Abstract: The present invention provides a promoter for inducing expression specifically in at least one site of roots and stem apices. By using this promoter, it is possible to synthesize a resistance substance against nematodes or soil pathogens in roots; synthesize a substance promoting the development of roots in the roots; produce a gene recombinant plant in which expression of a selective marker in the redifferentiated plant body is limited to roots when the plant is young, so as to suppress the expression when the plant is matured; or the like. Further, the capability of expression in stem apices can be used to develop a technique for suppress gene expression in vigorously proliferating cells.
Type:
Application
Filed:
January 25, 2002
Publication date:
October 12, 2006
Applicant:
National Institute of Agrobiological Sciences
Abstract: The present invention provides a gene encoding ALS protein which shows a extremely high level of resistance to pyrimidinyl carboxy herbicides. The gene of the present invention encodes an acetolactate synthase protein, for instance, according to SEQ ID NO:1, where the acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity.
Type:
Grant
Filed:
November 16, 2001
Date of Patent:
October 10, 2006
Assignees:
Kumiai Chemical Industry Co., Ltd., National Institute of Agrobiological Sciences
Abstract: It is an object to provide polypeptide compositions originating from silk protein which have not only excellent cell growth-promoting activity associated with undegraded silk fibroin and sericin, the fibroin H-chain and L-chain, or the a component of sericin, but also excellent touch, extensibility, and the like inherent in fibroin and sericin having a molecular weight not higher than 200,000.
Type:
Grant
Filed:
March 11, 2004
Date of Patent:
October 3, 2006
Assignee:
National Institute of Agrobiological Sciences
Abstract: A novel promoter is provided which has an expression activity specific to any pistil tissue. The promoter has any one of the DNA sequence from position 1 to 2595 of SEQ ID NO. 1, the DNA sequence from position 1 to 2322 of SEQ ID NO. 2, and the DNA sequence from position 1 to 2012 of SEQ ID NO. 3, and a part thereof. A method for producing a plant having a modified trait by introducing a heterogenous gene operatively linked to the promoter into a plant, and a plant having a modified trait produced by the method are further provided.
Type:
Grant
Filed:
May 21, 1999
Date of Patent:
August 29, 2006
Assignees:
National Institute of Agrobiological Sciences, Bio-Oriented Technology Research Advancement Institution
Abstract: The inventors investigated the impact of an 11-bp deletion in the swine Mx1 gene on the ability to suppress propagation of influenza viruses belonging to the myxovirus family, and revealed that the deletion led to a complete loss of the ability to suppress viral propagation. Through the detection of the 11 -bp deletion, pigs can be examined for their resistance to RNA viruses such as influenza viruses and the virus that causes PRRS.
Type:
Application
Filed:
October 28, 2003
Publication date:
July 27, 2006
Applicant:
National Institute of Agrobiological Sciences
Abstract: This invention provides a method for effectively producing dehydrated larvae for educational materials without disrupting the environment. The cryptobiotic larvae for educational materials can be obtained by dehydrating larvae while gradually reducing humidity in 3 separate stages.
Type:
Application
Filed:
June 13, 2005
Publication date:
July 20, 2006
Applicant:
National Institute of Agrobiological Sciences
Abstract: A novel cell culture medium suitable for primary culture of insect cells, an insect-derived water-soluble chitin, and a process of preparing an insect culture cell line in a short period of time by using the insect primary culture medium and the insect-derived water-soluble chitin. The insect cell primary culture medium comprises lactalbumin hydrolysate, yeastolate, and tryptose phosphate broth as protein extracts, and polyvinylpyrrolidone as a viscosity-supplementing agent. The insect-derived water-soluble chitin is subjected to deacetylation as the sole chemical modification.
Type:
Grant
Filed:
November 19, 2003
Date of Patent:
July 11, 2006
Assignee:
National Institute of Agrobiological Sciences
Inventors:
Shigeo Imanishi, Atsunobu Haga, Jun Mitsuhashi
Abstract: A polynucleotide encoding a plant gene capable of controlling disease resistance reactions in plants is provided which includes a polynucleotide having a nucleotide sequence encoding amino acid sequence from methionine at position 1 to Serine at position 361 of SEQ ID NO: 2 in the sequence listing, or having the amino acid sequence having one or several amino acid deletions, substitutions and/or additions, and being capable of controlling disease resistance reactions.
Type:
Grant
Filed:
November 26, 2002
Date of Patent:
June 27, 2006
Assignees:
National Institute of Agrobiological Sciences, Bio Oriented Technology Research Advancement Institution
Abstract: The present inventors studied intensively, concentrating on the hypersensitive reaction from among the various reactions involved in enhancing plant resistance. As a result, the inventors found AAM97746.1 to be a protein that induces hypersensitive reaction-like lesion mimic. Disease or pest resistance in plants can be enhanced by using this protein and the like.
Type:
Application
Filed:
November 28, 2005
Publication date:
June 15, 2006
Applicant:
National Institute Of Agrobiological Sciences
Abstract: The invention provides an in vitro culture medium for in vitro-produced porcine embryo for the in vitro culture thereof, which can improve the quality of the resulting blastocyst and can raise the ratio of the development into fetus and infant after transfer, along with a method for in vitro culturing in vitro-produced porcine embryo using the culture medium, which can improve the quality of the resulting blastocyst and can develop the blastocyst into fetus and infant after transfer. A culture medium for the in vitro culture of in vitro-produced porcine embryo, which contains lactic acid and pyruvic acid; a culture medium for the in vitro culture, which is conditioned with oviductal epithelial cell; and a method for in vitro culturing in vitro-produced porcine embryo, comprising culturing the in vitro-produced porcine embryo using the culture medium for the 0 to 2-day term after fertilization and subsequently culturing the embryo in a glucose-containing culture medium.
Type:
Grant
Filed:
March 2, 2004
Date of Patent:
June 13, 2006
Assignee:
National Institute of Agrobiological Sciences
Abstract: Genomic DNA and cDNA encoding GA 3?-hydroxylase were isolated from rice. When the expression of these genes was suppressed in rice plants, the plants became dwarfed compared with the wild type plants.
Type:
Grant
Filed:
December 20, 2000
Date of Patent:
May 23, 2006
Assignees:
National Institute of Agrobiological Sciences, Riken
Abstract: It is intended to provide thermotolerant and antimicrobial proteins having a broad antibacterial spectrum and being useful in the agricultural, medical and industrial field; and plants resistant to pathogenic microorganisms. Namely, DNAs comprising the base sequences represented by SEQ ID NOS: 1 to 5 respectively; antimicrobial proteins encoded by these DNAs; and fungicides, antibacterial agents and antibacterial/antifungal agents for industrial use comprising the antimicrobial proteins as the active ingredient.
Type:
Grant
Filed:
October 15, 2002
Date of Patent:
May 9, 2006
Assignee:
National Institute of Agrobiological Sciences
Inventors:
Tetsuya Tomie, Michito Tagawa, Minoru Yamakawa, Jun Ishibashi
Abstract: The present inventors successfully isolated a photoperiod sensitivity gene Hd6 from rice by a linkage analysis. It was revealed that the photoperiod sensitivity of plants can be modified by introducing the gene or controlling the expression of the gene. Further, it was demonstrated that the photoperiod sensitivity of plants can be assessed by detecting the presence or absence of the functional gene.
Type:
Grant
Filed:
November 1, 2000
Date of Patent:
May 2, 2006
Assignees:
National Institute of Agrobiological Sciences, Society for Techno-Innovation of Agriculture, Forestry and Fisheries, National Agriculture and Bio-Oriented Research Organization
Abstract: An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof. cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins (PvLEA1, PvLEA2, and PvLEA3). When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had ?-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moerover, the recombinant proteins synthesized from PvLEA1, 2 and 3 genes were heat soluble even when boiling, so that PvLEA1, 2 and 3 proteins have highly hydrophilic property as well as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes.
Type:
Application
Filed:
September 8, 2005
Publication date:
March 16, 2006
Applicant:
National Institute of Agrobiological Sciences
Abstract: Wheat starch of the present invention is obtained from endosperm of a seed of wheat which is modified to lack starch granule protein-1 (SGP-1). The wheat starch has an apparent amylose content of about 35% or more. Wheat flour of the present invention is obtained from endosperm of a seed of wheat which is modified to lack SGP-1. Wheat of the present invention is modified to lack SGP-1. The wheat flour and the wheat comprise wheat starch which has an apparent amylose content of about 35% or more.
Type:
Grant
Filed:
April 24, 2001
Date of Patent:
February 21, 2006
Assignee:
National Institute of Agrobiological Sciences
Abstract: There is provided a polynucleotide encoding a plant gene capable of controlling a signal transduction system for brassinosteroid hormone, the polynucleotide encoding an amino acid sequence from Met at position 1 to Arg at position 1057 of SEQ ID NO: 2 in the SEQUENCE LISTING, including any polynucleotide encoding an amino acid sequence in which one or more amino acids are deleted, substituted or added to the amino acid sequence.
Type:
Grant
Filed:
November 22, 2000
Date of Patent:
January 10, 2006
Assignees:
National Institute of Agrobiological Science, Bio-oriented Technology Research Advancement Institution
Abstract: A tissue of a multicellular organism is gradually dried during cultivation. After the tissue has been completely dehydrated, water is added to the tissue for its recovery. The tissue of the multicellular organism is submerged in an insect body fluid medium treated with heat, and dried for 48 hours or more.
Type:
Grant
Filed:
September 11, 2003
Date of Patent:
November 8, 2005
Assignees:
National Institute of Agrobiological Sciences
Abstract: A method for identifying resistance of a rice plant to rice blast is provided. More particularly, a method for identifying resistance of a rice plant to rice blast by testing a genotype of the rice genome using a DNA marker (G271), which is closely linked to a gene controlling the field resistance to rice blast, is disclosed. The disclosed invention allows for evaluation of the field resistance of a rice plant to rice blast using a DNA marker, thereby allowing for a resistant variety to be conveniently and accurately selected. The disclosed invention contributes to reducing the time and labor that have conventionally been required for cross breeding, and is useful in developing novel rice varieties having a high degree of field resistance to rice blast.
Type:
Grant
Filed:
April 28, 2000
Date of Patent:
November 8, 2005
Assignee:
National Institute of Agrobiological Sciences
Abstract: The present invention enables characteristic gene expression control and relates to a promoter, which comprises any one of the following DNAs (a) to (c): (a) a DNA, which comprises the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which comprises a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID NO: 1 by deletion, substitution, insertion, or addition of 1 or a plurality of nucleotides, and which can control transcription of a gene located downstream thereof; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment comprising a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1, and which can control the transcription of a gene located downstream thereof.
Type:
Application
Filed:
March 10, 2005
Publication date:
October 27, 2005
Applicants:
Kumiai Chemical Industry Co., Ltd., National Institute of Agrobiological Sciences
Abstract: A novel cell culture medium suitable for primary culture of insect cells, an insect-derived water-soluble chitin, and a process of preparing an insect culture cell line in a short period of time by using the insect primary culture medium and the insect-derived water-soluble chitin. The insect cell primary culture medium comprises lactalbumin hydrolysate, yeastolate, and tryptose phosphate broth as protein extracts, and polyvinylpyrrolidone as a viscosity-supplementing agent. The insect-derived water-soluble chitin is subjected to deacetylation as the sole chemical modification.
Type:
Grant
Filed:
March 6, 2002
Date of Patent:
September 13, 2005
Assignee:
National Institute of Agrobiological Sciences
Inventors:
Shigeo Imanishi, Atsunobu Haga, Jun Mitsuhashi