Patents Assigned to The General Hospital Corporation
  • Publication number: 20240060963
    Abstract: The present invention provides methods and compositions based on a non-naturally occurring nucleic acid construct encoding a fusion protein for quantitating levels of secretion in a single cell which may comprise a protein sequence which may comprise a cytoplasmic domain, a transmembrane domain and a vesicular domain, wherein the vesicular domain may comprise a protein tag sequence, wherein upon expression of the fusion protein by a cell, the fusion protein localizes to the membrane of a secretory vesicle such that the protein tag localizes to the lumen of the secretory vesicle, and wherein the protein tag binds to a cell-impermeable marker; whereby upon secretion of the contents of the secretory vesicle, the protein tag is exposed to the cell-impermeable marker, the fusion protein is recycled back into the cell, and the single cell becomes labeled with the marker relative to the amount of secretion.
    Type: Application
    Filed: August 9, 2023
    Publication date: February 22, 2024
    Applicants: THE GENERAL HOSPITAL CORPORATION, THE BROAD INSTITUTE, INC.
    Inventors: Sean Burns, Jason Wright, Thomas Sundberg
  • Patent number: 11903626
    Abstract: A fastener includes an elongate fastener body defining a longitudinal axis substantially central thereto. The fastener body has a continuous outer fastener shell laterally surrounding the longitudinal axis. The fastener shell longitudinally separates a fastener head end and a fastener tip end. A tool-engaging feature is provided on the fastener head end. A plurality of teeth are located on the fastener shell and extend substantially laterally outward from the longitudinally axis. The fastener shell is comprised of at least one undulate face and at least one substantially flat face. The plurality of teeth are located only on the undulate faces. Each tooth is longitudinally separated from adjacent teeth along an undulate face. A method of installing the fastener into a receiving structure is also described.
    Type: Grant
    Filed: May 4, 2021
    Date of Patent: February 20, 2024
    Assignees: THE GENERAL HOSPITAL CORPORATION, THE FEINSTEIN INSTITUTES FOR MEDICAL RESEARCH
    Inventors: Stanley E. Asnis, Peter D. Asnis
  • Publication number: 20240052428
    Abstract: The invention provides compositions and methods useful in characterizing and/or treating classical Hodgkin's Lymphoma and/or primary mediastinal B-cell lymphoma (PMBL). In embodiments, the characterization is carried out using a biological sample comprising circulating tumor DNA (ctDNA) from a subject.
    Type: Application
    Filed: September 15, 2023
    Publication date: February 15, 2024
    Applicants: The Broad Institute, Inc., Dana-Farber Cancer Institute, Inc., The General Hospital Corporation
    Inventors: Margaret SHIPP, Gad GETZ, Bjoern CHAPUY, Kirsty WIENAND, Donald STEWART, Andrew DUNFORD, Mark MURAKAMI, Lee LAWTON
  • Patent number: 11899022
    Abstract: The disclosure features methods of identifying specific drug candidates for disease treatment, the methods including: for pairs of associated expressed proteins in a protein-protein interaction network for a plurality of biological samples, comparing relative concentration values of the associated proteins in each of the biological samples to identify outliers among a distribution of the relative concentration values, identifying a set of proteins involved in dysregulated protein-protein interactions in the network based on the outliers, and predicting an efficacy of one or more drug candidates from among a set of drug candidates for treating a disease based on the identified set of proteins and information about one or more of proteins, protein complexes, biological pathways, and functional modules targeted by the set of drug candidates.
    Type: Grant
    Filed: August 23, 2018
    Date of Patent: February 13, 2024
    Assignee: The General Hospital Corporation
    Inventors: Wilhelm Haas, Cyril Benes
  • Patent number: 11898203
    Abstract: Provided herein are methods and compositions for performing highly sensitive in vitro assays to define substrate preferences and off-target sites of nucleic-acid binding, modifying, and cleaving agents.
    Type: Grant
    Filed: April 17, 2019
    Date of Patent: February 13, 2024
    Assignee: The General Hospital Corporation
    Inventors: J. Keith Joung, Vikram Pattanayak, Karl Petri
  • Patent number: 11898209
    Abstract: This disclosure relates to new assay methods for analysis of circulating tumor cells (CTCs) in blood samples for detection, e.g., early detection, and/or monitoring of disease, e.g., cancer. The methods provide ultra-high sensitivity and specificity, and include the use of microfluidic isolation of CTCs and digital detection of RNA derived from the CTCs.
    Type: Grant
    Filed: October 8, 2020
    Date of Patent: February 13, 2024
    Assignee: The General Hospital Corporation
    Inventors: Daniel A. Haber, Ravi Kapur, Mehmet Toner, Shyamala Maheswaran, Xin Hong, David Tomoaki Miyamoto, Tanya Todorova, Sarah Javaid
  • Publication number: 20240042034
    Abstract: Described herein are compositions comprising, and methods for using, biocompatible cold slurries and methods of administering the same to provide reversible inhibition of peripheral nerves in a subject in need thereof.
    Type: Application
    Filed: October 16, 2023
    Publication date: February 8, 2024
    Applicant: The General Hospital Corporation
    Inventors: Lilit Garibyan, Richard Rox Anderson, William A. Farinelli, Emilia Javorsky
  • Publication number: 20240043915
    Abstract: The present disclosure relates to compositions and methods for detecting nucleic acid sequences (e.g., coding and non-coding RNAs; nuclear/genomic DNA; mtDNA; pathogen nucleic acids, etc.) in a tissue sample, specifically providing improved matrices and matrix-employing methods for performance of nucleic acid capture and amplification in a tissue sample in situ and/or in a manner that retains spatial location information for captured nucleic acids (including nucleic acid-associated macromolecules).
    Type: Application
    Filed: February 11, 2022
    Publication date: February 8, 2024
    Applicants: THE GENERAL HOSPITAL CORPORATION, PRESIDENT AND FELLOWS OF HARVARD COLLEGE, THE BROAD INSTITUTE, INC.
    Inventors: Evan Macosko, Nicolas Lapique, Michael Kim
  • Patent number: 11890348
    Abstract: The invention described herein relates to methods and compositions for treating cancer in a patient, or a tumor cell, by administering an effective amount of an antibody-anti-fugetactic agent complex.
    Type: Grant
    Filed: September 16, 2016
    Date of Patent: February 6, 2024
    Assignee: The General Hospital Corporation
    Inventor: Mark C. Poznansky
  • Patent number: 11891631
    Abstract: Fusion proteins comprising a DNA binding domain, e.g., a TAL effector repeat array (TALE) or zinc finger array, and a catalytic domain comprising a sequence that catalyzes histone demethylation, and methods of use thereof.
    Type: Grant
    Filed: April 5, 2019
    Date of Patent: February 6, 2024
    Assignee: The General Hospital Corporation
    Inventors: Jae Keith Joung, Eric M. Mendenhall, Bradley E. Bernstein, Deepak Reyon
  • Patent number: 11890077
    Abstract: Exemplary method and apparatus for diagnosing or characterizing an inflammation within an anatomical structure can be provided. For example, using at least one source arrangement, it is possible to provide at least one first electro-magnetic radiation to the anatomical structure at at least one first wavelength in vivo. With at least one detector arrangement, it is possible to detect at least one second electro-magnetic radiation at at least one second wavelength provided from the anatomical structure. The second radiation can be associated with the first radiation, and the first wavelength can be shorter than the second wavelength. The second radiation can be provided from the anatomical structure due to at least one change in the anatomical structure caused by the inflammation without providing an artificial fluorescence substance.
    Type: Grant
    Filed: January 28, 2021
    Date of Patent: February 6, 2024
    Assignee: The General Hospital Corporation
    Inventors: Hao Wang, Joseph A. Gardecki, Guillermo J. Tearney
  • Patent number: 11891593
    Abstract: Presented is an airway organ bioreactor apparatus, and methods of use thereof, as well as bioartificial airway organs produced using the methods, and methods of treating subjects using the bioartificial airway organs. The bioreactor comprises: an organ chamber: an ingres line connecting the organ chamber and a reservoir system and comprising an arterial line, a venous line and a tracheal line; an egress line connecting the chamber and the reservoir system, pumps in ingress and egress lines; a controller to control fluid exchange; a chamber pressure sensor connected to the organ chamber.
    Type: Grant
    Filed: August 29, 2022
    Date of Patent: February 6, 2024
    Assignee: The General Hospital Corporation
    Inventor: Harald C. Ott
  • Patent number: 11890356
    Abstract: Provided herein are compounds useful for binding to one or more histone deacetylase enzymes (HDACs). The present application further provides radiolabeled compounds useful as a radiotracer for position emission tomography imaging of HDAC. Methods for prepared unlabeled and labeled compounds, diagnostic methods, and methods of treating diseases associated HDAC are also provided.
    Type: Grant
    Filed: November 16, 2021
    Date of Patent: February 6, 2024
    Assignee: The General Hospital Corporation
    Inventors: Jacob Hooker, Changning Wang, Martin Georg Strebl-Bantillo, Johanna Kaarina Rokka
  • Publication number: 20240032855
    Abstract: An apparatus for determining a shape of a luminal sample including: a catheter including a lens, the catheter disposed within a strain-sensing sheath such that the lens rotates and translates; a structural imaging system optically coupled to the catheter; a strain-sensing system optically coupled to the catheter; and a controller coupled to the strain-sensing system and the structural imaging system. The controller determines: a first position of the catheter relative to the luminal sample at a first location within the strain-sensing sheath; a second position of the catheter relative to the luminal sample at a second location within the strain-sensing sheath; a first strain of the strain-sensing sheath at the first location; a second strain of the strain-sensing sheath at the second location; a local curvature of the luminal sample relative to the catheter; a local curvature of the catheter; and a local curvature of the luminal sample.
    Type: Application
    Filed: October 10, 2023
    Publication date: February 1, 2024
    Applicant: The General Hospital Corporation
    Inventors: Guillermo J. Tearney, Joseph A. Gardecki, Kanwarpal Singh
  • Patent number: 11883680
    Abstract: A system and method for treating a tissue is provided. The treatment causes a tightening or lifting of tissue. The method includes delivering a photochemical agent through a depth of the tissue to distribute the photochemical agent adjacent proteins within the tissue and irradiating the tissue with an electromagnetic irradiation at a wavelength that activates the photochemical agent, causing a protein response that brings fibers of the tissue closer together in order to one of reduce tissue laxity and tighten the tissue. A kit is provided to facilitate the method.
    Type: Grant
    Filed: April 25, 2022
    Date of Patent: January 30, 2024
    Assignee: The General Hospital Corporation
    Inventors: Michael McCormack, Robert Redmond, William Gerald Austen, Jr.
  • Patent number: 11885733
    Abstract: Systems and methods for modeling and detecting white blood cell population dynamic for diagnosis and treatment, e.g., of acute coronary syndrome or leukocytosis.
    Type: Grant
    Filed: March 4, 2022
    Date of Patent: January 30, 2024
    Assignee: The General Hospital Corporation
    Inventors: John M. Higgins, Anwesha Chaudhury
  • Patent number: 11883182
    Abstract: A system and a method is provided for assessing motion of a biological tissue of a subject including one or more superficial biological layers and a targeted biological layer. An optical perturbation is introduced within the one or more superficial biological layers but not within the targeted biological layer. A set of optical signal data is acquired preceding, during, or following the optical perturbation and, using the set of optical signal data, a set of optical characteristics is determined that is representative of light transiting the biological layers. Using the set of optical characteristics and a model of the biological layers, a target optical signal consistent with a target biological layer is separated and a movement of the desired biological tissue is determined using the target optical signal.
    Type: Grant
    Filed: July 12, 2022
    Date of Patent: January 30, 2024
    Assignee: The General Hospital Corporation
    Inventors: Quan Zhang, Gary Strangman
  • Patent number: 11878019
    Abstract: The present disclosure provides methods of increasing skin pigmentation in a subject in need thereof using salt-inducible kinase (SIK) inhibitors, such as macrocyclic compounds of Formula (I), bicyclic urea compounds of Formula (II), (III), and (IV), and compounds of Formula (V), (VI), (VI-A), or (VII). Also provided are pharmaceutical compositions, methods, and uses that include or involve a compound described herein.
    Type: Grant
    Filed: November 22, 2021
    Date of Patent: January 23, 2024
    Assignees: The General Hospital Corporation, Dana-Farber Cancer Institute, Inc.
    Inventors: David E. Fisher, Nisma Mujahid, Ryo Murakami, Nathanael S. Gray, Yanke Liang, Hwan Geun Choi
  • Patent number: 11878063
    Abstract: Described herein are engineered ligand that binds a cell surface receptor (e.g., GPCR), with improved affinity, potency, and specificity. By conjugating a sub-optimal ligand for a cell surface receptor (e.g., GPCR) to a targeting molecule that binds an epitope (natural or exogenous epitope) in the extracellular portion of the cell surface receptor (e.g., GPCR), the affinity, potency, and/or specificity of the sub-optimal ligand is enhanced.
    Type: Grant
    Filed: March 5, 2020
    Date of Patent: January 23, 2024
    Assignees: Children's Medical Center Corporation, The General Hospital Corporation
    Inventors: Ross Cheloha, Hidde L. Ploegh, Thomas J. Gardella
  • Patent number: 11879953
    Abstract: A method for evaluating gastric and upper small intestine motility and emptying in a gastrointestinal tract of a subject using magnetic resonance imaging includes administering a contrast agent to the subject; acquiring, using an MRI system, a set of MR data from the subject using a three-dimensional volumetric pulse sequence. The volumetric pulse sequence has a temporal resolution and a spatial resolution configured to facilitate measurement of volumetric and motility parameters. The method further includes reconstructing a set of images using the set of MR data, segmenting each image in the set of images to isolate a region of interest in the gastrointestinal tract of the subject, determining at least one volumetric and motility parameter based on the set of segmented images, and generating a report indicating the at least one volumetric or motility parameter.
    Type: Grant
    Filed: May 11, 2020
    Date of Patent: January 23, 2024
    Assignee: The General Hospital Corporation
    Inventors: Braden Kuo, Christopher Nguyen, Roberta Sclocco, Vitaly Napadow