Abstract: We used hierarchical clustering to examine gene expression profiles generated by serial analysis of gene expression (SAGE) in a total of nine normal lung epithelial cells and non-small cell lung cancers (NSCLC). Separation of normal and tumor samples, as well as histopathological subtypes, was evident using the 3,921 most abundant transcript tags. This distinction remained when just 115 highly differentially expressed transcript tags were used. Furthermore, these 115 transcript tags clustered into groups that were suggestive of the unique biological and pathological features of the different tissues examined. Adenocarcinomas were characterized by high-level expression of small airway-associated or immunologically related proteins, while squamous cell carcinomas overexpressed genes involved in cellular detoxification or antioxidation.
Type:
Grant
Filed:
August 16, 2002
Date of Patent:
February 19, 2008
Assignees:
The United States of America as Represented by the Department of Health and Human Services, Genzyme Corporation, The Johns Hopkins University of Medicine
Inventors:
Mariana Nacht, Tatiana Dracheva, David Sidransky, Stephen Madden, Jin Jen
Abstract: This invention provides methods for treating a mycobacterial infection by administering to an animal a pharmaceutical composition containing a compound having the formula R—SOn-Z-CO—Y, where R is an alkyl group having 6-20 carbons; Z is a radical selected from —CH2—, —O—, and —NH—, two of these radicals coupled together, or —CH2═CH2—; Y is —NH2, O—CH2—C6H5, —CO—CO—O—CH3, or O—CH3; and n is 1 or 2. It has been discovered that these compounds inhibit growth of microbial cells which synthesize &agr;-substitued. &bgr;-hydroxy fatty acids, particularly corynemycolic acid, nocardic acid, and mycolic acid. These compounds may be used to inhibit growth of mycobacterial cells, such as Mycobacterium tuberculosis, drug-resistant M. tuberculosis, M. avium intracellulare, and M. leprae.
Type:
Application
Filed:
January 27, 2004
Publication date:
September 23, 2004
Applicant:
The Johns Hopkins University of Medicine
Inventors:
Craig A. Townsend, James D. Dick, Gary R. Pasternack, Francis P. Kuhajda, Nicole M. Parrish
Abstract: A novel protein, fibroblast growth factor homologous factor-1 (FHF-1), the polynucleotide sequence encoding FHF-1, and the deduced amino acid sequence are disclosed. Also disclosed are diagnostic and therapeutic methods of using the FHF-1 polypeptide and polynucleotide sequences and antibodies which specifically bind to FHF-1.
Type:
Application
Filed:
January 16, 2003
Publication date:
November 27, 2003
Applicant:
THE JOHNS HOPKINS UNIVERSITY OF MEDICINE, a Maryland corporation
Inventors:
Jeremy Nathans, Philip M. Smallwood, Jennifer P. Macke