Abstract: A method for the ionization of polymers which comprises forming a thick layer of the polymer on a probe tip and then ionizing the polymer in a vacuum with laser irradiation. In a preferred embodiment, a moiety that readily photoionizes in an IR field is incorporated into the thick layer of polymer and the irradiation of the thus modified polymer is done with a CO.sub.2 IR laser. A method is also disclosed for the fabrication of polymer coatings which comprises accelerating the charged polymer ions generated by the ionization of a thick layer of polymer in a vacuum toward the substrate which has an appropriate charge gradient.

Abstract: A local probe for use in nuclear magnetic resonance imaging includes a pair of loop-gap resonators which are connected together and positioned adjacent a region of interest. FID signals produced in the region of interest induce signals in coupling loops mounted near each loop-gap resonator, and these connect to a transmission line leading to the receiver. Three embodiments are disclosed, one best suited for imaging appendages, a second best suited for imaging the head and neck, and a third best suited for the spine.

Abstract: Human myeloma fusing lines and human/human hybridomas produced therefrom are disclosed. In one embodiment, the myeloma cell line is HAT sensitive, does not secrete detectable levels of Epstein-Barr virus EBNA-I protein, and does not secrete or elaborate detectable levels of myeloma immunoglobulin. The myeloma cell line and resulting hybridoma are stable over time, and thus permit production of commercial quantities of human monoclonal antibody.

Abstract: An in vivo assay for quantifying the mineral content of bone comprises placing living bone into or adjacent to the coil of a nuclear magnetic resonance (NMR) spectrometer, recording a .sup.31 P NMR spectrum while the bone is stationary, and comparing the spectrum obtained with the NMR spectrum of a reference standard containing a known concentration of a reference compound. The assay can be used to diagnose and determine the efficacy of treatment of osteodystrophy if used in conjunction with radiographic densitometry to estimate the calcium content of the bone.

Abstract: The thickness of a web is measured by passing the web through the gaps of a loop-gap resonator. Changes in web thickness alter the resonant frequency of the resonator which is detected by a control circuit. Three embodiments of the invention are described, and in one embodiment, the moisture content of the web is also measured.

Abstract: Blood flow in human limbs is measured non-invasively by a nuclear magnetic resonance blood flowmeter which includes a pair of polarizing magnets whose fields are stabilized by electromagnets in each pole piece that are energized in accordance with the magnetic flux of the magnets as sensed by a Hall effect sensor. Blood molecules are either self-tagged by the polarizing field or are separately tagged by a tag coil whose field is orthogonal to the polarizing field. Once tagged, the variation in the magnetic moment of the molecule due to the tagging is detected by the variation in voltage in the receiver coil which is located orthogonally to the transmitter coil so as to reduce crosscoupling therebetween.

Abstract: An ESR spectrometer is employed to measure the oxygen concentration in a sample material. Electron paramagnetic resonance is produced in a free radical mixed with a standard material of known oxygen concentration. The phase of the signal produced by this resonance is compared with the phase of the signal produced by the electron paramagnetic resonance of the free radical mixed with the sample material. The oxygen concentration in the sample material has a single valued functional relationship with respect to this phase difference and is calculated in a microprocessor-based control system. At low oxygen concentrations, the relationship is a linear one.

Abstract: A method of identifying the matching nerve fascicles in proximal and distal nerve stumps for reanastomosis comprises histochemically making a first visible image of the pattern of carbonic anhydrase emitted by the distal end of the severed nerve or its trimming and a second image of the pattern of carbonic anhydrase emitted by the proximal end of the severed nerve or its trimming, then comparing the two images and thus identifying the fascicles to be joined. A kit for preparing the images is also disclosed.

Abstract: An assay method for the quantitative determination of the mineral content of bone comprises using proton decoupling and magic angle sample spinning techniques to record well resolved .sup.31 P nuclear magnetic resonance spectra of unfractionated bone biopsy samples and characterizing the differences in mineral identity and content from those of normal bone. A rotor for performing the assay includes a compartment for the sample to be assayed, and a separate compartment for a reference material.

Abstract: The ends of a loop-gap resonator are extended along a closed path to define an enclosed chamber with a gap extending along its length. A lumped circuit resonator is formed and the magnetic field is confined to the chamber. Openings may be formed in the chamber wall to direct magnetic flux through sample materials outside the chamber, and the cross sectional area of the chamber may be altered to control the energy density distribution of the magnetic field.

Abstract: A lumped circuit loop-gap resonator is formed by a plurality of openings formed through a block of material. The openings form inductive loop elements and these are interconnected in a variety of possible networks by capacitive gap elements. String, star, ring and lattice networks are described.

Abstract: A structure for supporting a loop-gap resonator includes a cylindrical housing which defines a space into which microwave energy is injected. The resonator is mounted to a support element which is inserted into the space through an opening at one end of the housing. The support element and attached resonator form an interchangeable module.

Abstract: A microwave resonator is formed by a cylindrical conductive loop and one or more gaps which extend along its length. The loop forms an inductive element and the gaps form capacitive elements which determine the resonant frequency. The loop is surrounded by a conductive shield which improves the quality factor (Q) of the resonator and high frequency radio signals are coupled to the resonator by an inductive loop positioned at one of its ends.

Abstract: A microwave resonator is formed by a cylindrical loop and one or more gaps which extend along its length. The loop is formed from a machineable insulating material and a layer of electrically conductive material is deposited over its surfaces.

Abstract: A method of detecting cancer and other abnormalities involves projecting the image of the cervix from the photograph onto a screen, viewing the screen from a short distance and noting the abnormalities.

Abstract: The loss of biological activity of interferons is prevented or reduced by the addition of soluble lanthanide or calcium salts.

Abstract: A competitive enzyme-linked immunoassay for a soluble analyte in which the analyte in the unknown sample and the same analyte attached to a solid phase (immunosorbent) compete for a selected quantity of a soluble first antibody to the substance; a second or bridging antibody is then linked to any first antibody linked to the immunosorbent as a result of the competitive reaction, following which a soluble enzyme/antibody immune complex is linked to bridging antibody on the immunosorbent. The enzymatic activity associated with the thusly-formed enzyme-immunosorbent complex or with the non-immunosorbent fraction of enzyme is measured to determine the presence and/or concentration of analyte in the unknown sample.

Abstract: An assay for proteins or polypeptides in solution using a reagent of Coomassie Brilliant Blue G250 dye in perchloric or hydrochloric acid. Upon mixture of the acidic reagent with a protein sample, the dye couples with the protein and undergoes a color change, following which the absorbance of the mixture can be measured to provide a quantitative analysis for the protein in the sample; the same procedure may be used for sample solutions containing polypeptides. Proteins and polypeptides with molecular weights above about 2000-3000 may be assayed.