Abstract: This invention describes the process of separating fructose preferentially from a mixture of glucose and fructose by using ion exchange membranes. More particularly it relates to the process comprising the steps of (1) passing a liquid mixture of fructose and glucose through a first feed chamber of an electro-osmosis cell comprising at least two chambers defined between ion exchange membranes having alternating high and low permeability coefficients with respect to each other, (2) passing a direct electric current transversely through said membranes and chambers in a direction to cause the fructose to pass from said feed chamber through said high permeability coefficient membrane into a second chamber with said fructose being substantially retained in the second chamber, and (3) recovering a glucose enriched and a fructose enriched effluent from the separate chambers.