Abstract: The complex chemistry underlying the extensive transformations involved in terpene indole alkaloid synthesis makes identification of the biosynthetic genes challenging. The present invention relates to methods for producing a terpene indole alkaloid derivative, comprising the steps of: (1) providing a terpene indole alkaloid; and (2) providing a first enzyme termed “Precondylocarpine Acetate Synthase” (PAS) or a functional variant or homologue thereof; and/or a second enzyme termed “Dehydroprecondylocarpine Acetate Synthase” (DPAS) or a functional variant or homologue thereof, and optionally providing further identified enzymes involved in this pathway. The invention also encompasses related kits, enzymes, expression vectors, host cells and plants.
Type:
Grant
Filed:
March 15, 2019
Date of Patent:
August 30, 2022
Assignee:
John Innes Centre
Inventors:
Sarah O'Connor, Lorenzo Caputi, Richard Payne, Jakob Franke, Scott Farrow
Abstract: An isolated nucleic acid encoding a nucleotide-binding and leucine-rich repeat (NLR) polypeptide including a zinc-finger BED domain, wherein expression of the NLR polypeptide in a plant confers or enhances resistance of the plant to a fungus.
Type:
Application
Filed:
April 9, 2019
Publication date:
December 16, 2021
Applicants:
JOHN INNES CENTRE, COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION, THE UNIVERSITY OF SYDNEY
Abstract: The complex chemistry underlying the extensive transformations involved in terpene indole alkaloid synthesis makes identification of the biosynthetic genes challenging. The present invention relates to methods for producing a terpene indole alkaloid derivative, comprising the steps of: (1) providing a terpene indole alkaloid; and (2) providing a first enzyme termed “Precondylocarpine Acetate Synthase” (PAS) or a functional variant or homologue thereof; and/or a second enzyme termed “Dehydroprecondylocarpine Acetate Synthase” (DPAS) or a functional variant or homologue thereof, and optionally providing further identified enzymes involved in this pathway. The invention also encompasses related kits, enzymes, expression vectors, host cells and plants.
Type:
Application
Filed:
March 15, 2019
Publication date:
September 2, 2021
Applicant:
John Innes Centre
Inventors:
Sarah O?Connor, Lorenzo Caputi, Richard Payne, Jakob Franke, Scott Farrow
Abstract: Novel polyketides and novel methods of efficiently producing both new and known polyketides, using recombinant technology, are disclosed. In particular, a novel host-vector system is described which is used to produce polyketide synthases which in turn catalyze the production of a variety of polyketides.
Type:
Grant
Filed:
March 5, 1999
Date of Patent:
June 4, 2002
Assignees:
The Leland Stanford Junior University, The John Innes Centre
Inventors:
Chaitan Khosla, David A. Hopwood, Susanne Ebert-Khosla, Robert McDaniel, Camilla Kao
Abstract: Novel polyketides and novel methods of efficiently producing both new and known polyketides, using recombinant technology, are disclosed. In particular, a novel host-vector system is described which is used to produce polyketide synthases which in turn catalyze the production of a variety of polyketides.
Type:
Grant
Filed:
October 14, 1997
Date of Patent:
October 5, 1999
Assignees:
The Leland Stanford Junior University, The John Innes Centre
Inventors:
Chaitan Khosla, David A. Hopwood, Suzanne Ebert-Khosla, Robert McDaniel, Hong Fu, Camilla Kao