Abstract: Methods for stably transfecting mammalian natural killer cells comprising: transfecting a packaging cell line with a retroviral expression vector; culturing the transfected packaging cell line in a cell culture medium; and culturing the mammalian natural killer cells with the cell culture medium. Natural killer cells transfected according to the disclosed methods are also provided.
Type:
Application
Filed:
March 21, 2005
Publication date:
December 4, 2008
Applicant:
The Ohio State Univeristy Research Foundation
Inventors:
Michael A. Caligiuri, Rossana Trotta, Jianhua Yu, Brian Becknell