storage of an item of information (210) is disclosed. The method comprises encoding bytes (720) in the item of information (210), and representing using a schema the encoded bytes by a DNA nucleotide to produce a DNA sequence (230). The DNA sequence (230) is broken into a plurality of overlapping DNA segments (240) and indexing information (250) added to the plurality of DNA segments. Finally, the plurality of DNA segments (240) is synthesized (790) and stored (795).Type: ApplicationFiled: October 29, 2021Publication date: October 12, 2023Applicant: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Nick Goldman, John Birney
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Patent number: 11766427Abstract: The present invention relates to agents and compositions for the modification of the growth of bacterial cells. Thus, the compounds of the present invention are useful for the prevention and/or treatment of a disease in a subject. In particular, the present invention relates to the field of repurposing pharmaceutical compounds for treatment strategies of infectious diseases, gastrointestinal disorders, inflammatory diseases, proliferative diseases, metabolic disorders, cardiovascular diseases, and immunological diseases. Some of the compounds of the present invention demonstrate high specificity in inhibiting the growth of single bacterial species. Such compounds enable narrow-spectrum antibacterial therapies, constituting a major effort of current and future drug development strategies in order to reduce side effects of antibacterial treatment plans.Type: GrantFiled: February 13, 2019Date of Patent: September 26, 2023Assignee: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Mihaela Pruteanu, Lisa Maier, Michael Kuhn, Peer Bork, Athanasios Typas, Kiran Raosaheb Patil, Georg Zeller
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Publication number: 20230281112Abstract: A method for storage of an item of information (210) is disclosed. The method comprises encoding bytes (720) in the item of information (210), and representing using a schema the encoded bytes by a DNA nucleotide to produce a DNA sequence (230). The DNA sequence (230) is broken into a plurality of overlapping DNA segments (240) and indexing information (250) added to the plurality of DNA segments. Finally, the plurality of DNA segments (240) is synthesized (790) and stored (795).Type: ApplicationFiled: October 29, 2021Publication date: September 7, 2023Applicant: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Nick Goldman, John Birney
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Publication number: 20230272365Abstract: The invention relates to archaeal pyrrolysyl tRNA synthetases lacking a nuclear localization signal and/or comprising a nuclear export signal. The invention also relates to polynucleotides encoding said pyrrolysyl tRNA synthetases, eukaryotic cells comprising said polynucleotide and tRNA acylated by the pyrrolysyl tRNA synthetase or a polynucleotide encoding such tRNA, methods utilizing said cells for preparing polypeptides comprising unnatural amino acid residues, and kits useful in said methods.Type: ApplicationFiled: September 27, 2022Publication date: August 31, 2023Applicant: European Molecular Biology LaboratoryInventors: Edward Lemke, Ivana Nikic, Gemma Estrada Girona, Christine Köhler
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Publication number: 20230214319Abstract: A method for storage of an item of information (210) is disclosed. The method comprises encoding bytes (720) in the item of information (210), and representing using a schema the encoded bytes by a DNA nucleotide to produce a DNA sequence (230). The DNA sequence (230) is broken into a plurality of overlapping DNA segments (240) and indexing information (250) added to the plurality of DNA segments. Finally, the plurality of DNA segments (240) is synthesized (790) and stored (795).Type: ApplicationFiled: July 2, 2019Publication date: July 6, 2023Applicant: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Nick Goldman, John Birney
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Patent number: 11524294Abstract: The present invention relates to the field of microfluidics and in particular to devices and methods for sorting objects in microfluidic channels. These devices and methods allow for fast and robust sorting in two-way and multi-way setups. They also enable sorting over extended periods of time.Type: GrantFiled: September 20, 2017Date of Patent: December 13, 2022Assignee: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Christoph A. Merten, Daniel Frenzel
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Patent number: 11492608Abstract: The invention relates to archaeal pyrrolysyl tRNA synthetases lacking a nuclear localization signal and/or comprising a nuclear export signal. The invention also relates to polynucleotides encoding said pyrrolysyl tRNA synthetases, eukaryotic cells comprising said polynucleotide and tRNA acylated by the pyrrolysyl tRNA synthetase or a polynucleotide encoding such tRNA, methods utilizing said cells for preparing polypeptides comprising unnatural amino acid residues, and kits useful in said methods.Type: GrantFiled: October 13, 2017Date of Patent: November 8, 2022Assignee: European Molecular Biology LaboratoryInventors: Edward Lemke, Ivana Nikic, Gemma Estrada Girona, Christine Köhler
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Patent number: 11454626Abstract: The present invention relates to a method for single-cell imaging mass spectrometry (MS) by correlating an optical image of a cell sample with an MS image. The method of the invention is in particular useful in research to test concomitantly optical and molecular phenotypes at a single-cell resolution.Type: GrantFiled: April 13, 2018Date of Patent: September 27, 2022Assignee: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Theodore Alexandrov, Luca Rappez
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Patent number: 11441132Abstract: The present invention relates to improved transposase polypeptides having increased solubility. The enzyme of the invention was developed based on the Sleeping Beauty (SB) transposase. The invention provides further nucleic acids, vectors and recombinant cells encoding or containing the improved transposase, as well as a transposase system. Furthermore provided are medical and non-medical uses of the transposase of the invention for gene delivery. The invention is in particular useful as a tool for gene delivery in genetically modified cell based therapeutic approaches for treating various diseases.Type: GrantFiled: August 17, 2018Date of Patent: September 13, 2022Assignee: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Orsolya Barabas, Irma Querques, Cecilia Ines Zuliani
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Patent number: 11371917Abstract: The present disclosure teaches an apparatus and a method for providing one or more substance liquids to a microfluidic channel network. The microfluidic apparatus includes valves for switching the one or more substance liquids to a microfluidic channel network. The apparatus can be used to generate a sequence of the one or more substance liquids as individual droplets in an immiscible separation liquid wherein individual ones of the sequence of droplets are located between the separation liquid.Type: GrantFiled: January 9, 2019Date of Patent: June 28, 2022Assignee: European Molecular Biology LaboratoryInventors: Christoph Merten, Ramesh Utharala
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Publication number: 20220162267Abstract: The present invention relates to new adenoviral coat protein based delivery vehicles. They are based on a modified penton base protomers that assemble into VLPs. Exposed areas of the penton base proteins can be modified to allow the VLP to specifically bind to any target and/or to comprise any desired peptide epitope. Additional cargo, e.g. drugs, proteins, or nucleic acids, can reversibly or irreversibly attached to the VLP via engineered fibre protein fragments.Type: ApplicationFiled: February 1, 2022Publication date: May 26, 2022Applicants: THE EUROPEAN MOLECULAR BIOLOGY LABORATORY, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)Inventors: Imre BERGER, Frédéric GARZONI, Pascal FENDER
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Publication number: 20220145332Abstract: The Sleeping Beauty (SB) transposon is an efficient non-viral tool for inserting transgenes into cells. Its broad utilization in gene therapy has been hampered by uncontrolled transposase gene activity and the inability to use transposase protein directly. The present invention concerns the finding that SB transposase spontaneously penetrates mammalian cells and can be delivered with transposon DNA to gene-modify various cell lines, embryonic, hematopoietic and induced pluripotent stem cells. The invention provides methods and compounds to apply the cell penetrating function of transposase in methods of genetically engineering cells as well as using the transposase as a shuttle for delivering cargo into a target cell or even into a target cell organelle. Genomic integration frequency can be titrated using the technology of the invention, which adds an additional layer of safety, opening opportunities for advanced applications in genetic engineering and gene therapy.Type: ApplicationFiled: February 19, 2020Publication date: May 12, 2022Applicants: European Molecular Biology Laboratory, Julius-Maximilians-Universität WürzburgInventors: Michael HUDECEK, Andreas MADES, Orsolya BARABAS, Cecilia Ines ZULIANI, Irma QUERQUES
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Patent number: 11307020Abstract: A sample thickness measuring arrangement and method for measuring a thickness of a sample are proposed. The sample thickness measuring arrangement comprises a sample thickness measuring unit for measuring the thickness of a sample based on interferometry. Furthermore, it comprises a cryostat comprising a coolant reservoir and a sample observation unit for observing characteristics of a sample. The sample observation unit comprises a tube enclosing an observation volume, a thermal tube insulation layer and a window arrangement. The tube is open at a distal end and at a proximal end. The distal end of the tube is arranged. within a storage volume of the coolant reservoir. The tube has two lateral openings in its tube walls at opposing sides with regards to the observation volume. The tube walls are covered at their external side with the thermal tube insulation layer having two openings adjacent to the openings in the tube walls.Type: GrantFiled: September 13, 2019Date of Patent: April 19, 2022Assignee: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Florent Cipriani, Gergely Papp
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Patent number: 11274127Abstract: An engineered adenovirus penton base protomer, wherein the penton base protomer comprises a first RGD-loop, a second RGD-loop, a variable loop (V loop), an Adenovirus fiber protein binding cleft, and an N-terminal domain; wherein at least one of the first RGD-loop, the second RGD-loop, and the V loop comprises at least one inserted non-adenoviral antigenic polypeptide; and wherein the engineered adenovirus penton base protomer is capable of assembling into virus-like particles (VLPs).Type: GrantFiled: March 31, 2017Date of Patent: March 15, 2022Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), THE EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Imre Berger, Frédéric Garzoni, Pascal Fender
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Publication number: 20220043743Abstract: A method for storage of an item of information (210) is disclosed. The method comprises encoding bytes (720) in the item of information (210), and representing using a schema the encoded bytes by a DNA nucleotide to produce a DNA sequence (230). The DNA sequence (230) is broken into a plurality of overlapping DNA segments (240) and indexing information (250) added to the plurality of DNA segments. Finally, the plurality of DNA segments (240) is synthesized (790) and stored (795).Type: ApplicationFiled: October 29, 2021Publication date: February 10, 2022Applicant: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Nick Goldman, John Birney
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Publication number: 20220043744Abstract: A method for storage of an item of information (210) is disclosed. The method comprises encoding bytes (720) in the item of information (210), and representing using a schema the encoded bytes by a DNA nucleotide to produce a DNA sequence (230). The DNA sequence (230) is broken into a plurality of overlapping DNA segments (240) and indexing information (250) added to the plurality of DNA segments. Finally, the plurality of DNA segments (240) is synthesized (790) and stored (795).Type: ApplicationFiled: October 29, 2021Publication date: February 10, 2022Applicant: EUROPEAN MOLECULAR BIOLOGY LABORATORYInventors: Nick Goldman, John Birney